Immunoresponses to Neisseria meningitidis epitopes: in vivo analysis of immunocompetent cells involved in suppression of secondary response to phosphorylcholine

Faro, José; Prado, Rafael Seoane; Lareo, I.; Eiras, A.; Schiller, Martin; Regueiro, Benito J.

doi:10.1007/bf00194889

Cited by 5 publications

(19 citation statements)

References 43 publications

(25 reference statements)

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“…If this is so, the simultaneous injection of PC-KLH plus another antigen able to elicit a good anti-PC response in aged mice might induce a normal response, because the other antigen would supply to B cells the appropriate activation signal. This experimental design had been previously used to study the mechanism underlying the suppression of anti-PC secondary response induced by NMB (8).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, responses induced in aged mice by coinjection of meningococcal antigens plus PC-KLH showed, in all cases, greater PC50, lower PFC numbers and lesser T15% and Hi values than those induced by the meningococcal antigen alone. On the contrary, when PC-KLH plus NMB were injected into NMB-primed mice, no interference was seen between both antigens and mice injected with the two antigens showed additive PFC responses (8), even more, the two antigens have additive responses when injected into untreated normal mice (25). As it would be very improbable that NMB enhanced PC-KLH responses without inducing any response itself, we think that the interpretation of a negative effect of PC-KLH on meningococcus responses, rather than the opposite explanation, i.e.…”

Section: Discussionmentioning

confidence: 99%

“…Abbreviations: BSA, bovine serum albumin; KLH, keyhole limpet hemocyanin; NMB, antigenic preparation of N. meningitidis group B; NZB/W, (NZB/NZW)F, mice; PC, phosphorylcholine; PC-KLH, PC coupled to KLH; PC-BSA, PC coupled to BSA; PC-(NMB)HI, PC coupled to heat-inactivated NMB; PFC, plaque forming cell; SS-T, 0.05% Tween-20 in saline solution; PBS-T-BSA, 0.05% Tween-20 in 0.5% BSA phosphate-buffered saline. PC50) a non-parametric method was used (4,8). The value of PC50 has been reported to be related to the secretion rate of antibody secreting cells rather than to the affinity or avidity of the antibody secreted (6).…”

Section: Methodsmentioning

confidence: 99%

“…Some antigenic preparations of N. meningitidis can induce a phosphorylcholine-specific response acting as a type 2 T-independent antigen (7) and can modulate this immunoresponse by causing carrier-specific suppression of the secondary response against natural and chemically bound meningococcal epitopes (7)(8)(9).…”

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confidence: 99%

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Interference between Neisseria meningitidis and PC‐KLH Induced Anti‐Phosphorylcholine PFC Responses in NZB/W Autoimmune Mice

Prado

Eiras

Quireza

et al. 1995

Microbiology and Immunology

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In this work, we demonstrate that the simultaneous injection of PC-KLH and Neisseria meningitidis-derived antigens [NMB or PC-(NMB)HI] induced in old NZB/W mice defective responses as does PC-KLH challenge. On the other hand, the simultaneous injection of both immunogenic preparations of N. meningitidis evoked responses similar to those shown by old mice challenged with NMB alone. Alteration in PC-specific PFC responses also affected hapten-free inhibition profiles and their heterogeneities. The increase in PC50s of anti-phosphorylcholine PFC responses and their heterogeneities induced by certain antigens with aging is correlated with a decrease in T15 idiotype expression, suggesting that after the T15 dominant clone disappears no other clone takes control of the anti-PC response. These results suggest that the mechanism(s) involved in the regulation of T15 marker expression play an important role in the inability of old NZB/W mice to mount good anti-PC responses and suggest that regulatory mechanisms induced by PC-KLH dominate those elicited by NMB.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Interference between Neisseria meningitidis and PC‐KLH Induced Anti‐Phosphorylcholine PFC Responses in NZB/W Autoimmune Mice

