Immunoperoxidase Technique on Paraffin Sections Fails to Demonstrate Immunoglobulin Deposition in Cutaneous Lesions from Patients with Dermatomyositis

Roebker, James; Case, J.; Lear, Sheron C.; Hodge, Steven J.; Callen, Jeffrey P.

doi:10.1097/00000372-198808000-00003

Cited by 7 publications

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“…On the other hand, the use of immunohistochemistry on paraffin‐embedded tissue to show complement and immunoglobulin deposition in the skin is not well established. Only a few studies report the use of immunohistochemistry on formalin‐fixed tissue in the assessment of inflammatory skin diseases 10–12 demonstrating a comparatively high number of false‐negative results. Immunohistochemical detection of C3d and C4d deposition in inflammatory skin diseases has recently been described as a valuable diagnostic tool by Magro et al 1 We analyzed the expression of C3d in formalin‐fixed tissue in autoimmune bullous disorders as a possible substitute for DIF.…”

Section: Discussionmentioning

confidence: 99%

C3d immunohistochemistry on formalin‐fixed tissue is a valuable tool in the diagnosis of bullous pemphigoid of the skin

et al. 2010

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Section: Discussionmentioning

confidence: 99%

C3d immunohistochemistry on formalin‐fixed tissue is a valuable tool in the diagnosis of bullous pemphigoid of the skin

et al. 2010

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“…There are a few earlier articles describing IH and immunofluorescent assessment of immunoglobulins on paraffin-embedded tissue as an alternative to DIF on fresh tissue. [42][43][44] The last article published on the topic was in 1988. There was a high incidence of false-negative results.…”

Section: Discussionmentioning

confidence: 99%

The use of C3d and C4d immunohistochemistry on formalin-fixed tissue as a diagnostic adjunct in the assessment of inflammatory skin disease

Magro

Dyrsen

2008

Journal of the American Academy of Dermatology

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“…La prédominance de l'érythroedeme péri-orbitaire (66 %) au cours de la dermatomyosite est également rapportée dans les autres séries de la littérature [16,[22][23][24]. De même, les papules de Gottron décrites dans 30% des cas dans la littérature [25], étaient retrouvées dans 14,3% des cas dans notre étude.…”

Section: Discussionunclassified

Untitled

2022

Our Dermatol Online

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Introduction:The general objective was to determine the epidemiological, clinical, paraclinical, therapeutic and evolutionary aspects of connectivitis in the dermatology department of the CHU Aristide Le Dantec. Patients and methods: This was a retrospective and descriptive study conducted in the Dermatology Department of the University Hospital Center (UHC) Aristide Le Dantec (HALD) of Dakar between January 2009 and December 2019. Their diagnoses were retained according to the international criteria validated in the course of connectivites Results: We identified 290 cases of connectivitis. Systemic scleroderma was the most frequent in 34.42% (n=94), followed by systemic lupus in 25.86% (n=75), mixed connectivities in 22,06% (n=64) and dermatomyositis in 19,32% (n=56). Cancer was associated with dermatomyositis in 11 cases and with systemic scleroderma in 3 cases. The mean age of our patients was 38.23 years. The sex ratio was 4.2 and the mean duration of evolution was 25 months. Dermatological manifestations were constant, found in all patients. In systemic lupus, they were dominated by discoid lupus lesions found in 40% and hypochromic spots (86%) in systemic scleroderma. In dermatomyositis, skin lesions were dominated by periorbital erythroedema (66.07%). Joint manifestations were found in 56.89% of cases (n= 165), muscular manifestations in 41.37% of cases (n= 120) and pleuropulmonary manifestations in 31.3% of cases (n=91). Neuropsychiatric manifestations were noted in 5.17% of cases (n=15). All our patients were treated with corticosteroid therapy and 12.06% of them had received immunosuppressive treatment, the most commonly used molecules being methotrexate (3.44%) and cyclophosphamide (8.27%). Hydroxychloroquine was prescribed in 59.31% of patients, while D-penicillamine was prescribed in 42.06% of patients, 100% of whom were treated for systemic scleroderma. The main complications encountered were infectious complications in 83 cases (28.6%). Visceral complications, a type of visceral insufficiency, were noted in 4.13% of cases. Conclusion: Our study demonstrates the frequency of connectivitis in the dermatology department. Its particularity was the predominance of systemic scleroderma over lupus which was classically the first connectivite in dermatology services. The delay in diagnosis noted is correlated with visceral attacks; hence the importance of a better knowledge of these affections for an early management.

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Immunoperoxidase Technique on Paraffin Sections Fails to Demonstrate Immunoglobulin Deposition in Cutaneous Lesions from Patients with Dermatomyositis

Cited by 7 publications

References 0 publications

C3d immunohistochemistry on formalin‐fixed tissue is a valuable tool in the diagnosis of bullous pemphigoid of the skin

C3d immunohistochemistry on formalin‐fixed tissue is a valuable tool in the diagnosis of bullous pemphigoid of the skin

The use of C3d and C4d immunohistochemistry on formalin-fixed tissue as a diagnostic adjunct in the assessment of inflammatory skin disease

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