Immunoregulatory properties of mast cell-derived exosomes

Dimitris, Skokos; Goubran‐Botros, Hany; Roa, Michèle; Mécheri, Salaheddine

doi:10.1016/s0161-5890(02)00088-3

Cited by 45 publications

(15 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These vesicles contain various immunoregulatory proteins as well as mRNAs and microRNAs [27,57,58]. Evidence has shown that mast cell-derived exosomes are able to induce lymphocyte proliferation and cytokine production [30,59], and can also induce mediator release from endothelial cells [28].…”

Section: Discussionmentioning

confidence: 99%

Secreted Factors from Human Mast Cells Trigger Inflammatory Cytokine Production by Human Airway Smooth Muscle Cells

Xia

Harris²,

Stewart³

et al. 2012

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Background: A notable feature of allergic asthma is the infiltration of mast cells into smooth muscle in the human airway. Thus, mast cells and human airway smooth muscle (hASM) cells are likely to exhibit mutual functional modulation via direct cell-cell contact or through released factors. This study examined mast cell modulation of hASM cell cytokine release. Methods: The mast cell line HMCα was used to model mast cell function. hASM cells were either co-cultured directly with resting or IgE/antigen-stimulated HMCα cells or treated with HMCα-conditioned media to examine the impact on cytokine release. The activation pathways triggered in hASM cells by the mast cell-derived factors were examined through the use of selective inhibitors and by Western blotting. Results: HMCα cells, or their conditioned media, induced the expression of cytokines (IL-8 and IL-6) by hASM cells at both the mRNA and the protein level. Cytokine expression in hASM cells was greatly amplified when HMCα cells were IgE/antigen-activated. The effects of the conditioned media were not mediated by the chemokines MCP-1 and MIP-1α or by exosomes. While the mast cell-derived factor(s) increased p38^MAPK phosphorylation in hASM cells, cytokine production was not inhibited by the p38^MAPK inhibitor SB203580. hASM cell production of IL-8 induced by HMCα condition media but not IL-6 was, however, attenuated by the Src tyrosine kinase inhibitor PP2. Conclusions: Our study shows that the release of soluble mediators by activated mast cells can stimulate hASM cells to elicit production of proinflammatory cytokines that may then exacerbate airway inflammation in asthma.

show abstract

Section: Discussionmentioning

confidence: 99%

Secreted Factors from Human Mast Cells Trigger Inflammatory Cytokine Production by Human Airway Smooth Muscle Cells

Xia

Harris²,

Stewart³

et al. 2012

Int Arch Allergy Immunol

View full text Add to dashboard Cite

show abstract

“…Furthermore, it has been demonstrated that in cultured cortical neurons, the exosomal release is modulated by glutaminergic activity [46]. Cell activation by crosslinking of receptors, such as IgE in mast cells [47,48] or CD3 in T-cells, triggers the release of exosomes [49]. In macrophages and DCs, the P2X purine receptor 7 regulates the ATP-mediated release of exosomes [50].…”

Section: Regulation Of Exosome Secretionmentioning

confidence: 99%

Signaling Pathways in Exosomes Biogenesis, Secretion and Fate

Urbanelli

Magini

Buratta

et al. 2013

Genes

315

281

View full text Add to dashboard Cite

Exosomes are small extracellular vesicles (30–100 nm) derived from the endosomal system, which have raised considerable interest in the last decade. Several studies have shown that they mediate cell-to-cell communication in a variety of biological processes. Thus, in addition to cell-to-cell direct interaction or secretion of active molecules, they are now considered another class of signal mediators. Exosomes can be secreted by several cell types and retrieved in many body fluids, such as blood, urine, saliva and cerebrospinal fluid. In addition to proteins and lipids, they also contain nucleic acids, namely mRNA and miRNA. These features have prompted extensive research to exploit them as a source of biomarkers for several pathologies, such as cancer and neurodegenerative disorders. In this context, exosomes also appear attractive as gene delivery vehicles. Furthermore, exosome immunomodulatory and regenerative properties are also encouraging their application for further therapeutic purposes. Nevertheless, several issues remain to be addressed: exosome biogenesis and secretion mechanisms have not been clearly understood, and physiological functions, as well as pathological roles, are far from being satisfactorily elucidated.

show abstract

“…6 Exosomes are secreted by a multitude of other cells including those of hematopoietic lineage, such as cytotoxic T cells, 8 antigen-presenting B cells, 9 and dendritic cells, 10 where they play an immunoregulatory role. 11 Proteomic analysis of exosomes secreted from various cell types has revealed the presence of ubiquitous proteins such as tubulin, actin, and actin-binding proteins, as well as cell type-specific proteins. 12,13 Secreted platelet proteins act in an autocrine or paracrine fashion to modulate cell signaling.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the proteins released from activated platelets leads to localization of novel platelet proteins in human atherosclerotic lesions

Coppinger

Cagney

Toomey

et al. 2004

Blood

726

540

View full text Add to dashboard Cite

Proteins secreted by activated platelets can adhere to the vessel wall and promote the development of atherosclerosis and thrombosis. Despite this biologic significance, however, the complement of proteins comprising the platelet releasate is largely unknown. Using a proteomics approach, we have identified more than 300 proteins released by human platelets following thrombin activation. Many of the proteins identified were not previously attributed to platelets, including secretogranin III, a potential monocyte chemoattractant precursor; cyclophilin A, a vascular smooth muscle cell growth factor; calumenin, an inhibitor of the vitamin K epoxide reductase-warfarin interaction, as well as proteins of unknown function that map to expressed sequence tags. Secretogranin III, cyclophilin A, and calumenin were confirmed to localize in platelets and to be released upon activation. Furthermore, while absent in normal vasculature, they were identified in human atherosclerotic lesions. Therefore, these and other proteins released from platelets may contribute to atherosclerosis and to the thrombosis that complicates the disease. Moreover, as soluble extracellular proteins, they may prove suitable as novel therapeutic targets.

show abstract

Immunoregulatory properties of mast cell-derived exosomes

Cited by 45 publications

References 26 publications

Secreted Factors from Human Mast Cells Trigger Inflammatory Cytokine Production by Human Airway Smooth Muscle Cells

Secreted Factors from Human Mast Cells Trigger Inflammatory Cytokine Production by Human Airway Smooth Muscle Cells

Signaling Pathways in Exosomes Biogenesis, Secretion and Fate

Characterization of the proteins released from activated platelets leads to localization of novel platelet proteins in human atherosclerotic lesions

Contact Info

Product

Resources

About