Immunophenotypic analysis of T-cell acute lymphoblastic leukaemia in Madras, India

Rajalekshmy, Kamalalayam Raghavan; Abitha, Arcot Radhakrishnan; Pramila, Radhakrishnan; Gnanasagar, Tenali; Shanta, V.

doi:10.1016/s0145-2126(96)00092-6

Cited by 17 publications

(15 citation statements)

References 24 publications

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“…CD5 also came out as a highly sensitive marker (positive in 84.6% of the cases). The high sensitivity of these markers is in accordance with the previous reports and is thus the most commonly used antigens for T-ALL diagnosis [28]. Positivity of CD3 was 38.5% and like some previous studies was not confirmed as the most sensitive marker, which is limited by the fact that it is uncommonly expressed on the surface of T-ALL and is almost always present abundantly in the cytoplasm of these cells [13].…”

Section: Immunophenotypic Criteria Of T-allsupporting

confidence: 87%

Flowcytometric Immunophenotypic Profile of Acute Leukemia: Mansoura Experience

Salem

El-Aziz

2011

Indian J Hematol Blood Transfus

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Acute leukemia (AL) displays characteristic patterns of antigen expression, which facilitate their identification and proper classification. The purpose of this study is to evaluate the diagnostic usefulness of commonly used immune-markers for immunophenotyping of AL and to define the best immune-markers to be used for proper diagnosis and classification of AL. Besides, to recognize the frequency of different AL subtypes and the antigen expression profile in our Egyptian patients. We retrospectively analyzed the immunophenotypic data of 164 de novo AL patients from our institution during 2009 and 2010. Among these patients, 68.9% were classified as acute myeloblastic leukemia (AML) while 31.1% classified as acute lymphoblastic leukemia (ALL). The commonest FAB subtype in AML group was AML-M4/5 (34.5%) which may differ from most published data. As regard ALL, there were 74.5% with B-ALL and 25.5% with T-ALL. It was found that combined use of HLADR and CD34 was much more helpful in distinguishing APL from non-APL AML than either of these antigens alone. It was found that cCD79a and CD19 were the most sensitive marker for B-ALL while cCD3, CD7 and CD5 were the most sensitive antigens for T-ALL. Our analysis of AL phenotypes proved that employed antibody panels are adequate for proper diagnosis and classification of AL. Flowcytometry was found to be especially useful in the identification of AML-M0 and differentiation of APL from non-APL AML. Immunophenotyping results and FAB classification of our AL patients were comparable to internationally published studies apart from predominance of AML-M4/5 and more frequent APL.

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Section: Immunophenotypic Criteria Of T-allsupporting

confidence: 87%

Flowcytometric Immunophenotypic Profile of Acute Leukemia: Mansoura Experience

Salem

El-Aziz

2011

Indian J Hematol Blood Transfus

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“…This may be due to the deletion of TCRd and commitment to the TCR ab rearrangement. In an earlier report from our Institute conducted with 285 T-ALL cases showed that CD7, CD5, and CD2 were commonly expressed [1]. A study conducted in Indian T-ALL patients reported that Vd1-Jd1 was predominantly associated with CD2+, CD7+, CD4+, and TCRgd+ [8].…”

Section: Discussionmentioning

confidence: 87%

“…The percentage of T-ALL in India is very high (20-44%) when compared to the Western countries (10-20%). The percentage of T-ALL is 20.7% in West India, 31.8% in North India, and is highest (43.1%) in South India [1,2]. According to the Madras Metropolitan Tumor Registry, Chennai, South India, the age standardized rate of childhood acute lymphoblastic leukemia (ALL) during 1993-1997 is 4.2 out of 100,000 in boys and 2.3 out of 100,000 in girls [3].…”

Section: Introductionmentioning

confidence: 99%

T‐cell receptor gamma and delta gene rearrangements and junctional region characteristics in south Indian patients with T‐cell acute lymphoblastic leukemia

et al. 2006

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Clonal T-cell receptor (TCR) gamma and delta gene rearrangements were studied in 40 T-ALL cases (pediatrics, 29; adults, 11) using PCR with homo-heteroduplex analysis. At least one clonal TCRG or TCRD rearrangement was detected in 34 (85%) cases. TCR gamma (TCRG) rearrangement was detected in 25 (62.5%) cases that included 16 (55%) pediatrics and 9 (81.8%) adults. TCR delta (TCRD) rearrangement was detected in 14/40 (35%) cases, which included 12 (41%) pediatrics and 2 (18%) adults. The frequency of VgI-Jg1.3/2.3 was significantly more in adults than pediatrics (81.8% vs. 41.3%, P = 0.02). In TCRD, Vd1-Jd1 was rearranged in 10 (25%) cases. The surface membrane CD3 positive cases are significantly associated with absence of TCRD rearrangements (surface membrane CD3+ TCRd-84% vs. surface membrane CD3-TCRd-48%, P value = 0.03). Junctional region sequence analyzed with 10 cases each, of TCRG and TCRD, revealed an average junctional region of 7.4 nucleotides (range 2-18 nucleotides) in TCRG and 27 nucleotides (range 14-42 nucleotides) in TCRDcomplete rearrangements. In TCRG, trimming at the ends of Vg and Jg germline nucleotides resulted in deletion, on an average of 9.2 nucleotides. In TCRD, deletion of nucleotides of the Vd and Jd gene segments on an average was 3.5 nucleotides. The junctional region of TCRD is more diverse than TCRG; nevertheless, the frequency of TCRG was more than that of TCRD and hence we rely more on TCRG clonal markers to quantitate the minimal residual disease in T-ALL. Am. J. Hematol. 82:215-221, 2007. V V C 2006 Wiley-Liss, Inc.

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“…[4][5][6] A comparison of relative frequencies of major immunosubtypes shows striking differences between developed and developing countries. [7][8][9][10][11][12][13][14][15][16][17][18] Developed countries showed predominance in common acute lymphoblastic leukemia (C-ALL) cases, whereas in developing countries a relative predominance in T-cell acute lymphoblastic leukemia (T-ALL) and a selective deficit in C-ALL were reported. Socioeconomic and environmental factors are considered to be important determinant of major immunophenotype in pediatric ALL cases in a particular geographic setting.…”

Section: Childhood Acute Lymphoblastic Leukemiamentioning

confidence: 99%