Immunophysiology of Intestinal Electrolyte Transport

Powell, Don W.

doi:10.1002/cphy.cp060425

Cited by 28 publications

(23 citation statements)

References 412 publications

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“…4, EC 50 ϭ 1 ng/ml, P Ͻ 0.01 by ANOVA), TNF-␣ (EC 50 ϭ 25 ng/ml, maximal ⌬Isc of 27.3Ϯ6.4 A/cm 2 at 18 h, P Ͻ 0.001 compared with media control). Endothelial cell exposure to hypoxia (pO 2 20 torr, for periods of 12-48 h) (9, 10) also elicited liberation of secretagogue activity (⌬Isc of 8.4Ϯ1.7, 9.8Ϯ1.6, and 10.3Ϯ1.9 A/cm 2 for periods of hypoxia of 12, 24, Figure 2. Induction of electrogenic Cl Ϫ secretion and fluid transport in LPS-activated endothelial-epithelial cocultures.…”

Section: Resultsmentioning

confidence: 99%

“…Where indicated, confluent monolayers were exposed to activating conditions (LPS, IL-1 ␣ , or TNF-␣ ) as described before (9). In subsets of experiments, serum-starved endothelia (12 h) were exposed to hypoxia (pO 2 20 mmHg) as previously reported (9,10).…”

Section: Cell Culturementioning

confidence: 99%

“…Analysis of extracted soluble supernatants from endothelia preexposed to LPS (500 ng/ml, 18 h), TNF-␣ (50 ng/ml, 18 h), IL-1␣ (30 ng/ml, 18 h), or ambient cellular hypoxia (pO 2 20 torr, 48 h) revealed soluble 6-keto-PGF 1␣ concentrations of 9.2Ϯ1.5, 13.2Ϯ3.4, 15.3Ϯ6.2, and 9.0Ϯ3.1 ng/ml, respectively (n ϭ 3 separate experiments, all P Ͻ 0.01 compared with endothelial media alone). Importantly, media derived from endothelia not exposed to activating stimuli (18-h exposure) also had measurable amounts of 6-keto-PGF 1␣ (0.22Ϯ0.03 ng/ml, P Ͻ 0.05 compared with media alone).…”

Section: Figure 5 Eds Activity Parallels Pghs-2 (Cox-2) Induction (A)mentioning

confidence: 99%

“…By secreting solutes and transporting fluid across the epithelium, epithelia are able to coordinate compositional changes of the lumenal compartment. Several stimuli, including hormones, neurotransmitters, and cytokines have been shown to directly regulate both epithelial barrier function and ion transport (1)(2)(3).…”

Section: Introductionmentioning

confidence: 99%

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Activated endothelial cells elicit paracrine induction of epithelial chloride secretion. 6-Keto-PGF1alpha is an epithelial secretagogue.

Blume

Taylor²,

Lennon³

et al. 1998

J. Clin. Invest.

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Endothelial cells play a central role in the coordination of the inflammatory response. In mucosal tissue, such as the lung and intestine, endothelia are anatomically positioned in close proximity to epithelia, providing the potential for cell-cell crosstalk. Thus, in this study endothelial-epithelial biochemical crosstalk pathways were studied using a human intestinal crypt cell line (T84) grown in noncontact coculture with human umbilical vein endothelia. Exposure of such cocultures to endothelial-specific agonists (LPS) resulted in activation of epithelial electrogenic Cl Ϫ secretion and vectorial fluid transport. Subsequent experiments revealed that in response to diverse stimuli (LPS, IL-1 ␣ , TNF-␣ , hypoxia), endothelia produce and secrete a small, stable epithelial secretagogue into conditioned media supernatants. Further experiments identified this secretagogue as 6-keto-PGF 1 ␣ , a stable hydrolysis product of prostacyclin (PGI 2 ). Results obtained with synthetic prostanoids indicated that 6-keto-PGF 1 ␣ (EC 50 ϭ 80 nM) and PGI 2 stable analogues (EC 50 ϭ 280 nM) activate the same basolaterally polarized, Ca 2 ϩ -coupled epithelial receptor. In summary, these findings reveal a previously unappreciated 6-keto-PGF 1 ␣ receptor on intestinal epithelia, the ligation of which results in activation of electrogenic Cl Ϫ secretion. In addition, these data reveal a novel action for the prostacyclin hydrolysis product 6-keto-PGF 1 ␣ and provide a potential endothelialepithelial crosstalk pathway in mucosal tissue. ( J. Clin. Invest. 1998. 102:1161-1172.)

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Section: Resultsmentioning

confidence: 99%

Section: Cell Culturementioning

confidence: 99%

Section: Figure 5 Eds Activity Parallels Pghs-2 (Cox-2) Induction (A)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Activated endothelial cells elicit paracrine induction of epithelial chloride secretion. 6-Keto-PGF1alpha is an epithelial secretagogue.

Blume

Taylor²,

Lennon³

et al. 1998

J. Clin. Invest.

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“…These are the result of the inhibition of nutrient, electrolyte, and water absorption in the IBD intestine. Immune-inflammatory mediators are elevated in the chronically inflamed intestinal mucosa, which has been shown to affect electrolyte and nutrient transport pathways in patients as well as in animal models of IBD or Crohn's disease (3,29,35).…”

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confidence: 99%