Immunopathology of experimental Brucella abortus strain 19 infection of the genitalia of bulls

Campero, C.M.; Ladds, P. W.; Hoffmann, D.; Duffield, B.J.; Watson, D. J.; Fordyce, Geoffry

doi:10.1016/0165-2427(90)90039-u

Cited by 23 publications

(16 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The distribution of IgCC in control and infected sheep glands observed in this work is consistent with the findings demonstrated in bulls, where IgCC are also not found or are present in a very limited number in the seminal vesicles of normal animals (Campero et al 1989(Campero et al , 1990, while in the presence of an inflammatory response in the genital tract, IgCC appear in the ampullae and the seminal vesicles (Campero et al 1989). It has been noted that the production of IgA and IgG in the seminal vesicles in the bull is an essential component of the local immune response (Bier et al 1977).…”

Section: Discussionsupporting

confidence: 80%

Distribution of lymphocytes, immunoglobulin-containing cells, macrophages, and dendritic cells in the accessory sex glands of rams experimentally infected with Actinobacillus seminis

et al. 2016

View full text Add to dashboard Cite

The distribution of cells involved in the immune response in accessory sex glands of rams experimentally infected with Actinobacillus seminis was studied. Twelve one-year old rams were experimentally infected by intraurethral (IU) (n=4) and intraepididymal (IE) (n=4) route, and four control (CON) animals were used. The animals were slaughtered 35 days post-inoculation, samples were taken from accessory sex glands, and bacteriology and histopathology tests were performed. The presence of CD4, CD8 and TCRγδ (WC1) lymphocytes, CD45RO cells, macrophages (CD14), dendritic cells (CD1b), IgA-, IgG-and IgM-containing cells (IgCC) was determined. Animals of the IE group developed clinical epididymitis. No lesions were seen in rams of the IU group; two of the intraepididymal inoculated CON developed small lesions in the epididymis. A. seminis isolates were achieved from 6:16 (37.5%) accessory sex glands in the IE group, but not in the IU and CON groups. In the CON group, IgA-and IgM-containing cells predominated in the bulbourethral glands and the disseminated prostate, and they were scarce or null in the vesicles and ampullae. A significant increase of IgA-, IgG-and IgM-containing cells was confirmed in the seminal vesicles, the ampullae and the bulbourethral glands in the IE group. In the IE and IU groups, an increase in CD4, CD8, WC1, CD45RO and CD14 was evidenced in the vesicles and ampullae. CD1b dendritic cells were present in the ampullae and vesicles with inflammatory processes. A. seminis triggered a local immune response in the IE and IU groups. These results indicate a different pattern of infiltrating immune cells in the accessory sex glands of infected A. seminis rams.

show abstract

Section: Discussionsupporting

confidence: 80%

Distribution of lymphocytes, immunoglobulin-containing cells, macrophages, and dendritic cells in the accessory sex glands of rams experimentally infected with Actinobacillus seminis

et al. 2016

View full text Add to dashboard Cite

show abstract

“…In addition to abortion, the disease may also cause stillbirths, birth of weak calves, placental retention, temporary or permanent infertility, perinatal mortality, and chronic or diffuse interstitial mastitis, among other problems (CORBEL, 2006;LAGE et al, 2008;XAVIER et al, 2009;POESTER et al, 2013). In males, infection can cause orchitis, epididymitis, decrease spermatozoa quality, and subfertility or sterility (CAMPERO, 1990;LAGE et al, 2008;POESTER et al, 2013). Articular lesions may occur in both sexes, typically as arthritis and bursitis (LAGE et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence and risk factors for bovine brucellosis in Minas Gerais State, Brazil

Oliveira¹,

Dorneles²,

Mota³

et al. 2016

Semin: Ciênc. Agrár.

