Immunomodulatory LncRNA on antisense strand of ICAM-1 augments SARS-CoV-2 infection-associated airway mucoinflammatory phenotype

Devadoss, Dinesh; Acharya, Arpan; Manevski, Marko; Houserova, Dominika; Cioffi, Michael; Pandey, Kabita; Nair, M. G.; Chapagain, Prem; Mirsaeidi, Mehdi; Borchert, Glen M.; Byrareddy, Siddappa N.; Chand, Hitendra S.

doi:10.1016/j.isci.2022.104685

Cited by 12 publications

(7 citation statements)

References 82 publications

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“…Accumulating evidence has shown that lncRNAs can regulate the immune system, including inflammatory responses [ 81 ], and play an important role in mucosal inflammation [ 82 ]. In our work, around 10% of the DEGs were lncRNAs, and our interactome analysis identified 27 of them ( Figure 5 ).…”

Section: Discussionmentioning

confidence: 99%

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Buffy Coat Transcriptomic Analysis Reveals Alterations in Host Cell Protein Synthesis and Cell Cycle in Severe COVID-19 Patients

Cavalcante

Fonseca²,

Leon³

et al. 2022

IJMS

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Transcriptome studies have reported the dysregulation of cell cycle-related genes and the global inhibition of host mRNA translation in COVID-19 cases. However, the key genes and cellular mechanisms that are most affected by the severe outcome of this disease remain unclear. For this work, the RNA-seq approach was used to study the differential expression in buffy coat cells of two groups of people infected with SARS-CoV-2: (a) Mild, with mild symptoms; and (b) SARS (Severe Acute Respiratory Syndrome), who were admitted to the intensive care unit with the severe COVID-19 outcome. Transcriptomic analysis revealed 1009 up-regulated and 501 down-regulated genes in the SARS group, with 10% of both being composed of long non-coding RNA. Ribosome and cell cycle pathways were enriched among down-regulated genes. The most connected proteins among the differentially expressed genes involved transport dysregulation, proteasome degradation, interferon response, cytokinesis failure, and host translation inhibition. Furthermore, interactome analysis showed Fibrillarin to be one of the key genes affected by SARS-CoV-2. This protein interacts directly with the N protein and long non-coding RNAs affecting transcription, translation, and ribosomal processes. This work reveals a group of dysregulated processes, including translation and cell cycle, as key pathways altered in severe COVID-19 outcomes.

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Section: Discussionmentioning

confidence: 99%

“…In addition, when analyzed individually, the expression level of lncRNA NEAT1 was significantly correlated with the SARS-CoV-2 viral RNA level. These data suggest that NEAT1 lncRNA may play a role in the innate airway responses and may be involved in SARS-CoV-2-associated airway inflammation [ 82 ].…”

Section: Discussionmentioning

confidence: 99%

Buffy Coat Transcriptomic Analysis Reveals Alterations in Host Cell Protein Synthesis and Cell Cycle in Severe COVID-19 Patients

Cavalcante

Fonseca²,

Leon³

et al. 2022

IJMS

View full text Add to dashboard Cite

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“…We next analyzed the effects of WS-23 aerosols on AEC mRNA expression of inflammatory factors, IL-6, and ICAM-1, which are important modulators of AEC innate responses [5] , [29] . At 48 h of treatment, IL-6 mRNA levels were significantly reduced by WS-23 or nicotine aerosol exposure, as the WS-23, WS-23 + nicotine, and nicotine alone aerosol treated groups presented with 2.0-fold or higher reduction in IL-6 mRNA levels, compared to PG/VG-treated controls ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The respiratory airway epithelial cells (AECs) are pivotal to innate immune defense against inhaled toxicants/allergens, and the AEC responses to aerosolized e-cig components are crucial for orchestrating the lung immune responses [8] , [30] . Any dysregulation in AEC-mediated responses can significantly impact the susceptibility to infection [6] ; a recent study has shown that e-cig use induces a reduction in AEC ciliary beating frequency, as well as changes in cytokine and chemokine production [18] . Nevertheless, the impact of synthetic cooling agents such as WS-23 individually has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

E-cigarette synthetic cooling agent WS-23 and nicotine aerosols differentially modulate airway epithelial cell responses

Manevski

Yogeswaran²,

Rahman³

et al. 2022

Toxicology Reports

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“…In line with the above, our findings reported a drop in mucin-16 [Q8WXI7] levels in the COVID-19 group compared to uninfected controls. A study conducting a transcriptome-wide correlation aiming to unveil the impact of gene expression on COVID-19 course revealed that up-regulated expression of MUC1 was associated with critical disease [ 29 ], while MUC5AC and MUC5B mucins have also been found to be elevated in severe COVID-19 [ 30 , 31 ]. Our results attest to a forefront role of this molecule on COVID-19 pathobiology.…”

Section: Discussionmentioning

confidence: 99%

Serum Proteome Signatures of Anti-SARS-CoV-2 Vaccinated Healthcare Workers in Greece Associated with Their Prior Infection Status

Stamoula

Sarantidi

Dimakopoulos

et al. 2022

IJMS

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Over the course of the pandemic, proteomics, being in the frontline of anti-COVID-19 research, has massively contributed to the investigation of molecular pathogenic properties of the virus. However, data on the proteome on anti-SARS-CoV-2 vaccinated individuals remain scarce. This study aimed to identify the serum proteome characteristics of anti-SARS-CoV-2 vaccinated individuals who had previously contracted the virus and comparatively assess them against those of virus-naïve vaccine recipients. Blood samples of n = 252 individuals, out of whom n = 35 had been previously infected, were collected in the “G. Gennimatas” General Hospital of Thessaloniki, from 4 January 2021 to 31 August 2021. All participants received the BNT162b2 mRNA COVID-19 vaccine (Pfizer/BioNTech). A label-free quantitative proteomics LC-MS/MS approach was undertaken, and the identified proteins were analyzed using the GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes) databases as well as processed by bioinformatics tools. Titers of total RBD-specific IgGs against SARS-CoV-2 were also determined using the SARS-CoV-2 IgG II Quant assay. A total of 47 proteins were significantly differentially expressed, the majority of which were down-regulated in sera of previously infected patients compared to virus-naïve controls. Several pathways were affected supporting the crucial role of the humoral immune response in the protection against SARS-CoV-2 infection provided by COVID-19 vaccination. Overall, our comprehensive proteome profiling analysis contributes novel knowledge of the mechanisms of immune response induced by anti-SARS-CoV-2 vaccination and identified protein signatures reflecting the immune status of vaccine recipients.

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Immunomodulatory LncRNA on antisense strand of ICAM-1 augments SARS-CoV-2 infection-associated airway mucoinflammatory phenotype

Cited by 12 publications

References 82 publications

Buffy Coat Transcriptomic Analysis Reveals Alterations in Host Cell Protein Synthesis and Cell Cycle in Severe COVID-19 Patients

Buffy Coat Transcriptomic Analysis Reveals Alterations in Host Cell Protein Synthesis and Cell Cycle in Severe COVID-19 Patients

E-cigarette synthetic cooling agent WS-23 and nicotine aerosols differentially modulate airway epithelial cell responses

Serum Proteome Signatures of Anti-SARS-CoV-2 Vaccinated Healthcare Workers in Greece Associated with Their Prior Infection Status

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