Immunoreactivity of the calbindin D28k in the parahippocampal gyrus of chinchilla

Abstract

Ten adult male chinchillas were used. The localisation of calbindin D28k (CB) was examined with the use of two types of reactions: immunocytochemical peroxidase-antiperoxidase and immunofluorescence staining with a specific monoclonal antibody against CB. Immunocytochemical examination demonstrated the presence of CB-positive neurons in the following layers of all parts the parahippocampal gyrus (PG): marginal, external cellular, middle cellular, and internal cellular, i.e. in entorhinal area, parasubiculum, and presubiculum. Immunofluorescence staining revealed the presence of CB in both Hu C/Dimmunoreactive (IR) neurons and nervous fibers of the PG. CB-IR neuronal cell bodies were moderately numerous (ca. 10% of Hu C/D-IR neurons) and clearly distinguished from the background. Each layer of the brain area consisted of two types of neurons: pyramidal and multiform. Among the second type of neurons, four kinds of morphologically different neuronal subclasses were observed: multipolar, bipolar, round, and Cajal-Retzius cells. It is concluded that the expression of CB in the PG of the chinchilla is species specific and limited to several subclasses of neurons.

Immunocytochemical detection of calretinin in the claustrum and endopiriform nucleus of the chinchilla

“…Although the physiological role(s) of CR are far from being fully understood, it is generally accepted that it acts as a neuronal modulator and regulator of synaptic plasticity Kuźnicki 1999, Schwaller et al 2002). Because of its specific localization in CNS, CR has received growing research attention in recent years (Maskey et al 2012, Szalak et al 2013, Bae et al 2015. Using the immunohistochemical technique the distribution patterns of CR have been described in discrete regions of the mammalian brain including the dolphin (Cozzi et al 2014), rodents (Edelstein et al 2010, Druga et al 2015, rabbit , cat (Rahman and Baizer 2007), monkey (Reynhout and Baizer 1999) and human (Prensa et al 2003).…”