Prado

Eiras

Quireza

et al. 1995

Microbiology and Immunology

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“…Inoculation of mice with outer membrane vesicles from both encapsulated and non-encapsulated M986 strains has been shown to provide protection against lethal doses of endotoxin and infective doses of live bacteria (19). These strains have also been used to investigate immune regulatory properties of N. meningitidis (26,27) and both LOS and outer membrane vesicles enhance granulocyte recovery in myelosuppressed mice (22).…”

mentioning

confidence: 99%

The Fine Structure of Neisseria meningitidis Lipooligosaccharide from the M986 Strain and Three of Its Variants

Tsai

Jankowska-Stephens

Rahman

et al. 2009

Journal of Biological Chemistry

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Neisseria meningitidis is a cause of fatal sepsis and epidemic meningitis. A major virulence factor is cell wall lipooligosaccharide (LOS). The M986 strain has been used extensively in immunological and vaccine research. Yet, the LOS repertoire of this strain is not known. Here we have investigated the LOS structures of M986 and three of its variants OP1, OP2؊, and OP2؉. This strain and its variants present a series of related LOS families that are increasingly truncated in their listed order. The major structural differences are seen in the lacto-N-neotetraose ␣-chain. The ␥-chain Hep II contains two phosphoethanolamine (PEA) substitutions at C3 and C6/7. These substitutions were seen in all strains except OP2؉ where the canonical core Hep II is missing. The PEA disubstitution was present in nearly stoichiometric amounts with only minor amounts of monosubstitution observed, and no glycomers devoid of PEA were seen. This was also the case in LOS with a complete lacto-N-neotetraosyl ␣-chain even though previous reports suggested that the presence of an extended ␣-chain hinders C3 PEA substitution of Hep II. Approximately 50% of ␥-chain GlcNAc was present in its 3-OAc-substituted form. Because Hep II C3 PEA substitution and ␥-chain GlcNAc OAc addition have been reported to negatively interact, the co-existence of these two modifications in these strains is unique. The LOS structures of M986 and three of its variants have been determined, which better defines these strains as tools for immunological and vaccine research.

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Immunoresponses to Neisseria meningitidis epitopes: Immunodulation by meningococcus B acts on more than one meningococcal surface epitope

Faro

Prado

Lareo

et al. 1987

Med Microbiol Immunol

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Neisseria meningitidis group B strain M986 (serotypes 2a, 7) (NMB) elicits a specific primary antiphosphorylcholine immune response in mice but not a secondary response. The ability of other serotype and serogroup meningococci to induce similar primary responses in mice was studied, as was the immunogenicity of trinitrophenyl coupled NMB (TNP-NMB) in primary and secondary antitrinitrophenyl responses. Except for NMB, all other strains tested (three serogroup B and one serogroup A meningococcal strains) were found to be very poor phosphorylcholine immunogens. TNP-NMB, however, though proving to be a very good TNP antigen, was only a weak phosphorylcholine antigen. Priming NBF1 female mice with TNP-NMB one month or more before challenging them with the same antigen induced a strong depression of anti-TNP response in the subsequent challenge. However, this effect was not observed with Xid NBF1 male mice. Furthermore, priming mice with NMB weakly affected the anti-TNP response, but greatly depressed the antiphosphorylcholine response, after TNP-NMB challenge. In addition, whereas apparently only one TNP-specific B cell subpopulation was responding in unprimed mice challenged simultaneously with TNP-NMB and TNP-Ficoll (non-additive response), priming mice with NMB appeared to facilitate the independent activation of two different TNP-specific B cell subpopulations (additive response).

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Immunoresponses to Neisseria meningitidis epitopes: in vivo analysis of immunocompetent cells involved in suppression of secondary response to phosphorylcholine

Cited by 5 publications

References 43 publications

Interference between Neisseria meningitidis and PC‐KLH Induced Anti‐Phosphorylcholine PFC Responses in NZB/W Autoimmune Mice

Interference between Neisseria meningitidis and PC‐KLH Induced Anti‐Phosphorylcholine PFC Responses in NZB/W Autoimmune Mice

The Fine Structure of Neisseria meningitidis Lipooligosaccharide from the M986 Strain and Three of Its Variants

Immunoresponses to Neisseria meningitidis epitopes: Immunodulation by meningococcus B acts on more than one meningococcal surface epitope

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