Immunophenotyping of Non-Hodgkin's Lymphoma in Paraffin-Embedded Tissue Sections: A Comparison with Genotypic Analysis

Abstract

Several monoclonal antibodies (MoAbs) are now available for immunophenotyping non-Hodgkin's lymphomas (NHLs) in paraffin-embedded tissue sections. To determine the reliability of these reagents in predicting the genotype, 44 cases of NHL were studied with the alkaline phosphatase-anti-alkaline phosphatase technique with the use of the following MoAbs: leukocyte common antigen (CD45), Mac 387, L26, 4KB5, MB1, MB2, LN2, UCHL1, MT1, and MT2. The lineage of the neoplastic cells was determined in all cases by gene rearrangement studies for immunoglobulin heavy chain and for the T-cell receptor beta-chain. Genotypic results showed B-cell lineage in 33 cases (75%), T-cell lineage in 6 cases (14%), and mixed or undetermined lineage in 5 cases (11%). A concordance of lineage assignment by paraffin section immunophenotyping with gene rearrangement studies was observed in 37 of 39 (95%) lymphomas with an unequivocally defined genotype. MoAb L26 was the most sensitive in detecting B-cell genotype; MoAbs MT1 and UCHL1 were the most sensitive and specific, respectively, in detecting T-cell genotype. The authors conclude that lineage assignment of NHLs in paraffin sections is reflective of the corresponding genotype when an appropriate panel of MoAbs is used.

Paraffin-resistant Antigens Detectable by Antibodies L26 and Polyclonal CD3 Predict the B- or T-Cell Lineage of 95% of Diffuse Aggressive Non-Hodgkin’s Lymphomas

“…25,26 Similarly, most investigators have failed to detect L26 reactivity with T-cell neoplasms. 1415,24,27,28 However, some investigators have reported rare L26-positive T-cell lymphomas, 16,22,23,25,26 ' 29 which are thought to be derived from a small population of normal lymphocytes that co-express CD3 and small amounts of CD20. 26 These L26-positive T-cell neoplasms, which were classified morphologically as DM (5) or DL (3) lymphomas, 16,22,23,26,29 exhibited, where described, distinct membrane staining, characteristic of L26 reactivity with B-cell NHLs.…”

“…1415,24,27,28 However, some investigators have reported rare L26-positive T-cell lymphomas, 16,22,23,25,26 ' 29 which are thought to be derived from a small population of normal lymphocytes that co-express CD3 and small amounts of CD20. 26 These L26-positive T-cell neoplasms, which were classified morphologically as DM (5) or DL (3) lymphomas, 16,22,23,26,29 exhibited, where described, distinct membrane staining, characteristic of L26 reactivity with B-cell NHLs. However, at least two of these T-cell neoplasms also reacted with one or more other preferential B-cell antibodies, 16 ' 22 suggesting that these L26-positive T-cell NHLs exhibit other phenotypic characteristics of B cells than just CD20 expression.…”

Analysis of Antral Biopsy Specimens for Evidence of Acquired Mucosa-Associated Lymphoid Tissue in HIV1-Seropositive and HIV1-Negative Patients

“…-directed against the T-cell-associated CD45RO antigen (both DAKO) (12). Visualization was performed us,ng a streptavidin-biotin technique.…”

Analysis of immunoglobulin light-chain mRNA in gastric malignant lymphoma using new highly sensitive in situ hybridization method

“…Southern blotting is limited for diagnostic work because it is time-consuming and complex, and requires the use of radioactive isotopes and large amounts of DNA. In addition, it is not well suited to the study of formalin-fixed and paraffin-embedded material because of the consequent DNA degeneration [6][7][8][9]. Although immunohistologic studies for the detection of immunoglobulin light chains have been widely employed to determine monotypic restriction, the results have not been consistently successful, particularly with formalin-fixed and paraffin-embedded sections [10,11].…”