Immunoperoxidase Localization of Prolactin in Syncytio‐trophoblast Cells of Normal Pregnancy, Aborted Pregnancy, Hydatidiform Mole and Choriocarcinoma

Abstract

In this study the histochemical immunoperoxidase method was adopted t o clarify the localization of prolactin (PRL) in the decidua and villus of normal, aborted, and molar pregnancy and choriocarcinoma. After evacuation and curettage or operative resection, tissue specimens were fixed in formalin solution and emb,edded in paraffin. Some frozen sections were made after fixation by Zamboni's solution. The indirect immunoperoxidase method was used. Anti-PRL antibody was donated by NIAMDD and was used after absorption by hPL. Results showed that: (1) In normal pregnancy, PRL is located in syncytiotrophoblast cells. (2) In spontaneous abortion, PRL is detected in syncytiotrophoblast cells, despite fetal death, provided that hCG continues to be produced. (3) In molar pregnancy, PRL is localized in syncytie trophoblast cells, even those which penetrate into the decidua. (4) In choriocarcinoma, PRL is located ih the cancer cells.We concluded that syncytiotrophoblast cells are the main site of PRL as long as trophoblast cells are alive and active.

Immunohistological Description of Nongestational Ovarian Choriocarcinoma in Two Female Mice With Conditional Loss of Trp53 Driven by the Tie2 Promoter

“…Thus, as a second option, we applied FBP and PRL, markers known to be constitutively expressed in normal placental tissues and in choriocarcinomas from both humans and rodents. 9,13,16 NGCO immunophenotype was further characterized by lack of CD31 expression, thus confirming that blood filled lacunae within the mass were lined by neoplastic trophoblastic giant cells rather than by endothelial cells. This peculiar feature, known as ''vasculogenic mimicry,'' has been described in choriocarcinomas.…”