Immunophenotyping of hematopoietic progenitor cells: Comparison between cord blood and adult mobilized blood grafts

Azouna, Nesrine Ben; Berraeis, Lamia; Regaya, Zohra; Jenhani, F.

doi:10.4252/wjsc.v3.i11.104

Cited by 11 publications

(10 citation statements)

References 31 publications

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“…Previous reports 14 suggested that maternal age is not significantly related to the umbilical cord blood and CD34 + cell count, as well as the number of NRBC’s in the umbilical cord blood. However, a previous report 28 showed that women >25 years old carry more TNCs, and younger maternal age was associated with a higher CD34 + cell concentration. 16 This observation may serve as a parameter during the selection and evaluation of the usefulness of a specific umbilical cord blood specimen.…”

Section: Discussionmentioning

confidence: 77%

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Impact of maternal and neonatal factors on parameters of hematopoietic potential in umbilical cord blood

Al-Deghaither

2015

SMJ

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Objectives:To determine characteristics of laboratory parameters of hematopoietic potential in umbilical cord blood and their association with maternal and neonatal factors.Methods:This prospective analysis was performed on 206 umbilical cord blood donations (50-200 ml) from King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia between January and September 2014. Samples were processed and analyzed for total nucleated cells (TNC’s), cluster designation (CD)45+, CD34+ counts, nucleated red blood cells (NRBCs) count, and viability testing.Results:Most of the study participants (63.6%) were on their first 3 deliveries and from women with age between 17 and 30 years (80.6%). The donated volume was 50.4-192.4 ml, TNCs ranged from 500.2×106 to 9430.3 ×106 cells, and CD34+ cells ranged from 1.25×106 to 12.82×106/unit. The volume was positively affected by bigger birth weight of the baby (p<0.0001), larger placenta (p=0.001), TNCs (p<0.0001), CD34+ (p<0.0001), NRBCs (p<0.0001), and viability (p=0.002). There were no statistically significant differences between baby boys and girls for laboratory variables.Conclusion:In the selection and identification of a possible donor of umbilical cord blood, several maternal and neonatal factors should be considered, as younger maternal age, neonatal birth weight >3300 grams, larger placental size, and first or second-born babies, were shown to be associated with higher TNCs, CD34+, CD45+, NRBCs, and viability.

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Section: Discussionmentioning

confidence: 77%

“…In addition, the antigen CD45 is one of the most important factors in the detection of CD34 + by different qualitative and quantitative protocols. 28 This is why it was included in our study panels.…”

Section: Discussionmentioning

confidence: 99%

Impact of maternal and neonatal factors on parameters of hematopoietic potential in umbilical cord blood

Al-Deghaither

2015

SMJ

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“…In a prior study, Azouna et al found that the number of CD34 + cells was higher in the cord blood of neonates with higher birth weight (>3.5 kg) and born to older mothers [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

Increased Proportion of Hematopoietic Stem and Progenitor Cell Population in Cord Blood of Neonates Born to Mothers with Gestational Diabetes Mellitus

Hadarits

Zóka

Barna

et al. 2016

Stem Cells and Development

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We assessed the hematopoietic stem and progenitor cell (HSPC) population in the cord blood of neonates born to mothers with gestational diabetes mellitus (GDM) in a hypothesis generating pilot study, due to that, neonatal polycythemia may be the consequence of GDM pregnancy. Forty-five pregnant women with GDM (last trimester mean HbA1C = 33.9 mmol/mol) and 42 (nondiabetic) control pregnant women were enrolled after their routine 75 g oral glucose tolerance test (OGTT) between the 24th and 28th gestational week (with expected differences in their mean routine clinical characteristics: plasma glucose at OGTT: 0′ = 5.07 vs. 4.62 mM, 120′ = 8.9 vs. 5.76 mM, age = 35.07 vs. 31.66 years, prepregnancy body mass index = 27.9 vs. 23.9 kg/m2, GDM vs. control, respectively) on a voluntary basis after signing the informed consent. EDTA-treated cord blood samples were analyzed by flow cytometry and the software Kaluza1.2 using CD45 and CD34-specific fluorescent antibodies to identify the HSPC population (CD34+ cells within the CD45dim blast gate). The proportion of CD34+CD45dim HSPCs among the nucleated cells was significantly (P < 0.05, statistical power = 60.8%) higher in the cord blood samples of neonates born to mothers with GDM (median 0.38%) compared to neonates born to nondiabetic mothers (median 0.32%) and according to treatment types (P < 0.05) median: control 0.32%, GDM-diet only 0.37%, GDM-on insulin 0.45%; control versus GDM on insulin (P < 0.05). The increased proportion of circulating CD34+CD45dim cells in the cord blood may possibly be related to altered fetal stem cell mobilization in GDM pregnancy, yet these results should be interpreted only as preliminary due to the small sample sizes.

