Immunoreactive GRP

Yanaihara, Noboru; Yanaihara, Chizuko; Mochizuki, Tohru; Iwahara, Kunihiro; Fujita, Tsuneo; Iwanaga, Toshihiko

doi:10.1016/0196-9781(81)90029-2

Cited by 139 publications

(25 citation statements)

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“…By using a recently developed specific radioimmunoassay for GRP [17], high concentrations have been detected in the mucosa and muscle of the body of the porcine stomach, in both layers of the antmm where the majority of gastrin cells are localized and in jejunal muscle [17]. The two molecular forms of GRP identified at these sites included one coeluting with synthetic porcine GRP and the other with porcine GRP- (14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27) [ 171. Immunohistochemical studies have shown numerous GRP immunoreactive nerve fibres in the myenteric nerve plexus of the porcine duodenum [17].…”

Section: Discussionmentioning

confidence: 99%

The Effect of the Mammalian Neuropeptide, Gastrin-Releasing Peptide (GRP), on Gastrointestinal and Pancreatic Hormone Secretion in Man

et al. 1983

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Gastrin-releasing peptide, a newly isolated mammalian peptide similar in its structure and actions to the amphibian peptide, bombesin, has recently been localized to nerves in the brain, gut and pancreas. The present study investigates its effects on gut and pancreatic peptides in man. Intravenous infusion of 0.7 and 2.9 pmol min-1 kg-1 produced significant elevation of plasma gastrin, cholecystokinin-like immunoreactivity and neurotensin. It was found also to potentiate glucose-dependent insulin secretion. Its specific location in nerve fibres in the proximal gut and pancreas and its selective effect on gastroenteropancreatic peptides may favour its role as a physiological regulatory neuropeptide.

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Section: Discussionmentioning

confidence: 99%

The Effect of the Mammalian Neuropeptide, Gastrin-Releasing Peptide (GRP), on Gastrointestinal and Pancreatic Hormone Secretion in Man

et al. 1983

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“…For example, several known neuropeptides, thyrotropin-releasing hormone (56), somatostatin, corticotropin-releasing factor (57), and prolactin (58), are also found in the pancreas. Even GRP (59,60) and other bombesin-like peptides (60,61) are apparently associated with nerve tissue. On the other hand, mammary gland epithelial cells or plasma cells recruited to the gland from the gut, which contain IgA (36), may secrete bombesin immunoreactivity.…”

Section: Resultsmentioning

confidence: 99%

A bombesin immunoreactive peptide in milk.

Jahnke¹,

Lazarus²

1984

Proc. Natl. Acad. Sci. U.S.A.

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Immunoreactivity to the amphibian peptide bombesin was found in instant nonfat dry milk (ca. 0.7 ng/ml) and in the whey of whole or skim bovine milk (ca. 1.2 ng/ml) even after ultracentrifugation. The soluble immunoreactivity was associated with a peptide exhibiting the following characteristics: (i) parallel displacement in an immunoassay using an antiserum recognizing bombesin amino acid residues 5-8; (ii) separation from both gastrin-releasing peptide and amphibian bombesin by gel filtration-the approximate Mr was 3,200; (iii) denaturation in urea, reduction by dithiothreitol, and acetylation by iodoacetamide had no effect on its elution profile by gel-filtration chromatography and the aggregation of added bombesin to milk proteins or peptides was not observed; (iv) reversed-phase HPLC separated milk immunoreactivity from gastrin-releasing peptide and bombesin; (v) digestion by trypsin yielded a smaller immunoreactive peptide fragment, whereas nearly all immunoreactivity was lost by treatment with a-chymotrypsin; and (vi) the level of immunoreactivity was unaffected by boiling. These data show that milk is an exogenous source of bombesin-like immunoreactivity,, which may account for the increase of gastric acid and gastrointestinal hormone levels after the consumption of milk.Immunoreactivity to bombesin, an amphibian tetradecapeptide (1), is distributed throughout mammalian tissues (2-5). Bombesin administered through intraperitoneal or intracisternal injection or intravenous infusion elicits diverse physiological effects in a variety of animal species, including humans (6-12). These effects include hypertension (1, 6, 13, 14), satiety (for review, see ref. 15), change in sugar metabolism (16, 17), hypothermia (18), modulation of the level of many gastrointestinal-associated peptide hormones (6)(7)(8)(9)(10)(11)(12)(19)(20)(21)(22)(23)(24), and increase of gastric acid secretion (7,10,13,14,19,20). Bombesin also releases both gastrin and gastric acid from isolated fundic mucosa (25) and acts as a spasmogen in isolated smooth muscle preparations (1,6,13,14,26 MATERIALS AND METHODSMilk. Pasteurized skim and whole milk from several different local dairies were purchased 5-7 days before the expiration date. Instant nonfat dry milk was the product of several major companies and reconstituted according to the manu- [Tyr8]Bombesin and GRP were iodinated every 4-6 weeks as described (43). The production of the antiserum to bombesin has been described (44). Optimum RIA conditions, which were systematically determined, are as follows: 0.1 M sodium cacodylate, pH 5.6/bovine serum albumin (5 mg/ml)/125I-labeled [Tyr8]bombesin (or GRP) (2,500 cpm)/antiserum (final dilution, 1:4,000)/standard or test solution in a total vol of 100 ,ul. Nonionic (Triton X-100, Nonidet P-40) and ionic (sodium lauryl sulfate) detergents above 0.1% and chaotropic agents (urea, (Fig. 1). Other known peptides, such as physalaemin, neurotensin, and braAbbreviations: RIA, radioimmunoassay; GRP, gastrin-releasing peptide (bombesin-lik...

