Immunoregulatory CD71+ Erythroid Cells (CECs) Expand in Multiple Myeloma and Impair Control of <i>L. Monocytogenes</i> Infection

Grzywa, Tomasz M.; Czubak, Karol; Sidor, Karolina; Pilch, Zofia; Bragiel-Pieczonka, Aneta M; Hoser, Grażyna; Rodziewicz-Lurzynska, Anna; Małecka‐Giełdowska, Milena; Barankiewicz, Joanna; Garbicz, Filip; Ciepiela, Olga; Juszczyński, Przemysław; Węgrzynowicz, Michał; Skirecki, Tomasz; Gołąb, Jakub; Nowis, Dominika

doi:10.1182/blood-2022-164577

Cited by 1 publication

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“…Extensive investigations involving both human and murine erythroid cells, facilitated by techniques such as flow cytometry, bulk RNA-seq, and qPCR, have elucidated their capacity to generate and express a diverse array of immunosuppressive molecules. Noteworthy among these are interleukin-10 (IL-10) [ 2 , 3 ] and transforming growth factor-beta 1 (TGF-beta1) [ 4 , 5 ] cytokines, as well as the production of reactive oxygen species (ROS) [ 6 , 7 ]. Additionally, erythroid cells have been found to express programmed cell death ligand-1 (PD-L1) [ 5 ], PD-L2 [ 6 ], and V-domain Ig suppressor of T cell activation (VISTA) [ 8 ] inhibitory checkpoint molecules, along with the enzymatic activities of Arginase-1 and Arginase-2 [ 5 , 9 – 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…Noteworthy among these are interleukin-10 (IL-10) [ 2 , 3 ] and transforming growth factor-beta 1 (TGF-beta1) [ 4 , 5 ] cytokines, as well as the production of reactive oxygen species (ROS) [ 6 , 7 ]. Additionally, erythroid cells have been found to express programmed cell death ligand-1 (PD-L1) [ 5 ], PD-L2 [ 6 ], and V-domain Ig suppressor of T cell activation (VISTA) [ 8 ] inhibitory checkpoint molecules, along with the enzymatic activities of Arginase-1 and Arginase-2 [ 5 , 9 – 11 ].…”

Section: Introductionmentioning

confidence: 99%

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A subpopulation of human bone marrow erythroid cells displays a myeloid gene expression signature similar to that of classic monocytes

Perik-Zavodskii,

Perik-Zavodskaia,

Shevchenko

et al. 2024

PLoS ONE

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Erythroid cells, serving as progenitors and precursors to erythrocytes responsible for oxygen transport, were shown to exhibit an immunosuppressive and immunoregulatory phenotype. Previous investigations from our research group have revealed an antimicrobial gene expression profile within murine bone marrow erythroid cells which suggested a role for erythroid cells in innate immunity. In the present study, we focused on elucidating the characteristics of human bone marrow erythroid cells through comprehensive analyses, including NanoString gene expression profiling utilizing the Immune Response V2 panel, a BioPlex examination of chemokine and TGF-beta family proteins secretion, and analysis of publicly available single-cell RNA-seq data. Our findings demonstrate that an erythroid cell subpopulation manifests a myeloid-like gene expression signature comprised of antibacterial immunity and neutrophil chemotaxis genes which suggests an involvement of human erythroid cells in the innate immunity. Furthermore, we found that human erythroid cells secreted CCL22, CCL24, CXCL5, CXCL8, and MIF chemokines. The ability of human erythroid cells to express these chemokines might facilitate the restriction of immune cells in the bone marrow under normal conditions or contribute to the ability of erythroid cells to induce local immunosuppression by recruiting immune cells in their immediate vicinity in case of extramedullary hematopoiesis.

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