Immunoreactive relaxin in seminal plasma of fertile boars and its correlation with sperm motility characteristics determined by computer‐assisted digital image analysis

Sasaki, Yoshio; Kohsaka, Tetsuya; Kawarasaki, Tatsuo; Sasada, Hiroshi; Ogine, T.; Bamba, Kimio; Takahara, Hidenari

doi:10.1046/j.1365-2605.2001.00259.x

Cited by 30 publications

(34 citation statements)

References 32 publications

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“…Testosterone, E 2 , LH, and FSH assay Serum concentrations of testosterone and E 2 were measured with a TR-FIA kit (Perkin-Elmer), as previously reported (Sasaki et al 2001). Testosterone and E 2 were extracted from the serum samples with diethyl ether before being applied to the kits.…”

Section: Development Of Tr-fia For Boar Insl3mentioning

confidence: 99%

Dynamics of insulin-like factor 3 and its receptor expression in boar testes

Minagawa¹,

Sagata²,

Pitia³

et al. 2014

Journal of Endocrinology

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Relaxin-like factor (RLF), now mainly known as insulin-like factor 3 (INSL3), is essential for testis descent during fetal development; however, its function in the adult testis is still being elucidated. As a major step toward understanding the as-yet-unknown function of INSL3 in boars, this study aimed to develop a time-resolved fluoroimmunoassay for boar INSL3, characterize the dynamics of INSL3 expression during development, and demonstrate the expression of the INSL3 hormone-receptor system in the testis. All samples were collected from Duroc boars. The sensitivity of the assay system established was 8.2 pg/well (164 pg/ml), and no cross-reactivity with other hormones, such as porcine relaxin, was observed. Circulating INSL3 was shown to increase progressively during development. INSL3 secreted from the Leydig cells was released not only into the blood circulation but also into the interstitial and seminiferous compartments in sufficient concentrations. A testicular fractionation study revealed that its receptor RXFP2 transcripts were expressed mainly in testicular germ cells. In addition, INSL3 bound to the germ cell membranes in a hormone-specific and saturable manner. These results reveal that INSL3 secreted into the interstitial compartment from the Leydig cells is transported into the seminiferous compartments, where its receptor RXFP2 is expressed mainly in the germ cells to which INSL3 binds, suggesting that INSL3 functions as a paracrine factor on seminiferous germ cells.

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Section: Development Of Tr-fia For Boar Insl3mentioning

confidence: 99%

Dynamics of insulin-like factor 3 and its receptor expression in boar testes

Minagawa¹,

Sagata²,

Pitia³

et al. 2014

Journal of Endocrinology

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“…Initially, it was thought that the relaxin produced in the male reproductive tract would be released exclusively in the seminal fluid to influence sperm motility (Sasaki et al 2001, Kohsaka et al 2003, Sherwood 2004. However, studies with relaxin gene knockout animals (M1RKO) show that relaxin also plays a role in growth and development of the male reproductive system (Samuel et al 2003a).…”

Section: Introductionmentioning

confidence: 99%

Locally produced relaxin may affect testis and vas deferens function in rats

Cardoso¹,

Nascimento²,

Royer³

et al. 2010

Reproduction

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We have previously shown that the rat testis and vas deferens contain high levels of the relaxin receptor, RXFP1. The present study was undertaken to determine the expression of relaxin in these tissues, and the effect of exogenous relaxin on Sertoli cell proliferation and on the mRNA levels of some proteins that may contribute to epithelial secretion and tissue reorganization in the vas deferens. Relaxin mRNA levels in testis and vas deferens were much lower than in the prostate. Sertoli cells seem to be an important source of relaxin mRNA in testis. Relaxin immunoreactivity was detected in the seminiferous epithelium but not in the interstitial compartment. The relaxin precursor was expressed in the vas deferens, and relaxin immunoreactivity was detected in apical cells of the vas deferens. Castration, but not treatment with the anti-estrogen ICI 182,780, dramatically reduced relaxin mRNA levels in the prostate and vas deferens, and this effect was prevented by testosterone. Rxfp1 mRNA levels in the vas deferens and prostate were not affected by castration or treatment with ICI 182,780. Exogenous relaxin increased the incorporation of 3 H-thymidine in cultured Sertoli cells, and treatment of the vas deferens with 100 ng/ml relaxin increased the mRNA levels for the cystic fibrosis chloride channel (cystic fibrosis transmembrane regulator) about three times, and doubled mRNA levels for the inducible form of nitric oxide synthase and metalloproteinase 7. These results suggest that locally produced relaxin acts as an autocrine or paracrine agent in the testis and vas deferens to affect spermatogenesis and seminal fluid composition.

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“…Relaxin has been identified in roosters [2], boar testes [3], and in human seminal plasma [4]. The accessory glands are the source of seminal relaxin, which is secreted into the seminal plasma [5] and saturated with sperm just after ejaculation [6]. Relaxin has physiological influences on sperm motility in some species of mammal [7].…”

mentioning

confidence: 99%

Effect of Relaxin on Acrosome Reaction and Utilization of Glucose in Boar Spermatozoa

Miah

Tareq

Hamano

et al. 2006

Journal of Reproduction and Development

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Abstract.Relaxin is a peptide hormone found in seminal plasma that has a physiological influence on sperm motility in some species. There are no reports on the effect of relaxin on acrosome reaction and utilization of glucose in boar spermatozoa. In this study, to investigate the effects of relaxin on sperm motility, acrosome reaction, and incorporation and oxidation of labeled glucose, boar spermatozoa were washed and preincubated for swim-up and then incubated (0-6 h) with 0, 20, or 40 ng/ml relaxin in mTALP medium. The results indicated that the addition of relaxin stimulated sperm motility significantly (P<0.05) during 1-4 h of incubation. The percentage of acrosome-reacted live spermatozoa was higher (P<0.05) when the spermatozoa were treated with 20 or 40 ng/ml relaxin. The rate of incorporation, and oxidation of glucose were also greater (P<0.05) in the spermatozoa incubated with relaxin compared to the control spermatozoa. The rate of incorporation and oxidation of 14 C-glucose were increased in correlation with acrosome reaction up to 4 h of incubation and then decreased in line with the increasing incubation period. In conclusion, the present study demonstrates that relaxin accelerates not only motility but also the acrosome reaction and utilization of glucose in boar spermatozoa.

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Immunoreactive relaxin in seminal plasma of fertile boars and its correlation with sperm motility characteristics determined by computer‐assisted digital image analysis

Cited by 30 publications

References 32 publications

Dynamics of insulin-like factor 3 and its receptor expression in boar testes

Dynamics of insulin-like factor 3 and its receptor expression in boar testes

Locally produced relaxin may affect testis and vas deferens function in rats

Effect of Relaxin on Acrosome Reaction and Utilization of Glucose in Boar Spermatozoa

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