Immunostimulatory Activities of Coliphages on In Vitro Activated Mammalian Macrophages

Yıldızlı, Gizem; Coral, Gökhan; Ayaz, Furkan

doi:10.1007/s10753-019-01140-9

Cited by 10 publications

(10 citation statements)

References 34 publications

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“…E. casseliflavus ATCC 700327, E. coli ATCC 25922, P. aeruginosa ATCC 27853, S. aureus ATCC, A. niger ATCC 16888, C. parasitica , P. cinnamomi , P. tracheiphilus , and two coliphages (P1, P2) that we isolated in our previous study, [17] were used in this study. P. tracheiphilus , C. parasitica , and P. cinnamomi were obtained from diseased host plants, and each species was confirmed based on morphological features of the colony grown on Potato Dextrose Agar (PDA, Neogen).…”

Section: Methodsmentioning

confidence: 99%

“…RAW 264.7 (ATCC) mammalian macrophage like cells were utilized in our experiments. Experimental procedures that were described by our previous publications [17] but briefly, the cells were grown in 37 °C 5 % CO 2 incubator by using complete RPMI media (RPMI 1640+10 % Fetal Bovine Serum+1 % Streptomycin/Penicillin. The cells were plated into the 24‐well plates in 106 cells/mL concentration and with a final volume of 1 mL.…”

Section: Methodsmentioning

confidence: 99%

“…Bacteriophages were also used in this study. We utilized from coliphages (P1, P2) that were isolated in our previous work [17] . Bacteriophages are used in agriculture and animal husbandry to specifically eliminate harmful bacteria [18,19] .…”

Section: Introductionmentioning

confidence: 99%

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Biochar as a Biocompatible Mild Anti‐Inflammatory Supplement for Animal Feed and Agricultural Fields

Yıldızlı

Coral

Ayaz

2021

Chemistry & Biodiversity

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Biochar is an organic material and high in carbon content, besides its use for energy purposes, it is also a material that serves the purpose of improving soil fertility, organic matter content of soils and removing heavy metals from water and soil. This study aims to investigate the antimicrobial effects of biochar whose beneficial effects on agricultural productivity has been proven by different studies. Scientific literature concerning the antibacterial, antifungal, and antiviral effects of the apricot seed and olive seed biochar is limited. Biochar applications may help to alter the microbial diversity by modifying biological environment either in agriculture or in animal husbandry. Moreover, biochar has been used in animal husbandry to improve animal health especially by regulating the intestinal flora and inflammation in the intestines. Hence, in our study, we investigated the effect of biochar on the growth of Aspergillus niger, Cryphonectria parasitica, Phytophthora cinnamomi, Plenodomus tracheiphilus, Enterococcus casseliflavus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and two different bacteriophage strains. Biochar did not have any direct effect on the growth of either Gram‐positive or Gram‐negative bacteria, bacteriophages, and fungi. In order to test their direct effects on the immune cells, mammalian macrophages were used and biochar directly reduced the inflammatory cytokine levels produced by the in vitro activated macrophages.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Biochar as a Biocompatible Mild Anti‐Inflammatory Supplement for Animal Feed and Agricultural Fields

Yıldızlı

Coral

Ayaz

2021

Chemistry & Biodiversity

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“…Yıldızlı et al [63] tested the effects of two bacteriophages infecting E. coli on the activation status of mammalian macrophages and TNF-α levels. The bacteriophages were able to effectively activate macrophages to produce TNF-α in the absence of lipopolysaccharide (LPS), which is a bacterial stimulant of the inflammatory response.…”

Section: Monocytes and Macrophagesmentioning

confidence: 99%

“…This indicated that under these conditions, there were no effects of LPS on macrophage phagocytic potential. The most probable explanation for this phenomenon was the activation of macrophages by bacteriophages through a signaling pathway different than LPS [63].…”

Section: Monocytes and Macrophagesmentioning

confidence: 99%

Interactions of Bacteriophages with Animal and Human Organisms—Safety Issues in the Light of Phage Therapy

Podlacha

Grabowski

Kosznik-Kawśnicka

et al. 2021

IJMS

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Bacteriophages are viruses infecting bacterial cells. Since there is a lack of specific receptors for bacteriophages on eukaryotic cells, these viruses were for a long time considered to be neutral to animals and humans. However, studies of recent years provided clear evidence that bacteriophages can interact with eukaryotic cells, significantly influencing the functions of tissues, organs, and systems of mammals, including humans. In this review article, we summarize and discuss recent discoveries in the field of interactions of phages with animal and human organisms. Possibilities of penetration of bacteriophages into eukaryotic cells, tissues, and organs are discussed, and evidence of the effects of phages on functions of the immune system, respiratory system, central nervous system, gastrointestinal system, urinary tract, and reproductive system are presented and discussed. Modulations of cancer cells by bacteriophages are indicated. Direct and indirect effects of virulent and temperate phages are discussed. We conclude that interactions of bacteriophages with animal and human organisms are robust, and they must be taken under consideration when using these viruses in medicine, especially in phage therapy, and in biotechnological applications.

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Ruthenium Bipyridyl Dithiocyanate Complex Exerted Adjuvant Activity on the Activated Mammalian Macrophages in vitro

Ayaz

2020

Inflammation

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Immunostimulatory Activities of Coliphages on In Vitro Activated Mammalian Macrophages

Cited by 10 publications

References 34 publications

Biochar as a Biocompatible Mild Anti‐Inflammatory Supplement for Animal Feed and Agricultural Fields

Biochar as a Biocompatible Mild Anti‐Inflammatory Supplement for Animal Feed and Agricultural Fields

Interactions of Bacteriophages with Animal and Human Organisms—Safety Issues in the Light of Phage Therapy

Ruthenium Bipyridyl Dithiocyanate Complex Exerted Adjuvant Activity on the Activated Mammalian Macrophages in vitro

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