View full text Add to dashboard Cite

This cross-sectional study was conducted in the state of Minas Gerais, Brazil in 2011 to estimate the seroprevalence of bovine brucellosis (Brucella abortus) in properties and cattle, and to identify potential infection risk factors for herds with cows of reproductive age. The state was divided into seven regions: 1. Noroeste, Norte and Nordeste; 2. Leste; 3. Central; 4. Zona da Mata; 5. Sul and Sudoeste; 6. Alto Paranaíba; and 7. Triângulo Mineiro. Random, two-stage sampling was performed of both properties and cattle from each property. Blood samples were collected from 18,990 cows of reproductive age from 2,185 properties. The serologic tests used for the detection of anti-B. abortus antibodies included the buffered, acidified plate antigen test, as a screening test, with the 2-mercaptoethanol reduction, test as a confirmatory test, as recommended by the Programa Nacional de Controle e Erradicação da Brucelose e Tuberculose Animal -PNCEBT (National Program for the Control and Eradication of Animal Brucellosis and Tuberculosis). The seroprevalence of bovine brucellosis on the surveyed properties and in cattle were 3.59% (95% confidence interval [CI]: 2.76 4.42%) and 0.81% (95% CI: 0.05-1.10%), respectively. Multiple logistic regression analysis identified the total number of cows in the property as a risk factor for the presence of anti-B. abortus antibodies, with an odds ratio (OR) of 1.93 (95% CI: 1.12 -3.34) for herds with 30 to 210 cows and 7.81 (95% CI: 3.72-16.38) for those with more than 210 cows, relative to the risk in herds with less than 30 cows, the base category. While these results demonstrate a significant reduction in the prevalence of properties with bovine brucellosis, the disease is still present in Minas Gerais, with properties with higher numbers of cows at higher risk for infection. ResumoUm estudo transversal foi realizado no estado de Minas Gerais, Brasil, em 2011, para estimar a soroprevalência da brucelose bovina (Brucella abortus) em propriedades e bovinos e identificar possíveis fatores de risco para a infecção nos rebanhos com fêmeas em idade reprodutiva. O estado foi regionalizado em sete estratos: 1. Regiões Noroeste, Norte e Nordeste; 2. Região Leste; 3. Região Central; 4. Região da Zona da Mata; 5. Regiões Sul e Sudoeste; 6. Região do Alto Paranaíba; 7. Região do Triângulo Mineiro, e a amostragem foi aleatória em dois estágios: de propriedades e, posteriormente, de bovinos em cada uma das mesmas. Foram coletadas amostras de sangue de 18.990 fêmeas em idade reprodutiva em 2.185 propriedades. Os testes sorológicos utilizados na detecção de anticorpos contra B. abortus foram o teste do antígeno acidificado tamponado e, como confirmatório, o teste de redução do 2-Mercaptoetanol, rotina preconizada pelo Programa Nacional de Controle e Erradicação da Brucelose e Tuberculose Animal (PNCEBT). O resultado da soroprevalência da brucelose bovina em propriedades e animais foi de 3.59% (IC 95%: 2,76-4,42%) e 0,81% (IC 95%: 0,05-1,10%), respectivamente. A análise de regressão logíst...

show abstract

“…Semen from sero-positive bulls has been found to contain B. melitensis [75]. In an experimental study of mature bulls, inoculation with B. abortus vaccine strain 19 led to the persistent shedding of the this bacteria in semen [76]. …”

Section: Pathogenesis and Clinical Presentation In Animalsmentioning

confidence: 99%

Comparisons of brucellosis between human and veterinary medicine

Hull

Schumaker

2018

Infection Ecology & Epidemiology

152

129

View full text Add to dashboard Cite

Brucellosis is the world’s most widespread zoonosis, but also ranks as one of the seven most neglected diseases, according to the World Health Organization. Additionally, it is recognized as the world’s most common laboratory-acquired infection. There are a reported 500,000 incident cases of human brucellosis per year. However, true incidence is estimated to be 5,000,000 to 12,500,000 cases annually. Once diagnosed, focus is directed at treating individual patients with antibiotic regimes, yet overall neglecting the animal reservoir of disease. Countries with the highest incidence of human brucellosis are Syria (1,603.4 cases per 1,000,000 individuals), Mongolia (391.0), and Tajikistan (211.9). Surveillance on animal populations is lacking in many developed and developing countries. According to the World Animal Health Information Database, Mexico had the largest number of reported outbreaks, 5,514 in 2014. Mexico is followed by China (2,138), Greece (1,268), and Brazil (1,142). The majority of these outbreaks is Brucella abortus, the etiologic agent of bovine brucellosis. Brucellosis is an ancient disease that still plagues the world. There are still knowledge gaps and a need for better diagnostics and vaccines to make inroads towards control and eradication.

show abstract

Immunopathology of experimental Brucella abortus strain 19 infection of the genitalia of bulls

Cited by 23 publications

References 18 publications

Distribution of lymphocytes, immunoglobulin-containing cells, macrophages, and dendritic cells in the accessory sex glands of rams experimentally infected with Actinobacillus seminis

Distribution of lymphocytes, immunoglobulin-containing cells, macrophages, and dendritic cells in the accessory sex glands of rams experimentally infected with Actinobacillus seminis

Seroprevalence and risk factors for bovine brucellosis in Minas Gerais State, Brazil

Comparisons of brucellosis between human and veterinary medicine

Contact Info

Product

Resources

About