Immunoreactive α2a-adrenoceptors and associated regulatory proteins in brains of suicides

García-Sevilla, J.A.; Escribá, Pablo V.; Harpe, R. La; Harding, T.; Walzer, Claude; Guimón, José

doi:10.1016/s0006-3223(97)88093-6

Biological Psychiatry

1997

DOI: 10.1016/s0006-3223(97)88093-6

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Immunoreactive α2a-adrenoceptors and associated regulatory proteins in brains of suicides

J.A. García-Sevilla¹,

Pablo V. Escribá²,

R. La Harpe³

et al.

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1999

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“…The present study was designed to evaluate in parallel the levels of immunolabeled α 2A ‐adrenoceptors and associated regulatory proteins (Gα i1/2 and GRK2/3) in brains of suicide and depressed suicide victims. A preliminary account of part of this study was given at the Sixth World Congress of Biological Psychiatry (García‐Sevilla et al, 1997 a ).…”

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confidence: 99%

Up‐Regulation of Immunolabeled α_2A‐Adrenoceptors,G_i Coupling Proteins, and Regulatory Receptor Kinases in the Prefrontal Cortex of Depressed Suicides

Garcı́a-Sevilla

Escribá

Ozaita

et al. 1999

Journal of Neurochemistry

147

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Suicide and depression are associated with an increased density of ␣ 2 -adrenoceptors (radioligand receptor binding) in specific regions of the human brain. The function of these inhibitory receptors involves various regulatory proteins (G i coupling proteins and G proteincoupled receptor kinases, GRKs), which work in concert with the receptors. In this study we quantitated in parallel the levels of immunolabeled ␣ 2A -adrenoceptors and associated regulatory proteins in brains of suicide and depressed suicide victims. Specimens of the prefrontal cortex (Brodmann area 9) were collected from 51 suicide victims and 31 control subjects. Levels of ␣ 2A -adrenoceptors, G␣ i1/2 proteins, and GRK 2/3 were assessed by immunoblotting techniques by using specific polyclonal antisera and the immunoreactive proteins were quantitated by densitometry. Increased levels of ␣ 2A -adrenoceptors (31-40%), G␣ i1/2 proteins (42-63%), and membrane-associated GRK 2/3 (24 -32%) were found in the prefrontal cortex of suicide victims and antidepressantfree depressed suicide victims. There were significant correlations between the levels of GRK 2/3 (dependent variable) and those of ␣ 2A -adrenoceptors and G␣ i1/2 proteins (independent variables) in the same brain samples of suicide victims (r ϭ 0.56, p ϭ 0.008) and depressed suicide victims (r ϭ 0.54, p ϭ 0.041). Antemortem antidepressant treatment was associated with a significant reduction in the levels of G␣ i1/2 proteins (32%), but with modest decreases in the levels of ␣ 2A -adrenoceptors (6%) and GRK 2/3 (18%) in brains of depressed suicide victims. The increased levels in concert of ␣ 2A -adrenoceptors, G␣ i1/2 proteins, and GRK 2/3 in brains of depressed suicide victims support the existence of supersensitive ␣ 2A -adrenoceptors in subjects with major depression. Key Words: ␣ 2A -Adrenoceptors-G proteins-G protein-coupled receptor kinase-Human brain-Suicide-Depression.

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mentioning

confidence: 99%

Up‐Regulation of Immunolabeled α_2A‐Adrenoceptors,G_i Coupling Proteins, and Regulatory Receptor Kinases in the Prefrontal Cortex of Depressed Suicides

Garcı́a-Sevilla

Escribá

Ozaita

et al. 1999

Journal of Neurochemistry

147

View full text Add to dashboard Cite

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Immunoreactive α2a-adrenoceptors and associated regulatory proteins in brains of suicides

Cited by 1 publication

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Up‐Regulation of Immunolabeled α_2A‐Adrenoceptors,G_i Coupling Proteins, and Regulatory Receptor Kinases in the Prefrontal Cortex of Depressed Suicides

Up‐Regulation of Immunolabeled α_2A‐Adrenoceptors,G_i Coupling Proteins, and Regulatory Receptor Kinases in the Prefrontal Cortex of Depressed Suicides

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Immunoreactive α2a-adrenoceptors and associated regulatory proteins in brains of suicides

Cited by 1 publication

References 0 publications

Up‐Regulation of Immunolabeled α2A‐Adrenoceptors,Gi Coupling Proteins, and Regulatory Receptor Kinases in the Prefrontal Cortex of Depressed Suicides

Up‐Regulation of Immunolabeled α2A‐Adrenoceptors,Gi Coupling Proteins, and Regulatory Receptor Kinases in the Prefrontal Cortex of Depressed Suicides

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Up‐Regulation of Immunolabeled α_2A‐Adrenoceptors,G_i Coupling Proteins, and Regulatory Receptor Kinases in the Prefrontal Cortex of Depressed Suicides

Up‐Regulation of Immunolabeled α_2A‐Adrenoceptors,G_i Coupling Proteins, and Regulatory Receptor Kinases in the Prefrontal Cortex of Depressed Suicides