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“…In addition, distention of the antrum also regulates the release of gastrin and somatostatin (Schubert and Makhlouf, 1992). GRP is found in porcine gastrointestinal tissues and pancreases (Yanaihara et al,1981) as well as in neurons of the colon and small intestine (Gonzalez et al, 2008), indicating that it is possibile that the release of hormones by G and D cells also may be regulated by neural regulation. Regulation of gastrin secretion occurs almost entirely through neural regulation and not via G cells alone.…”

Section: Discussionmentioning

confidence: 99%

<b>Expression patterns of T1R3 in duodenal epithelial cells with some gastrointestinal hormone</b>

Oda

Seta

Kataoka

et al. 2011

Archives of Histology and Cytology

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may regulate the secretion of serotonin by stimuli other than a mechanical one. IntroductionThe taste system, which is made up of taste receptor cells clustered in taste buds at the surface of the tongue and the soft palate, plays a key role in the decision to ingest or reject food. Its function is therefore, essential to protect organisms against harmful toxins and select the most appropriate nutrients. Ingested nutrients stimulate the secretion of gastrointestinal hormones necessary for the coordinated processes of food digestion.The T1Rs, G-protein-coupled receptors (GPCRs) that form two novel seven-transmembrane domains, are expressed in topographically distinct subpopulations of taste receptor cells and taste buds (Hoon et al., 1999). The functional expression of T1Rs is influenced by sweet and umami taste stimuli. A T1R2/T1R3 heterodimer serves as a chemical receptor for sweet tastes (diverse natural and synthetic sweeteners). T1R1/T1R3 heterodimers are receptors for the umami compound l-glutamate, a response enhanced by 5'-ribonucleotides (Nelson et al., 2001;Li et al., 2002).Recent studies have demonstrated that solitary cells expressing taste cell signal transduction proteins exist in hollow organs. It has been previously shown that T1Rs are expressed in the gastrointestinal tract (GI) (Hofer et al., 1996;Wu et al., 2002; Dyer et al., 2005;Grundy, 2005). They are additionally expressed in the stomach, small intestine -including the duodenum, and colon in mice and humans, with the exception of T1R2, which has not been detected in the mouse or human stomach or in the mouse colon (Bezençon et al., 2007

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Immunoreactive GRP

Cited by 139 publications

References 9 publications

The Effect of the Mammalian Neuropeptide, Gastrin-Releasing Peptide (GRP), on Gastrointestinal and Pancreatic Hormone Secretion in Man

The Effect of the Mammalian Neuropeptide, Gastrin-Releasing Peptide (GRP), on Gastrointestinal and Pancreatic Hormone Secretion in Man

A bombesin immunoreactive peptide in milk.

<b>Expression patterns of T1R3 in duodenal epithelial cells with some gastrointestinal hormone</b>

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