Immunoreactive calcitonin production by human lung carcinoma cells in culture

Ellison, Morag; Woodhouse, D. L.; Hillyard, C. J.; Dowsett, Mitch; Coombes, R. Charles; Gilby, Edward D.; Greenberg, P. B.; Neville, A. Munro

doi:10.1038/bjc.1975.237

Cited by 96 publications

(32 citation statements)

References 14 publications

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“…In the second, a sample of medullarv thyroid carcinoma tissue was obtained at surgery from a patient known to have high levels of calcitonin. The adenylate cyclase response of membranes from this tumour was studied, and was found to be stimulated only bv gluca,gon (Table IV) The BEN cell line has been known for some time to produce CEA and calcitonin (Ellison et al, 1975). The latter has been measured by radioimmunoassay, and has been shown recently to be secreted in a form which is of higher molecular weight than monomeric human calcitonin, although mild trypsin treatment converts it to the size of CT-M on gel filtration (Coombes et al, 1977).…”

Section: Resultsmentioning

confidence: 99%

“…It is possible that the BEN cell population is not homogeneous, and contains one subpopulation which secretes calcitonin and one which has a calcitonin receptor. However, homogeneity, as assessed by morphological and functional (production of CEA and calcitonin) criteria, has been maintained over some 120 passages in 31 months (Ellison et al, 1975;Ellison, unpub. ) by increasing acetylcholinesterase production (Goldstein, Brodeur and Ross, 1973) and bind nerve growth factor at their membranes (Revoltella et al, 1974).…”

Section: Resultsmentioning

confidence: 99%

“…Earlier studies had indicated that the BEN calcitonin was biologically active in one in vitro system, since it inhibited 45Ca release from mouse calvaria maintained in culture (Ellison et al, 1975). Subsequent assays of partially purified media, however, have shown that the calcitonin from BEN cells is ineffective in lowering serum calcium in assay rats when given at doses (20 ng calcitonin immunoassay equivalents) which should be effective (Coombes et al, 1977 (Coombes et al, 1977), the biological activity seen in bone culture may be explained by trypsinlike activity in the bone culture system.…”

Section: Resultsmentioning

confidence: 99%

“…Cells were subcultured each week. They have continued to secrete CEA and calcitonin since the earlier report (Ellison et al, 1975 .…”

mentioning

confidence: 98%

“…A human cancer cell line which continuously secreted a large-mol.-wt form of human calcitonin (Ellison et al, 1975(Ellison et al, , 1976aCoombes et al, 1976Coombes et al, , 1977 (Ellison et al, 1975).…”

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confidence: 99%

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Calcitonin-responsive adenylate cyclase in a calcitonin-producing human cancer cell line

Hunt¹,

Ellison²,

Underwood³

et al. 1977

Br J Cancer

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Summary.-A calcitonin-responsive adenylate cyclase has been found in a cell line of a poorly differentiated bronchial carcinoma (BEN cells The characteristics of the adenylate cyclase response to SCT did not change over the period between cell adhesion (after subculture) and confluence. However, preincubation of cells for 4 h with SCT (150 nM) abolished the subsequent adenylate cyclase response of particulate preparations to further hormone. The practical difficulties encountered in purifying and quantifying the large-mol.-wt. form of CT-M secreted by BEN cells has precluded direct investigation of the potential relationship between hormone secretion and the occurrence of the calcitonin receptor. This relationship is discussed in terms of its possible biological significance.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…Cells were subcultured each week. They have continued to secrete CEA and calcitonin since the earlier report (Ellison et al, 1975 .…”

mentioning

confidence: 98%

mentioning

confidence: 99%

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Calcitonin-responsive adenylate cyclase in a calcitonin-producing human cancer cell line

Hunt¹,

Ellison²,

Underwood³

et al. 1977

Br J Cancer

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Calcium metabolism in cancer.Studies using calcium isotopes and immunoassays for parathyroid hormone and calcitonin

Coombes

Ward

Greenberg

et al. 1976

Cancer

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Studies of calcium metabolism in 38 patients with cancer indicated that: 1) intestinal absorption of calcium was reduced in patients with skeletal metastases and in those with hypercalcemia; 2) calcium-47 space (a measurement of bone turnover rate) was high in the patients with skeletal metastases; 3) hypercalcemic patients had higher urinary and endogenous fecal excretion of calcium than those who were normocalcemic; 4) levels of plasma immunoreactive parathyroid hormone were similar in normo-and hypercalcemic patients, but the levels for a given serum calcium in malignant disease were lower than those in primary hyperparathyroidism; a n d 5) some patients had elevated calcitonin levels. Hypercalcemia complicating malignant disease is therefore not due to hyperabsorption or diminished excretion of calcium, and a low calcium diet is unlikely to benefit these patients. Measurement of 47Ca space could be of use in monitoring therapy of patients with skeletal metastases, and measurement of plasma parathyroid hormone could be useful in the differential diagnosis of hypercalcemia.Cancer 38:2111-2120, 1976.KELETAL DESTRUCTION AND DISTURBED REGU-S lation of serum calcium are common colnplications of cancer. Most patients with hypercalcemia associated with cancer have skeletal m e t a~t a s e s ;~ it has been considered" that the elevation of serum calcium results, at least in part, from calcium release into the blood stream, arising from destruction of the bone by the inFrom

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Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung

Pettengill

Sorenson

Wurster‐Hill

et al. 1980

Cancer

160

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Sixteen continuous tumor-cell cultures have been isolated from 91 tissue specimens from patients with small-cell carcinoma of the lung. Biopsy and autopsy specimens of primary and metastatic tumors have been utilized. The developing cell lines were recognized by proliferation of tumor cells in the culture from one to 14 weeks after explanation and have been maintained for up to four years. Primary lung tumor, bone marrow aspirations, pleural effusions and other metastases have all been productive explant material for the development of cell lines. Their human origin has been demonstrated by chromosome and/or isoenzyme analysis. Dense core vesicles, characteristically found in small-cell tumor cells were observed by electron microscopic examination of cultured cells. Growth rates in vitro have been measured and the in vitro cycle time in tumors of one cell line (DMS 79) has been compared with in vivo cycle time in tumors arising from DMS 79 cells in nude athymic mice.

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Immunoreactive calcitonin production by human lung carcinoma cells in culture

Cited by 96 publications

References 14 publications

Calcitonin-responsive adenylate cyclase in a calcitonin-producing human cancer cell line

Calcitonin-responsive adenylate cyclase in a calcitonin-producing human cancer cell line

Calcium metabolism in cancer.Studies using calcium isotopes and immunoassays for parathyroid hormone and calcitonin

Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung

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