Immunoreactive relaxin surge in the peritoneal fluid of women during the midluteal phase

OBJECTIVE To determine if preimplantation embryos are targets for relaxin secreted from the corpus luteum of the menstrual cycle. DESIGN Rhesus monkey oocytes obtained from females undergoing controlled ovarian hyperstimulation were inseminated and the resulting embryos were cultured in medium with or without recombinant human relaxin (20 ng/ml) for 8 days. SETTING Research laboratory. ANIMALS Rhesus monkey. INTERVENTIONS Controlled ovarian stimulation to obtain oocytes for in vitro produced embryos that were cultured with or without human recombinant relaxin. MAIN OUTCOME MEASURES The rate of blastocyst development and the percentage of blastocysts and ICM/TE ratio were measured on Day 8 of culture. The presence of relaxin receptor (RXFP1) mRNA in 8 cell embryos was observed by array hybridization. RESULTS RXFP1 receptor expression was localized to the inner cell mass of blastocysts as shown by immunohistochemistry. The percentage of embryos that developed to blastocyst and the inner cell mass/ trophectoderm cell ratio was unchanged with relaxin supplementation, however the relaxin-treated embryos developed into blastocysts significantly sooner than untreated embryos. CONCLUSIONS These results are the first evidence that the preimplantation primate embryo is a target for relaxin and that the addition of relaxin to in vitro culture medium enhances rhesus monkey embryo development.

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Primate preimplantation embryo is a target for relaxin during early pregnancy

VandeVoort

Mtango

Latham

et al. 2011

Fertility and Sterility

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Intranasal gonadotropin-releasing hormone agonist (GnRHa) for luteal-phase support following GnRHa triggering, a novel approach to avoid ovarian hyperstimulation syndrome in high responders

Bar‐Hava

Mizrachi

Karfunkel-Doron³

et al. 2016

Fertility and Sterility

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Gonadotropin-releasing hormone analogue as sole luteal support in antagonist-based assisted reproductive technology cycles

Hava

Blueshtein

Herman

et al. 2017

Fertility and Sterility

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Immunoreactive relaxin surge in the peritoneal fluid of women during the midluteal phase

Cited by 15 publications

References 20 publications

Primate preimplantation embryo is a target for relaxin during early pregnancy

Primate preimplantation embryo is a target for relaxin during early pregnancy

Intranasal gonadotropin-releasing hormone agonist (GnRHa) for luteal-phase support following GnRHa triggering, a novel approach to avoid ovarian hyperstimulation syndrome in high responders

Gonadotropin-releasing hormone analogue as sole luteal support in antagonist-based assisted reproductive technology cycles

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