Immunoreactivity and bioactivity of lipopolysaccharide-binding protein in normal and heat-inactivated sera

Mészáros, Károly; Aberle, S. B. D.; White, Mark L.; Parent, J B

doi:10.1128/iai.63.1.363-365.1995

Cited by 36 publications

(15 citation statements)

References 27 publications

(21 reference statements)

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“…Serum levels of LBP were quantified by enzymelinked immunosorbent assay as previously described. 31,32 Briefly, solutions containing LBP were incubated in microtiter wells coated with affinity-purified rabbit anti-human recombinant LBP (rLBP). After washing, biotin-labeled anti-rLBP was added, followed by alkaline phosphatase-labeled streptavidin and the chromogenic substrate p-nitrophenylphosphate.…”

Section: Specialized Laboratory Assaysmentioning

confidence: 99%

Preoperative and Intraoperative Predictors of Postoperative Morbidity, Poor Graft Function, and Early Rejection in 190 Patients Undergoing Liver Transplantation

et al. 2001

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Subjects: A total of 190 adult patients undergoing primary liver transplantation. Main Outcome Measure: Adverse outcome was prospectively defined as either in-hospital death or prolonged postoperative hospitalization (Ͼ14 days) associated with morbidity. Potential preoperative and intraoperative risk factors were collected. Associations were tested by univariate analysis followed by multivariate analysis in which preoperative factors were entered before intraoperative factors. Results: Adverse outcome occurred in 44.7% of patients. Incidences of other complications were as follows: inhospital mortality (8.4%), primary graft nonfunction (4.2%), poor early graft function (1.1%), and early rejection (31.2%). Univariate predictors of adverse outcome were United Network for Organ Sharing status (P=.003), Child-Turcotte-Pugh score (P=.02), POSSUM physiological score (P=.002), recipient age (P=.01), preoperative serum high-density lipoprotein cholesterol level (P=.03), preoperative serum creatinine level (P=.002), preoperative serum total IgG level (P=.004), duration in hospital preoperatively (P=.03), operative duration (PϽ.001), allogeneic erythrocyte transfusions (PϽ.001), total intraoperative fluids (P=.002), and use of inotropic agents (P=.01). In the final multivariate model, predictors of adverse outcome were United Network for Organ Sharing status (P = .03), recipient age (P=.002), and total intraoperative fluids (P=.04). Most patients who died or had a prolonged hospitalization exhibited dysfunction of more than 1 organ system, including pulmonary, renal, and infectious complications. Conclusions: Adverse outcome occurs frequently after liver transplantation, usually involves multiple organ systems, and is predicted in part by several preoperative and intraoperative factors.

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Section: Specialized Laboratory Assaysmentioning

confidence: 99%

Preoperative and Intraoperative Predictors of Postoperative Morbidity, Poor Graft Function, and Early Rejection in 190 Patients Undergoing Liver Transplantation

et al. 2001

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“…One factor that is at least partially retained in heat‐inactivated FBS is LBP (Meszaros et al ., ). Lipopolysaccharide‐binding protein binds to either free LPS or to bacterial surfaces and catalyses an interaction with CD14 and TLR4 (Wright et al ., 1989; 1990; Kitchens, ; da Silva Correia et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Yersinia pestistargets neutrophils via complement receptor 3

et al. 2014

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Yersinia species display a tropism for lymphoid tissues during infection, and the bacteria select innate immune cells for delivery of cytotoxic effectors by the type III secretion system. Yet the mechanism for target cell selection remains a mystery. Here we investigate the interaction of Yersinia pestis with murine splenocytes to identify factors that participate in the targeting process. We find that interactions with primary immune cells rely on multiple factors. First, the bacterial adhesin Ail is required for efficient targeting of neutrophils in vivo. However, Ail does not appear to directly mediate binding to a specific cell type. Instead, we find that host serum factors direct Y. pestis to specific innate immune cells, particularly neutrophils. Importantly, specificity towards neutrophils was increased in the absence of bacterial adhesins due to reduced targeting of other cell types, but this phenotype was only visible in the presence of mouse serum. Addition of antibodies against complement receptor 3 and CD14 blocked target cell selection, suggesting that a combination of host factors participate in steering bacteria toward neutrophils during plague infection.

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“…LBP binds to LOS or LPS and transports LOS or LPS to either sCD14 in serum or CD14 on the cell surface, which interacts with another unknown receptor responsible for initiating signal transduction events, leading to TNF-␣ expression (60). Most human epithelial cells do not express CD14 but can still be stimulated with LOS or LPS to express TNF-␣ if the culture medium contains serum that has sCD14 and LBP (40), both of which can be supplied by FBS (used in our studies) but are not as active as their human counterparts (27). One reason for the absence of a TNF-␣ response in our coculture system may be that the HS27 or HaCaT cells do not express CD14 and/or that the serum does not contain active sCD14 or LPB.…”

Section: Discussionmentioning

confidence: 99%

Examination of Early Interactions between Haemophilus ducreyi and Host Cells by Using Cocultured HaCaT Keratinocytes and Foreskin Fibroblasts

Zaretzky

Kawula

1999

Infect Immun

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Haemophilus ducreyi is the etiologic agent of chancroid, a sexually transmitted genital ulcer disease. Keratinocytes are likely the first cell type encountered by H. ducreyiupon infection of human skin; thus, the interaction between H. ducreyi and keratinocytes is probably important for the ability of H. ducreyi to establish infection. We have used the HaCaT keratinocyte cell line grown in monolayers and in cocultures with HS27 fibroblasts to investigate H. ducreyi interactions with keratinocytes and the host-cell response to H. ducreyiinfection. Using quantitative adherence and gentamicin protection assays, we determined that approximately 13% of H. ducreyiadhered to HaCaT cell monolayers, while only a small proportion (0.0052%) was intracellular. By transmission electron microscopy, we observed numerous H. ducreyi organisms adherent to but rarely within HaCaT cells cocultured with fibroblasts. Both liveH. ducreyi and purified H. ducreyilipooligosaccharide (LOS) induced significant interleukin 8 (IL-8) expression from HaCaT cell-HS27 cell cocultures. However, the level of IL-8 expression in response to LOS alone was not as pronounced.H. ducreyi LOS was a more potent inducer of IL-8 from cocultures than Escherichia coli lipopolysaccharide (LPS) at the same concentration, suggesting a unique effect of H. ducreyi LOS on cocultures. Neither live H. ducreyinor purified H. ducreyi LOS or E. coli LPS induced tumor necrosis factor alpha expression from cocultures.H. ducreyi induced drastically different cytokine profiles from cocultures than from HS27 or HaCaT cells cultured separately. IL-8 expression by skin cells in response to H. ducreyiinfection in vivo may be responsible for the massive influx of polymorphonuclear leukocytes and other inflammatory cells to the site of infection. This influx of inflammatory cells may be partly responsible for the tissue destruction characteristic of chancroid.

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Immunoreactivity and bioactivity of lipopolysaccharide-binding protein in normal and heat-inactivated sera

Cited by 36 publications

References 27 publications

Preoperative and Intraoperative Predictors of Postoperative Morbidity, Poor Graft Function, and Early Rejection in 190 Patients Undergoing Liver Transplantation

Preoperative and Intraoperative Predictors of Postoperative Morbidity, Poor Graft Function, and Early Rejection in 190 Patients Undergoing Liver Transplantation

Yersinia pestistargets neutrophils via complement receptor 3

Examination of Early Interactions between Haemophilus ducreyi and Host Cells by Using Cocultured HaCaT Keratinocytes and Foreskin Fibroblasts

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