Immunoprotective behaviour of plasma-membrane-associated antigens of axenic Entamoeba histolytica

Vinayak, V. K.; Purnima,; Saxena, Akshay Kumar

doi:10.1099/00222615-24-4-297

Cited by 9 publications

(4 citation statements)

References 20 publications

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“…The augmentation of T-and Bcell mediated immune responses by liposomal entrapped CW-PPC is in accordance with earlier studies, in which liposomes have been used as adjuvant for mycobacterial antigens [16], membrane antigens of Leishmania sp. [23], and Entamoeba histolytica [24]. The adjuvant role of liposomes was further supported by the observation that animals immunized with CW-PPC alone (i.e.…”

Section: Discussionmentioning

confidence: 98%

Immunoprotective behaviour of liposome entrapped cell wall subunit of Mycobacterium tuberculosis against experimental tuberculous infection in mice

Chugh

Khuller²

1993

Eur Respir J

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We wanted to determine the immunoprotective behaviour of cell wall protein peptidoglycan complex (CW-PPC) of Mycobacterium tuberculosis H37Ra, using liposomes as adjuvant, in an experimental animal model. Immunization of mice with CW-PPC entrapped in liposomes induced both humoral response, as measured by enzyme-linked immunosorbent assay (ELISA), and cell-mediated immune responses, as seen by delayed type hypersensitivity (DTH) and leucocyte migration inhibition (LMI) techniques. Ten days after complete immunization, the animals were challenged with median lethal dose (LD50) of M. tuberculosis H37Rv. The animals exhibited significant protection, as evident by 72% survival after 30 days of infection, compared to 38% survival in control animals. Protective effect of immunization with liposome entrapped CW-PPC was further substantiated by significant decrease in the number of viable bacilli in lungs, liver and spleen of immunized animals, as compared to control animals. These results indicate that immunization with liposome-entrapped mycobacterial cell wall protein peptidoglycan complex induces protection against experimental tuberculosis.

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Section: Discussionmentioning

confidence: 98%

Immunoprotective behaviour of liposome entrapped cell wall subunit of Mycobacterium tuberculosis against experimental tuberculous infection in mice

Chugh

Khuller²

1993

Eur Respir J

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“…E. histolytica surface glycoproteins of molecular mass 20, 30, 81 and 150 kDa have been implicated as major and important components of the parasite [14]. We have earlier shown immunoregulatory functions of PM-associated antigens in experimental amoebiasis [26,29]. Nine amoebic antigens were found to be recognised by immune human sera of which four antigens of 23, 24, 37 and 90 kDa molecular mass were found to be surface-associated proteins [5].…”

Section: Discussionmentioning

confidence: 99%

“…We earlier have reported anti-plasma membrane (PM) amoebic antibodies as well as anti-PM antibody-dependent macrophage mediated cytotoxicity to E. histolytica modulating the amoebic infections in experimental hosts [21,27,29]. The immunization of the experimental host with amoebic PM proteins have also been shown to eliciate specific immune responses, resulting in protection to the experimental host upon intra-hepatic challenge with virulent E. histolytica [16,26]. The E. histolytica trophozoites have been shown to be a complex mosaic of antigens [7,12,25].…”

Section: Introductionmentioning

confidence: 99%

Immunoreactivity of Entamoeba histolytica antigens with sera from amoebic patients

Shandil

Vinayak

1990

Med Microbiol Immunol

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Immune sera from 15 patients with cured amoebic liver abscess were used to recognise the antigens of Entamoeba histolytica (HMI) by immunoblotting. The amoebic proteins most frequently recognised by sera from patients with cured amoebic liver abscess had molecular masses of 8, 13, 18, 22, 29, 38, 45, 67 and 94 kDa. Six plasma membrane-associated amoebic proteins of molecular mass 29, 38, 45-67 complex, 85 and 94 kDa were strongly recognised by such sera. Two plasma membrane-associated antigens of 108 and 129 kDa were not recognised by any sera. None of the crude or plasma membrane-associated antigens were recognised by sera from five patients of idiopathic ulcerative colitis, five patients of persistent giardiasis and five normal healthy subjects. Identification of such antigens, especially plasma membrane-associated antigens may pave a way to develop specific diagnostic and immunoprotective agents.

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“…Morbidity and mortality associated with this infection persist in many poor countries. Intact trophozoites, trophozoite lysates, specific antigens and recombinant proteins protect animals against liver abscesses (Ghadirian et al, 1980;Soong et al, 1995a, b;Vinayak et al, 1987Vinayak et al, , 1980), but we do not yet have an efficient vaccine for human amoebiasis. This is due mainly to the fact that we ignore very many of the E. histolytica protein-protein interactions and the effects they have, along with the trophozoites, on producing damage in the host.…”

Section: Introductionmentioning

confidence: 99%

Expression in fibroblasts and in live animals of Entamoeba histolytica polypeptides EhCP112 and EhADH112

et al. 2004

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EhCPADH is an immunogenic, heterodimeric protein that is formed by EhCP112 (cysteine protease) and EhADH112 (adhesin), polypeptides involved in Entamoeba histolytica's cytopathic effect, target-cell adherence and phagocytosis. The EhCPADH complex is located in the plasma membrane and cytoplasmic vacuoles. Here, the independent expression of EhCP112 and EhADH112 in fibroblasts and hamsters was analysed. Also investigated was the immunological response in animals independently inoculated with plasmid pcDNA-Ehcp112, which carries the complete cysteine protease-encoding gene, or with plasmid pcDNA-Ehadh112, which carries the C terminus of the adhesin-encoding gene, or with a mixture of both. Both proteins were expressed in the plasma membranes of the transfected fibroblasts. EhCP112 was toxic for the mammalian cells. Proteins were also independently expressed in hamsters after inoculation with the plasmids. Their expression was indirectly evaluated by the presence of antibodies in the inoculated animals. Remarkably, co-immunization of the animals with the two DNA plasmids resulted in an earlier and higher anti-E. histolytica IgG induction than immunization with separate plasmids. In contrast, the cellular immune response was not noticeably improved by the plasmid mixture. Interestingly, protection against liver abscesses was detected only in animals that received the plasmid mixture and no protection was observed in hamsters independently inoculated with plasmid pcDNA-Ehcp112 or pcDNA-Ehadh112.

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Immunoprotective behaviour of plasma-membrane-associated antigens of axenic Entamoeba histolytica

Cited by 9 publications

References 20 publications

Immunoprotective behaviour of liposome entrapped cell wall subunit of Mycobacterium tuberculosis against experimental tuberculous infection in mice

Immunoprotective behaviour of liposome entrapped cell wall subunit of Mycobacterium tuberculosis against experimental tuberculous infection in mice

Immunoreactivity of Entamoeba histolytica antigens with sera from amoebic patients

Expression in fibroblasts and in live animals of Entamoeba histolytica polypeptides EhCP112 and EhADH112

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