Immunostaining of a cell type in the islets of Langerhans of the monkey Macaca irus by antibodies against S-100 protein

Abstract

S-100 protein-immunoreactive cells were demonstrated by immunocytochemical procedures in the pancreatic islets of Langerhans in the monkey Macaca irus. By use of antibodies against human S-100 protein or bovine S-100 protein, these cells were observed in all islets in the head and tail portions of the pancreas. Immunostained cells were usually located in the center of the islets or sometimes found in a more widely distributed form, but they were never arranged in a regular concentric fashion. The number of immunoreactive cells varied from one islet to another but it was relatively limited making up only 0.75%-6.3% of all insular cells. With the use of the double-immunoenzymatic procedure for demonstration of the four main endocrine cell types (insulin-, glucagon-, somatostatin- and pancreatic polypeptide producing elements), it was possible to establish that S-100 protein-immunoreactive cells represent a distinct cell type. Antibodies against S-100 protein-stained neuroinsular complexes. The present findings speak in favor of a new cell type to be added to the large variety of S-100 protein-immunoreactive cells outside the central nervous system.

Human odontoblasts contain S‐100 protein‐like immunoreactivity

“…Until recently, S-100 protein was considered a specific marker for neural tissue. Investigations based on immunohistochemistry have also shown that S-100 proteins are not specific for neural tissue but are widely present among animal and human cells such as chondrocytes (Stefansson et al, 1982), endocrine cells (Girod et al, 1987;Zabel and Dietel, 1987), reticular cells of lymphoid tissue, and in their neoplastic counterparts (Takahashi et al, 1981;Nakajima et al, 1984). S-100b has also been isolated from adipose cells [Hidaka et ai., 19831.…”

S‐100 immunoreactive cells in non‐nervous duck tissues

“…Furthermore, there was disagreement of cell types containing S-100 protein in avian and mammalian tissues; for example, in the islets of Langerhans of the duck it was suggested that B cells and D cells contain S-100 protein, because all cells of the beta islet were immunoreactive for this protein and in the alpha islets, S-100-protein-positive cells coincide with argyrophilic cells. In the islet of Langerhans of the monkey, on the other hand, S-100-protein-positive cells represent a new type of cell differing from insulin-, glucagon-, somato statin-and pancreatic-peptide cells (Girod et al, 1987).…”

<b>IMMUNOHISTOCHEMICAL DEMONSTRATION OF S-100 PROTEIN IN THE ENDOTHELIAL CELLS OF BLOOD VESSELS IN THE PIG AND </b><b>CATTLE </b>

“…The immunoreactivity for S-100 protein has been reported not only in some neurons, but also in mesenchymal cells such as chondrocytes, fat cells, some fibroblastic cells, lymphocytes, macrophages and Langerhans cells (17,23). Some ductal cells of exocrine glands (l5), endocrine cells (4,28) and urinary tubules (14, l9) have been claimed to show S-100 immunoreactivity. Contractile cells including cardiocytes, skeletal muscle cells and myoepithelial cells (9,17) have also been shown to be immunoreactive for S-100 protein.…”