Immunophenotyping: Analytical approaches and role in preclinical development of nanomedicines

Newton, Hannah S.; Dobrovolskaia, Marina A.

doi:10.1016/j.addr.2022.114281

Cited by 14 publications

(13 citation statements)

References 83 publications

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“…The level of IgE-mediated activation can be revealed by in vitro studies by flow cytometry after staining the cells with panels of specific fluorochrome-antibody conjugates. DURAClone is one such kit containing dry antibodies against CD3, CD45, CD63, CD203c and CD294 [ 141 , 142 , 143 ].…”

Section: Clinical Presentation and Diagnostic Methods In Hsrsmentioning

confidence: 99%

Allergy in Cancer Care: Antineoplastic Therapy-Induced Hypersensitivity Reactions

Gălățeanu

Puscasu

Tîrcol

et al. 2023

IJMS

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As the backbone of oncological treatments, systemic chemotherapy is still one of the main pawns in cancer care, alone or in combination with newer targeted agents. All chemotherapy agents can be associated with a type of adverse event called an infusion reaction, which can be characterized as unpredictable, non-dose related, and unexplained by the cytotoxic profile of the drug. For some of these events, a certain immunological mechanism can be identified by blood or skin testing. In this case, we can speak of true hypersensitivity reactions that occur as a response to an antigen/allergen. The current work summarizes the main antineoplastic therapy agents and their susceptibility to induce hypersensitivity reactions and also includes a review of clinical presentation, diagnostic methods in hypersensitivity reactions, and perspectives to overcome these negative events in the treatment of patients suffering from various types of cancer.

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Section: Clinical Presentation and Diagnostic Methods In Hsrsmentioning

confidence: 99%

Allergy in Cancer Care: Antineoplastic Therapy-Induced Hypersensitivity Reactions

Gălățeanu

Puscasu

Tîrcol

et al. 2023

IJMS

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“…In turn, immune cells are stimulated by proinflammatory cytokines, leading to immune dysregulation in patients. Nanomaterials are widely studied in biology and medicine due to their excellent chemical and physical properties, such as intervening in the expression and signaling pathways of immune cells through their intrinsic properties or loaded drugs 171 , 172 , 173 . Therefore, the introduction of NPs to target immune cells may be another inspired strategy for COVID-19 174 , 175 , such as the most important macrophages and neutrophils involved in the immune response activated by SARS-CoV-2.…”

Section: Nanotechnology-related Strategies Ameliorate Covid-19mentioning

confidence: 99%

Regulating the microenvironment with nanomaterials: Potential strategies to ameliorate COVID-19

Liu

Han

Jin

et al. 2023

Acta Pharmaceutica Sinica B

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“…Lymphoid-derived cells include B cells, T cells, natural killer (NK) cells, and invariant natural killer T (iNK-T) cells, whereas myeloid-derived cells include macrophages, neutrophils, monocytes, and dendritic cells [16,17] (Table 1). Bone marrow and spleen are the main tissues of choice for studying immune cell populations in immunophenotyping procedures using rodent models and human peripheral blood [24]. However, when doing children's diagnosis, it is suggested to use bone marrow samples for the first test [25].…”

Section: Introductionmentioning

confidence: 99%

“…In addition to classical multicolour flow cytometry, different cytometric methods have been developed for immunophenotyping, each of which has been designed for different purposes including hyperspectral flow cytometry, imaging flow cytometry (IFC), mass cytometry, and imaging mass cytometry (IMC) (Table 2) [24,28]. Immunophenotyping is commonly used to diagnose specific types of cancers, such as different kinds of lymphoma and leukemia [36].…”

Section: Introductionmentioning

confidence: 99%

Immunophenotyping to improve the mechanistic understanding of idiosyncratic drug-induced liver injury: clinical implications and future directions

et al. 2023

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The late event onset of a fraction of idiosyncratic drug-induced liver injury (DILI) cases and the link observed by genome-wide association studies (GWASs) of certain human leucocyte antigen (HLA) alleles with DILI due to specific drugs support the crucial role of the immune system (both innate and adaptive) in the pathogenesis of DILI. Recent advances in both flow and mass cytometry have allowed the profiling of all major immune cell types in a given sample. Therefore, determining the lymphocyte populations in samples from patients with DILI would facilitate the development of specific biomarkers for DILI diagnosis and prognosis. To date, a few studies have explored the immune landscape in DILI. In a recent study of leukocyte immunophenotyping using flow cytometry from the Spanish DILI Registry, an important role of adaptive immune response in DILI is suggested. DILI patients had significantly higher levels of T helper 1 (Th1) cells and activated helper and cytotoxic T cells than healthy controls. Furthermore, the increased expression of negative immune checkpoints and ligands in DILI patients could reflect a restoration of the immune homeostasis. Differences in the profile of cytokines in DILI patients from the Drug-Induced Liver Injury Network (DILIN) also suggest an involvement of both innate and adaptive immune systems in DILI development and prognosis. Moreover, several studies based on immunophenotyping of liver infiltrates showed a distinctive pattern of cellular infiltrates in patients with immune checkpoint inhibitors (ICIs)-DILI, with lower levels of plasma cells, CD20+ B cells and CD4+ T cells than in autoimmune hepatitis (AIH) patients. These pioneering studies highlight the importance of immunophenotyping for the mechanistic understanding of DILI. In this review, available data on immunophenotyping in DILI are gathered, and the potential clinical applications of cutting-edge, novel immunophenotyping techniques are discussed.

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Immunophenotyping: Analytical approaches and role in preclinical development of nanomedicines

Cited by 14 publications

References 83 publications

Allergy in Cancer Care: Antineoplastic Therapy-Induced Hypersensitivity Reactions

Allergy in Cancer Care: Antineoplastic Therapy-Induced Hypersensitivity Reactions

Regulating the microenvironment with nanomaterials: Potential strategies to ameliorate COVID-19

Immunophenotyping to improve the mechanistic understanding of idiosyncratic drug-induced liver injury: clinical implications and future directions

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