Immunophenotype Lymphocyte of Peripheral Blood in Patients with Psoriasis

Abstract

Introduction:Regulatory T cells (Treg) play a central role in the immunopathogenesis of psoriasis. Immunoregulatory T cells (Tregs) are involved in important homeostatic mechanism for maintaining tolerance and preventing autoimmunity, and autoimmune diseases. The aim of this study was to examine the role of Tregs cells in the pathogenesis of psoriasis, and determine the range value for Treg cells (CD4+ CD25+) in the peripheral blood of patients with psoriasis compared to the severity of disease.Material and methods:The study included 51 patients diagnosed with psoriasis and 25 healthy individuals. Phenotype profile of peripheral blood lymphocytes was determined by flow cytometry, and assessment of severity of disease was determined on the basis of PASI score (e.g. Psoriasis Area and Severity Index).Results:Proportion of CD4+CD25+T cells in the control group was significantly higher than in the patients with psoriasis [6,4% ±(5,4-7,6) vs. 4,1% (3,1 -5,8)–Mann–Whitney U test, p <0.001]. In the present study we did not find a statistically significant correlation between the levels of CD4+CD25+cells, in patients with psoriasis, compared to the severity of disease–PASI. (i.e. Pearson correlation, r = 0.197, p = 0.194).Conclusion:The stratification of patients, according to the severity of the clinical course was not possible on the basis of Treg cells’ level. ROC curve analysis of the optimal cutoff (PASI=10) and the CD4+CD25+, which distinguishes between patients and healthy individuals was 5% of CD4+CD25+ of the total number of CD4+ lymphocytes with specificity of 69% and sensitivity of 84%.

The role of regulatory T cells and anti-inflammatory cytokines in psoriasis

“…The predominance of Th1/IFN-γ and proinflammatory cytokines develops in the chronic phase (2)(3)(4). An essential factor of pathogenesis is the dysfunction of regulatory lymphocytes (Treg), which are involved in homeostasis mechanisms to maintain tolerance and prevent autoimmune disorders (5).…”

Kaempferol attenuates imiquimod-induced psoriatic skin inflammation in a mouse model

“…Extensive studies have shown that CD4 + Foxp3 + T regs and CD4 + T cells play a crucial but opposite role in the context of psoriasis . We therefore explored whether kaempferol would exert its therapeutic effects on psoriasis through up‐regulating CD4 + FoxP3 + T regs .…”

Interleukin 10 promotor gene polymorphism in the pathogenesis of psoriasis

“…Treg cells maintain immunological homeostasis and also prevent autoimmunity against self-antigens. The pathogenesis of psoriasis is mainly due to the increased expression of Th1 and Th17 cytokines and decreased expression of Th2 and Treg cytokines (20)(21)(22). Several studies have reported that the number and activity of Treg cells are decreased in psoriasis (23).…”

“…Studies have reported that hyperproliferation of psoriatic lesions is a result of the abnormal activity of Treg cells in the sera and skin lesions of psoriatic patients. Treg cells have been found to have an important role in the inflammation of psoriatic skin lesions (22,(26)(27)(28).…”