Immunostick Colorimetric ELISA Assay for the Identification of Smoked Salmon, Trout and Bream

Carrera, Esther; Garcı́a, Teresa; Céspedes, Ana; González, Isabel; Sanz, B.; Hernández, Pablo E.; Martı́n, Rosario

doi:10.1002/(sici)1097-0010(199708)74:4<547::aid-jsfa828>3.0.co;2-q

Cited by 38 publications

(9 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is therefore important the setting up of fast, reliable, reproducible and low cost techniques to be applied to test the authenticity of the bivalve species that are being used as raw material in order to avoid economic fraud. To achieve this goal, several techniques based on protein analysis (electrophoretic, chromatographic and immunological methods) have been developed for fish species identification (Carrera et al, 1997;Mackie et al, 2000). However according to Lockley and Bardsley (2000) those methodologies have some limitations especially in processed products, where processing techniques may easily result in protein denaturation.…”

Section: Introductionmentioning

confidence: 99%

Identification of the wedge clam Donax trunculus by a simple PCR technique

Pereira¹,

Fernández‐Tajes²,

Gaspar³

et al. 2012

Food Control

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Identification of the wedge clam Donax trunculus by a simple PCR technique

Pereira¹,

Fernández‐Tajes²,

Gaspar³

et al. 2012

Food Control

View full text Add to dashboard Cite

“…Bibliography accounts for some research about its biology and fishery [17,18], but little information has been provided about changes produced in this fish species as a result of processing [19]. The possibility of replacing higher cost species by Atlantic pomfret during the commercialisation of smoked products has developed some species identification research [20,21].…”

Section: Introductionmentioning

confidence: 99%

Shelf life extension of Atlantic pomfret ( Brama brama ) fillets by packaging under a vacuum-skin system

Pérez‐Alonso

Aubourg

Rodrı́guez

et al. 2004

European Food Research and Technology

View full text Add to dashboard Cite

SUMMARYLipid damage produced during Atlantic pomfret (Brama brama) chilled storage (up to 19 days) was studied. For it, free fatty acid (FFA) and conjugated diene (CD) formation, peroxide value (PV), thiobarbituric acid reactive substances and fluorescent compounds formation were measured in two different white muscle zones (dorsal and ventral). Comparison with volatile amine formation (total volatile base-nitrogen, TVB-N; trimethylamine-nitrogen, TMA-N) was carried out. A gradual lipid hydrolysis was observed in both zones along the whole experiment. CD and peroxides formation was important during the experiment; peroxides showed the highest value at day 15 in both zones, that was followed by a peroxide breakdown at day 19. Interaction of oxidized lipids with nucleophilic compounds present in the fish muscle led to a gradual fluorescence development during the storage time in both zones. Mean values of the lipid content showed to be higher in the ventral zone than in the dorsal one, although significant differences could not be detected (p<0.05). Concerning the lipid damage, very small differences (p>0.05) could be assessed between both zones. TVB-N content showed no variation till day 15. At day 19, a sharp increase was observed for both muscle zones. In the case of TMA, a gradual increase was observed along the whole storage time for both muscle zones. Higher mean values were obtained in TVB-N and TMA-N indices for the ventral zone than for the dorsal one, according to a closer location to viscera where microorganisms are known to concentrate. Among the different indices tested for quality assessment, TMA-N and fluorescence formation were the most accurate for following the quality loss during Atlantic pomfret chilling, showing satisfactory (r 2 ≥0.87) non-linear fittings with the chilled storage time.2

show abstract

“…The antibodyantigen blocking technique (Jones & Patterson, 1986) is a simple method in which the proteins that cross-react with the antiserum are used to block the antibodies directed against them. This procedure has been widely used in immunoassays to remove cross-reactions (Jones & Patterson, 1986;Garcṍ a et al, 1990;Taylor & Jones, 1992;Taylor et al, 1994;Carrera et al, 1997). Blocking with antigenic extracts from the heterologous clam species, clearly improved the specify of the anti-DSP, anti-RSP and anti-PSP antibodies.…”

Section: Resultsmentioning

confidence: 99%

Identification of the Clam Species Ruditapes decussatus (Grooved Carpet Shell), Venerupis rhomboides (Yellow Carpet Shell) and Venerupis pullastra (Pullet Carpet Shell) by ELISA

Fernández¹,

Garcı́a²,

Asensio³

et al. 2002

Food and Agricultural Immunology

Self Cite

View full text Add to dashboard Cite

An ELISA has been developed for the identification of three clam species: Ruditapes decussatus (grooved carpet shell), Venerupis rhomboides (yellow carpet shell) and Venerupis pullastra (pullet carpet shell). The assay was performed using polyclonal antibodies (PAbs) against clam soluble proteins in two different formats: microtiter plates and immunostick tubes. PAbs were rendered species-specific by blocking them with soluble proteins from heterologous clam species. For the indirect ELISA, clam soluble proteins were adsorbed onto polystyrene plates or immunostick paddles. Species-specific PAbs were then added to the wells or tubes. Goat anti-rabbit immunoglobulins conjugated to the enzyme horseradish peroxidase were used for the immunorecognition of the PAbs adsorbed to clam samples. Subsequent enzymatic conversion of the substrate allowed the identification of the three clam species analyzed.

show abstract

Immunostick Colorimetric ELISA Assay for the Identification of Smoked Salmon, Trout and Bream

Cited by 38 publications

References 8 publications

Identification of the wedge clam Donax trunculus by a simple PCR technique

Identification of the wedge clam Donax trunculus by a simple PCR technique

Shelf life extension of Atlantic pomfret ( Brama brama ) fillets by packaging under a vacuum-skin system

Identification of the Clam Species Ruditapes decussatus (Grooved Carpet Shell), Venerupis rhomboides (Yellow Carpet Shell) and Venerupis pullastra (Pullet Carpet Shell) by ELISA

Contact Info

Product

Resources

About