Immunopurification of T-cells from sea bass Dicentrarchus labrax (L.)

Scapigliati, Giuseppe; Romano, Nicla; Abelli, Luigi; Meloni, Sabrina; Ficca, Anna Grazia; Buonocore, Francesco; Bird, Steve; Secombes, Chris J.

doi:10.1006/fsim.1999.0243

Cited by 62 publications

(47 citation statements)

References 33 publications

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“…Although some plankton-eating species such as the sea horse may have lost gut lymphoid populations during their evolution (18), these cells, named IELs, are probably present in the gut epithelium of most bony fish species. IELs prepared from the gut of sea bass expressed TCR␤ transcripts (19), and Ͼ90% of leukocytes isolated from the carp intestine were Ig-negative lymphoid cells (16). These observations suggest that fish IELs contain mainly T cells.…”

mentioning

confidence: 84%

Phenotypic and Functional Similarity of Gut Intraepithelial and Systemic T Cells in a Teleost Fish

Bernard

Six

Rigottier-Gois

et al. 2006

The Journal of Immunology

106

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Gut-associated lymphocytes were described in fish, but their involvement in immune responses is still unknown. In rainbow trout, intraepithelial lymphocytes (IELs) are scattered between gut epithelial cells, but neither Peyer’s patches nor mesenteric lymph nodes were identified. Rainbow trout IELs contain mainly T cells, because they expressed transcripts of T cell marker homologs of CD8, CD4, CD28, CD3ε, TCRζ, TCRγ, and TCRβ and lacked IgM. However, trout IELs did not show specific homing to the gut mucosa, which in mammals defines IELs as a distinctive mucosal population. A detailed analysis of the TCRβ repertoire of rainbow trout IELs was performed in both naive and virus-infected animals. TCRβ transcripts of rainbow trout IELs were highly diverse and polyclonal in adult naive individuals, in sharp contrast with the restricted diversity of IEL oligoclonal repertoires described in birds and mammals. Significant modifications of the trout IEL TCRβ repertoire were observed after a systemic infection with a fish rhabdovirus and were especially marked for Vβ4-bearing receptors as previously reported for spleen cells. Thus, we could not find any specific properties of the trout IEL TCRβ repertoire compared with the spleen and pronephros TCRβ repertoire, which questions the reality of a distinct IEL compartment in teleosts. Our findings suggest that a highly diversified αβ ΤCR repertoire is maintained in fish IELs in the absence of Peyer’s patches and mesenteric lymph nodes, whereas the restricted diversity of mouse αβ IELs is attributed to multiple cycles of activation and recirculation, allowing a progressive narrowing of the repertoire.

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mentioning

confidence: 84%

Phenotypic and Functional Similarity of Gut Intraepithelial and Systemic T Cells in a Teleost Fish

Bernard

Six

Rigottier-Gois

et al. 2006

The Journal of Immunology

106

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“…The TCR␤ locus of rainbow trout has been partly characterized, with a sequence description of the genomic region containing the D␤J␤C␤ genes (14). TCR sequences are now available for several other fish species, such as horned shark (Heterodontus francisci) (15), skate (Raja eglanteria) (16), catfish (17), and sea bass (18). In addition, the sequence and polymorphism of fish MHC strongly suggest that TCR can recognize the Ag in an MHC-restricted context (19 -21).…”

Section: Iral Hemorrhagic Septicemia Virus (Vhsv)mentioning

confidence: 99%

Rhabdovirus Infection Induces Public and Private T Cell Responses in Teleost Fish

Boudinot

Boubekeur

Benmansour

2001

The Journal of Immunology

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Many viruses induce a strong T cell response that contributes to the elimination of infected cells presenting viral peptides by MHC molecules. The structure and expression of genes encoding molecules homologous to mammalian αβ TCRs have been recently characterized in rainbow trout and in several teleost species, but the αβ T cell response against pathogens has not been directly demonstrated. To study the modifications of the T cell repertoire during an acute viral infection in rainbow trout, we adapted the immunoscope methodology, which consists of spectratyping the complementarity-determining region 3 length of the TCRβ chain. We showed that the naive T cell repertoire is polyclonal and highly diverse in the naive rainbow trout. Using viral hemorrhagic septicemia virus (VHSV), which provokes an acute infection in rainbow trout, we identified skewed complementarity-determining region 3 size profiles for several VβJβ combinations, corresponding to T cell clonal expansions during primary and secondary response to VHSV. Both public and private T cell expansions were shown by immunoscope analysis of spleen cells from several infected individuals of a rainbow trout clone sharing the same genetic background. The public response to VHSV consisted of expansion of Vβ4Jβ1 T cell, which appeared early during the primary response and was strongly boosted during the secondary response.

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“…Scattered lymphocytes have been observed both in the lamina propria (LP) or residing between epithelial cells. These last cells, designated as intraepithelial lymphocytes (IELs), have been observed in different species such as rainbow trout [5], carp [6] or sea bass [7]. All these studies suggested that the IELs were Ig-negative T cells.…”

Section: Introductionmentioning

confidence: 99%

The pyloric caeca area is a major site for IgM+ and IgT+ B cell recruitment in response to oral vaccination in rainbow trout

Ballesteros¹,

Saint-Jean²,

Pérez-Prieto³

et al. 2013

Fish & Shellfish Immunology

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Although previous studies have characterized some aspects of the immune response of the teleost gut in response to diverse pathogens or stimuli, most studies have focused on the posterior segments exclusively. However, there are still many details of how teleost intestinal immunity is regulated that remain unsolved, including the location of IgM + and IgT + B cells along the digestive tract and their role during the course of a local stimulus. Thus, in the current work, we have studied the B cell response in five different segments of the rainbow trout (Oncorhynchus mykiss) digestive tract in both naïve fish and fish orally vaccinated with an alginate-encapsulated DNA vaccine against infectious pancreatic necrosis virus (IPNV). IgM + and IgT + cells were identified all along the tract with the exception of the stomach in naïve fish. While IgM + cells were mostly located in the lamina propria (LP), IgT + cells were primarily localized as intraepithelial lymphocytes (IELs). Scattered IgM + IELs were only detected in the pyloric caeca. In response to oral vaccination, the pyloric caeca region was the area of the digestive tract in which a major recruitment of B cells was demonstrated through both real time PCR and immunohistochemistry, observing a significant increase in the number of both IgM + and IgT + IELs. Our findings demonstrate that both IgM + and IgT + respond to oral stimulation and challenge the paradigm that teleost IELs are exclusively T cells. Unexpectedly, we have also detected B cells in the fat tissue associated to the digestive tract that respond to vaccination, suggesting that these cells surrounded by adipocytes also play a role in mucosal defense.

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Immunopurification of T-cells from sea bass Dicentrarchus labrax (L.)

Cited by 62 publications

References 33 publications

Phenotypic and Functional Similarity of Gut Intraepithelial and Systemic T Cells in a Teleost Fish

Phenotypic and Functional Similarity of Gut Intraepithelial and Systemic T Cells in a Teleost Fish

Rhabdovirus Infection Induces Public and Private T Cell Responses in Teleost Fish

The pyloric caeca area is a major site for IgM+ and IgT+ B cell recruitment in response to oral vaccination in rainbow trout

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