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“…hPL has been explored as a substitute to FBS for cell culture and found to have advantages beyond that of simply eliminating xenogeneic concerns. Exposure of human mesenchymal stem cells (hMSCs) to hPL in vitro has positive effects, including increased proliferation, decreased doubling time, maintained immunophenotype and delayed senescence (Doucet et al , ; Muller et al , ; Reinisch et al , ; Schallmoser et al , ; Carrancio et al , ; Avanzini et al , ; Azouna et al , ; Flemming et al , ; Griffiths et al , ; Rubio‐Azpeitia and Andia, ).…”

Section: Introductionmentioning

confidence: 99%

Human platelet lysate supplementation of mesenchymal stromal cell delivery: issues of xenogenicity and species variability

Allen

Butts

Copland

et al. 2016

J Tissue Eng Regen Med

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Immunogenicity of fetal bovine serum (FBS) poses a problem for its use in the propagation of autologous mesenchymal stromal cells (MSCs) for cell therapy. Human platelet lysate (hPL), an enriched growth factor solution containing mitogenic and angiogenic cues, has potential utility in replacing FBS for human MSC (hMSC) delivery strategies. Despite its potentiation of hMSC number in vitro, little is known concerning its capacity to supplement implanted hMSC-seeded constructs and promote tissue regeneration in vivo. In this study, we tested the effects of incorporating hPL in cell-seeded constructs implanted subcutaneously into immunocompromised rats, investigated in vitro interactions between hPL and rat MSCs (rMSCs) and determined interspecies variability in the PL product [hPL vs rat PL (rPL)] and its effect on cultured MSCs (hPL/hMSCs vs rPL/rMSCs). The overarching aim was to determine the utility of hPL to foster MSC survival in preclinical rodent models. Exposure to hPL-supplemented media resulted in rMSC death, by a process attributable to heat-labile proteins, but not membrane attack complex formation. In the in vitro syngeneic model, the rodent product proved fundamentally distinct from the human product, with rPL having substantially lower growth factor content than hPL. Moreover, contrary to the positive effects of hPL on hMSC expansion, rPL did not reduce rMSC doubling time for the serum concentrations examined. When tested in vivo, hPL did not improve cell survival within hydrogel constructs through 2 weeks postimplantation. In summary, this study highlights the many facets of xenogenicity and interspecies variability that must be considered in the preclinical evaluation of hPL. Copyright © 2016 John Wiley & Sons, Ltd.

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Immunophenotyping of hematopoietic progenitor cells: Comparison between cord blood and adult mobilized blood grafts

Abstract: This study demonstrates that many different obstetric factors must be taken into account when processing and cryo-banking umbilical CB units for transplantation.

Cited by 11 publications

References 31 publications

Impact of maternal and neonatal factors on parameters of hematopoietic potential in umbilical cord blood

Impact of maternal and neonatal factors on parameters of hematopoietic potential in umbilical cord blood

Increased Proportion of Hematopoietic Stem and Progenitor Cell Population in Cord Blood of Neonates Born to Mothers with Gestational Diabetes Mellitus

Human platelet lysate supplementation of mesenchymal stromal cell delivery: issues of xenogenicity and species variability

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