Immunoproteomic analysis of the secretome of bovine-adapted strains of Staphylococcus aureus demonstrates a strain-specific humoral response

Drumm, Shauna D.; Cormican, Paul; O'Boyle, Padraic; Owens, Rebecca A.; Mitchell, Joan S.; Keane, Orla M.

doi:10.1016/j.vetimm.2022.110428

Cited by 2 publications

(4 citation statements)

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“…Previous studies have shown that IgG, IgM and IgA isoforms of antibodies secreted by B cells are present in the peripheral blood of dairy cows, among which IgG and IgM contents were abundant, while IgA contents were scarce ( Enger et al, 2021 ). As the staple antibody against bacterial cow mastitis, IgG is often used as a diagnostic marker due to its prolonged expression and high sensitivity ( Bourry and Poutrel, 1996 ; Wellnitz et al, 2013 ; Wall et al, 2016 ; Drumm et al, 2022 ; Khatun et al, 2022 ). The IgM normally precedes IgG antibodies and appears temporarily in an acute infection and functioning in the primary antibody-mediated immune response ( Wagner et al, 2008 ).…”

Section: Discussionmentioning

confidence: 99%

Assessment of adaptive immune responses of dairy cows with Burkholderia contaminans-induced mastitis

Zhang

Yao²,

Yang³

et al. 2023

Front. Microbiol.

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Burkholderia contaminans, an emerging pathogen related to cystic fibrosis, is known to cause potentially fatal infections in humans and ruminants, especially in immunocompromised individuals. However, the immune responses in cows following its infection have not been fully elucidated. In this study, T- and B-lymphocytes-mediated immune responses were evaluated in 15 B. contaminans-induced mastitis cows and 15 healthy cows with multi-parameter flow cytometry. The results showed that infection with B. contaminans was associated with a significant decrease in the number and percentage of B lymphocytes but with a significant increase in the proportion of IgG+CD27+ B lymphocytes. This indicated that humoral immune response may not be adequate to fight intracellular infection, which could contribute to the persistent bacterial infection. In addition, B. contaminans infection induced significant increase of γδ T cells and double positive (DP) CD4+CD8+ T cells but not CD4+ or CD8+ (single positive) T cells in blood. Phenotypic analysis showed that the percentages of activated WC1+ γδ T cells in peripheral blood were increased in the B. contaminans infected cows. Interestingly, intracellular cytokine staining showed that cattle naturally infected with B. contaminans exhibited multifunctional TNF-α+IFN-γ+IL-2+B. contaminans-specific DP T cells. Our results, for the first time, revealed a potential role of IgG+CD27+ B cells, CD4+CD8+ T cells and WC1+ γδ T cells in the defense of B. contaminans-induced mastitis in cows.

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Section: Discussionmentioning

confidence: 99%

Assessment of adaptive immune responses of dairy cows with Burkholderia contaminans-induced mastitis

Zhang

Yao²,

Yang³

et al. 2023

Front. Microbiol.

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“…Therefore, different strains may have different mechanisms by which they infect or colonise the mammary gland and cause disease. In a previous study we demonstrated that MOK124 produced more toxins in vitro than MOK023 and the secreted proteins from MOK124 against which a serum IgG response was targeted during bovine IMI included a number of toxins [ 28 ]. The results from this study suggest that the serum IgG response to MOK023 targets many high molecular weight proteins, most likely CWA proteins, further supporting the hypothesis that these strains have contrasting modes of pathogenicity.…”

Section: Discussionmentioning

confidence: 99%

“…Quality control checks were carried out on both forward and reverse reads using the FastQC software [ 27 ]. Draft genomes were assembled and annotated as previously described [ 28 ]. For each putative protein, subcellular location was predicted using PSORTb v3.0 [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

“…Protein samples were separated by either one-dimensional (1D) or two-dimensional (2D) SDS-PAGE, as previously described [ 28 ]. Briefly, protein samples were prepared for 1D electrophoresis by adding LDS Sample Buffer (NuPAGE, Thermo Scientific, Waltham, USA) to a final concentration of 1 X, boiled for 10 min and separated on 8–12% SDS-PAGE gels using the Mini-PROTEAN Tetra Cell (Bio-Rad, Hercules, USA).…”

Section: Methodsmentioning

confidence: 99%

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Immunoproteomic analysis of the serum IgG response to cell wall-associated proteins of Staphylococcus aureus strains belonging to CC97 and CC151

Drumm,

Cormican,

Owens

et al. 2023

Vet Res

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CC97 and CC151 are two of the most common Staphylococcus aureus lineages associated with bovine intramammary infection. The genotype of the infecting S. aureus strain influences virulence and the progression of intramammary disease. Strains from CC97 and CC151 encode a distinct array of virulence factors. Identification of proteins elaborated in vivo will provide insights into the molecular mechanism of pathogenesis of these lineages, as well as facilitating the development of tailored treatments and pan-lineage vaccines and diagnostics. The repertoire of genes encoding cell wall-anchored (CWA) proteins was identified for S. aureus strains MOK023 (CC97) and MOK124 (CC151); MOK023 encoded more CWA proteins than MOK124. Serum collected during an in vivo challenge trial was used to investigate whether the humoral response to cell wall proteins was strain-specific. Immunoproteomic analysis demonstrated that the humoral response in MOK023-infected cows predominantly targeted high molecular weight proteins while the response in MOK124-infected cows targeted medium or low molecular weight proteins. Antigenic proteins were identified by two-dimensional serum blotting followed by mass spectometry-based identification of immunoreactive spots, with putative antigens subsequently validated. The CWA proteins ClfB, SdrE/Bbp and IsdA were identified as immunogenic regardless of the infecting strain. In addition, a number of putative strain-specific imunogens were identified. The variation in antigens produced by different strains may indicate that these strains have different strategies for exploiting the intramammary niche. Such variation should be considered when developing novel control strategies including vaccines, therapeutics and diagnostics.

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Immunoproteomic analysis of the secretome of bovine-adapted strains of Staphylococcus aureus demonstrates a strain-specific humoral response

Cited by 2 publications

References 48 publications

Assessment of adaptive immune responses of dairy cows with Burkholderia contaminans-induced mastitis

Assessment of adaptive immune responses of dairy cows with Burkholderia contaminans-induced mastitis

Immunoproteomic analysis of the serum IgG response to cell wall-associated proteins of Staphylococcus aureus strains belonging to CC97 and CC151

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