Immunostimulatory activity of glycopeptides from Paecilomyces sinensis under normal and cyclophosphamide induced immunosuppressive conditions in mice models

Abstract

The present study was designed to evaluate immune-modulating effects of the glycopeptide from Paecilomyces sinensis (CPS-II) by using mouse peritoneal macrophage and cytoxan (CTX) induced immunosuppression models. Our results from phagocytotic and mononuclear phagocytic system function assays showed that CPS-II stimulated phagocytosis of the phagocytes. A splenocyte proliferation assay showed that CPS-II acted to combine Concanavalin A (ConA) or lipopolysaccharides (LPS) in splenocyte proliferation. The results demonstrated that CPS-II increased the indices of the thymus and spleen. Hematological and histopathological analysis revealed the protective effect of CPS-II against CTX induced immunosuppression. CPS-II also significantly increased the expression of CD4(+) and CD8(+) splenic T lymphocytes, which were suppressed by CTX in peripheral blood. The expressions of serum cytokines related to immune function, including TNF-α, IL-6, and IFN-γ, were up-regulated in a dose-dependent manner. The expression of the transcription factor NF-κB in the spleen was enhanced after CPS-II-treatment. In conclusion, our results indicated that CPS-II was involved in immunostimulatory actions leading to its modulatory effects on immunosuppression, and one possible mechanism of action was to activate NF-κB.

Treatment with Subcritical Water-Hydrolyzed Citrus Pectin Ameliorated Cyclophosphamide-Induced Immunosuppression and Modulated Gut Microbiota Composition in ICR Mice

“…Thus, Cy was used to creating the immunosuppressed animal model in this study. Cy-induced immunosuppressed mice exhibited significantly lower body weight gain, spleen index, and cytokine levels (IFN-γ, IL-2, IL-6, and TNF-α) compared to the control group [24]. Similar changes were also shown in the present Cy-induced mouse model, indicating that the immunosuppression model was built successfully.…”

Immunostimulatory effects of cordycepin‐enriched WIB‐801CE from Cordyceps militaris in splenocytes and cyclophosphamide‐induced immunosuppressed mice

“…The spleen is an important immune organ in the body and an important place of differentiation of the thymocytes in the lymphocyte (Z. Y. Zhu, Meng, Sun, et al, ). These lymphocytes are the major effector cells in the mammalian immune system and a component of cell‐mediated response.…”

Kanggan Granule Ameliorates Dexamethasone-Induced Immunosuppression in Mice

“…The spleen contains a large number of lymphocytes, including B-lymphocytes and T-lymphocytes, which play an important role in humoral and cellular immunity. We investigated the proliferative capacity of B-and T-lymphocytes that were stimulated by LPS and ConA to evaluate changes in the immune response [22]. The results showed that KG effectively enhanced the proliferative capacity The results of KG-24 administration were not as promising as those of KG-6.…”