Immunopathological Diagnosis of Pemphigus foliaceus  /  Reply

Jarzabek-Chorzelska, M; Jabłońska, S; Kolacinska-Strasz, Z; Sulej, I

doi:10.1159/000066438

Cited by 11 publications

(7 citation statements)

References 4 publications

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“…The period of recruitment started in 1998 in northern regions and in 2002 in southern areas. All patients with PF were diagnosed on clinical, histological and immunological criteria 10 and were matched by age (± 5 years), sex and geographical origin to 270 healthy controls (130 from the south and 140 from the north). All patients and healthy controls gave written consent to participate in the study.…”

Section: Methodsmentioning

confidence: 98%

Tunisian endemic pemphigus foliaceus is associated with the HLA-DR3 gene: anti-desmoglein 1 antibody-positive healthy subjects bear protective alleles

Abida¹,

Zitouni²,

Kallel-Sellami³

et al. 2009

British Journal of Dermatology

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Section: Methodsmentioning

confidence: 98%

Tunisian endemic pemphigus foliaceus is associated with the HLA-DR3 gene: anti-desmoglein 1 antibody-positive healthy subjects bear protective alleles

Abida¹,

Zitouni²,

Kallel-Sellami³

et al. 2009

British Journal of Dermatology

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“…The diagnosis of PF was established according to the standard clinical, histological and immunological criteria of the disease [24]. All specimens were taken from PF patients with active disease and before treatment (T0).…”

Section: Subjectsmentioning

confidence: 99%

Potential Role of TLR Ligand in Aethiopathogenesis of Tunisian Endemic Pemphigus Foliaceus

D¹

2013

Biochem Physiol

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Pemphigus foliaceus (PF) is an autoimmune skin disease in which environmental factors are thought to participate. Recent studies suggest that microbial components use signaling molecules of the human Toll-like receptor (TLR) family to transduce signals in keratinocytes. The aim of our research was to investigate the expression of TLRs 2, 3 and 4 by keratinocytes of PF patients compared to normal keratinocytes, in order to characterise the nature of the microbial factor involved in the etiopathology of PF. Biopsies obtained from 43 PF patients and 20 healthy controls were assessed by immunohistochemical analysis using specific polyclonal antibodies. The TLR2, TLR3 and TLR4 expression was significantly upregulated in PF epidermis. The significant increase of those TLRs simultaneously may merely reflect the complicated environmental conditions of rural women in the southern rural regions of Tunisia. Interestingly, we have found that the TLR4 diffuse expression was associated with the production of anti-desmoglein 1 Abs (p=0.037). This could be in line with a potential role of TLR ligand in aethiopathogenesis of Tunisian endemic PF.TLR over-expression in pemphigus skin indicates that TLRs are involved in the pathogenesis of pemphigus through stimulation by infectious or endogenous ligands.

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“…(iii) Finally, once an initial autoantibody response is triggered against one of these cadherins (primary response), this . Domains EC1 to EC5 of human Dsg1 or Dsg3 were domain-swapped with the corresponding domains of human Dsg2 to generate five recombinant molecules [18]. (B) Representative results of epitope mapping of patients (P1 and P2) and Healthy donors (T1, T2, T3 and T4) sera.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we used sera from 2 Tunisian patients with endemic PF (PF) and 4 healthy blood donors found in previous studies reported by our group to have anti-Dsg1 and anti-Dsg3 Abs [17] (Table 1). PF patients were diagnosed on clinical, histological (acantholysis in the upper epidermis either in the granular layer or immediately below with subcorneal bullous formation) and immunological criteria (cell surface staining) of PF [18].…”

Section: Population's Demographics Characteristicsmentioning

confidence: 99%

Antibodies against Desmoglein 1, Desmoglein 3 and BP180 in Healthy Tunisians Blood Donors without Immunopathological Phenotype

Toumi¹

2016

CDT

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Pemphigus Vulgaris (PV), Pemphigus Foliaceus (PF) and Bullous Pemphigoid (BP) are a group of rare organ-specific diseases that affect skin and mucous membranes. Each subtype of pemphigus has its own anti-Desmogleins (Dsgs) autoantibodies profile: Dsg1 is the auto-antigen in PF whereas Dsg3 is the in PV. We identified 2 PF patients with positive levels of anti-Dsg3 Abs and also 4 healthy blood donors with high levels of anti-Dsg1 Abs and anti-Dsg3 Abs. The purpose of this study was to examine epitopes recognized by anti-Dsg1/anti-Dsg3 Abs and to identify potential autoreactive HLAII-restricted helper T cell epitopes shared among Dsg1+/Dsg3+ subjects that could explain the simultaneous occurrence of these auto-Abs. On IIF, only one PF patients had antibodies to the keratinocyte surface. Interestingly, the ELISA for BP180 was also positive in 2 healthy blood donors. IP-IB analysis showed immunoreactivity to more than one EC domain of Dsg1 and towards the C-terminal EC5 domain of Dsg3. MHC II gene analysis demonstrated that all healthy blood donors had at least one protective allele associated to PF. We confirm in this study that the onset and course of this disease depend on a variable interaction between predisposing and inducing genetic, environmental, hormonal, and immunological factors.

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Immunopathological Diagnosis of Pemphigus foliaceus / Reply

Cited by 11 publications

References 4 publications

Tunisian endemic pemphigus foliaceus is associated with the HLA-DR3 gene: anti-desmoglein 1 antibody-positive healthy subjects bear protective alleles

Tunisian endemic pemphigus foliaceus is associated with the HLA-DR3 gene: anti-desmoglein 1 antibody-positive healthy subjects bear protective alleles

Potential Role of TLR Ligand in Aethiopathogenesis of Tunisian Endemic Pemphigus Foliaceus

Antibodies against Desmoglein 1, Desmoglein 3 and BP180 in Healthy Tunisians Blood Donors without Immunopathological Phenotype

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