Immunostimulatory Activity of Chitosan Nanoparticles on Wistar Albino Rats

Sudjarwo, Giftania Wardani; Mahmiah, Mahmiah; Sudjarwo, Sri Agus

doi:10.5530/pj.2018.5.150

Cited by 14 publications

(13 citation statements)

References 19 publications

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“…and it decreased subsequently, that result disagreed with the present study in which the skin thickness reached the maximum at the 48 hrs of injection. Wardani et al (17) found that chitosan nanoparticles induce an increase in DTH response in rats, shows a stimulatory effect of the chitosan nanoparticles which has happened on the lymphocytes and accessory cell types essential for the expression of this reaction and this with an agreement with the present study that showed gradual increase in induration of skin thickness. Since both LLO and chitosan have an immunostimulatory effect and can induce cell-mediated immunity and the activated T cells produce cytokines and chemokines, which attract macrophages and cytotoxic CD8+ T cells may explain the elevated mean value (P≤0.05) skin thickness in the CS+LLO group as compared to the CS and LLO group as mentioned by Carrero et al and Li et al (18,19).…”

Section: Discussionsupporting

confidence: 93%

Immunotherapeutic effect of chitosan and listeriolysin O on Listeria monocytogenes infection in mice

Ali¹,

Yasssein²

2021

IJVS

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This study aimed to evaluate the effect of dietary chitosan and listeriolysin O (LLO) on the immune response against Listeria monocytogenes infection in mice. L. monocytogenes isolate was obtained from the unit of Zoonotic Disease, College of Veterinary Medicine, University of Baghdad. It was reactivated and cultured on PALCAM agar and exposed to a confirmatory diagnostic test. listeriolysin O (LLO) was extracted and purified. Sixty mice were used and divided into four groups each one involving 15 mice; the first group was fed on diet supplemented with a chitosan for 4 weeks. At the end of the 2 nd week, normal saline was injected S/C; the second group was fed on a normal diet without any addition. At the end of 2 nd week, 0.3 ml of LLO was injected S/C, then the booster dose of LLO was given after 14 days of 1 st injection; the third group was fed on a diet supplemented with chitosan as in G1, and treated as in G2 and the fourth group fed on a normal diet without any addition and treated as in G1. A skin test was performed on 5 mice of each group while a Challenge test by injection of 0.2ml of 1x10 9 CFU/ml of viable L. monocytogenes intraperitoneally was performed on other mice. The concentration of IgG titer and IL6 were measured. The results revealed that the third group recorded significantly higher values in the skin thickness, IgG, and IL6 concentrations when compared with other groups at different times which indicate that LLO and chitosan may support each other to provide the most beneficial effect by eliciting of good cellular and humeral immunity against listeriosis.

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Section: Discussionsupporting

confidence: 93%

Immunotherapeutic effect of chitosan and listeriolysin O on Listeria monocytogenes infection in mice

Ali¹,

Yasssein²

2021

IJVS

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“…Moreover, COS is a potent oxygen-free radical scavenger and has the ability to bind to divalent cations, including Cd (KIM et al, 2016;NAVEED et al, 2019). WARDANI and SUDJARWO (2018) reported that chitosan nanoparticles show immunostimulatory activity and have therapeutic potential for some immune depressed conditions. In addition, GUO et al (2018) also showed the effect of COS on some hematological parameters in humans.…”

Section: Methodsmentioning

confidence: 99%

The influence of chitosan oligosaccharide on some hematological parameters in rats exposed to cadmium

Kısadere¹,

Aydın²,

Dönmez³

2022

Vet. arhiv

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The aim of the study was to investigate the effects of chitosan oligosaccharide (COS) on some hematological parameters, and define the percentage of alpha-naphthyl acetate esterase (ANAE) positive lymphocytes in rats that had been exposed to cadmium (Cd). Thirty-two (n = 32) male Wistar albino rats were randomly divided into four groups as the control (C), chitosan oligosaccharide (COS), cadmium (Cd), and Cd + COS (CdCOS) groups. Blood samples were collected to assess erythrocytes (RBC), leukocytes (WBC), hemoglobin levels (HGB), hematocrit values (HCT), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV), platelets (PLT), and alpha-naphthyl acetate esterase (ANAE) positive lymphocytes. The number of WBCs significantly increased in the Cd group compared to the C, CdCOS, and COS groups (P<0.05). Although the lymphocyte count decreased significantly in the Cd group (P<0.05), it was ameliorated by COS treatment in the CdCOS group compared to the Cd group (P<0.05). Also, the percentage of peripheral blood ANAE positive lymphocytes decreased significantly in the CdCOS and Cd groups (P<0.05). COS exhibits a partially protective effect on some hematological characteristics, apart from the percentage of ANAE positive lymphocyte in cases of chronic Cd exposure.

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“…Compared to chitosan oligosaccharides, the chitosan nanoparticles showed an enhanced immunomodulatory effect (1.2–1.5-fold), indicating the immunomodulatory effect could be tuned by aggregation of nanoparticles [ 211 ]. Wardani et al [ 212 ] also applied chitosan nanoparticles to animal models and concluded that these nanoparticles could effectively stimulate immune responses and have a therapeutic potential for immunotherapy.…”

Section: Chitosan-based Nanomedicinesmentioning

confidence: 99%

Polysaccharide-based nanomedicines for cancer immunotherapy: A review

Zeng

Xiang

Sheng

et al. 2021

Bioactive Materials

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Cancer immunotherapy is an effective antitumor approach through activating immune systems to eradicate tumors by immunotherapeutics. However, direct administration of “naked” immunotherapeutic agents (such as nucleic acids, cytokines, adjuvants or antigens without delivery vehicles) often results in: (1) an unsatisfactory efficacy due to suboptimal pharmacokinetics; (2) strong toxic and side effects due to low targeting (or off-target) efficiency. To overcome these shortcomings, a series of polysaccharide-based nanoparticles have been developed to carry immunotherapeutics to enhance antitumor immune responses with reduced toxicity and side effects. Polysaccharides are a family of natural polymers that hold unique physicochemical and biological properties, as they could interact with immune system to stimulate an enhanced immune response. Their structures offer versatility in synthesizing multifunctional nanocomposites, which could be chemically modified to achieve high stability and bioavailability for delivering therapeutics into tumor tissues. This review aims to highlight recent advances in polysaccharide-based nanomedicines for cancer immunotherapy and propose new perspectives on the use of polysaccharide-based immunotherapeutics.

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Immunostimulatory Activity of Chitosan Nanoparticles on Wistar Albino Rats

Cited by 14 publications

References 19 publications

Immunotherapeutic effect of chitosan and listeriolysin O on Listeria monocytogenes infection in mice

Immunotherapeutic effect of chitosan and listeriolysin O on Listeria monocytogenes infection in mice

The influence of chitosan oligosaccharide on some hematological parameters in rats exposed to cadmium

Polysaccharide-based nanomedicines for cancer immunotherapy: A review

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