Immunostimulatory effect of a marine yeast Candida sake S165 in Fenneropenaeus indicus

Puthiyedathu, Sajeevan Thavarool; Philip, Rosamma; Singh, I. S. Bright

doi:10.1016/j.aquaculture.2006.03.008

Cited by 61 publications

(35 citation statements)

References 19 publications

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“…The two marine yeasts, C. haemulonii S27 and C. sake S 165 have been proved to be good source of immunostimulants by Prabha (2007) and Sajeevan et al (2006), respectively. Bacillus and Micrococcus are commercial probionts used in shrimp culture (Antony and Philip 2008).…”

Section: Immunostimulants/probiotics Usedmentioning

confidence: 99%

“…Group 2 was fed the experimental diet CHG (0.2% C. haemulonii glucan) and group 3, CSG (0.2% C. sake S165 glucan). Group 4 was fed experimental diet CHY (10% C. haemulonii S27 biomass) and group 5, CSY (10% C. sake S165 biomass) (Sajeevan et al 2006). Group 6, 7, and 8 were fed experimental feeds B (Bacillus MCCB101 incorporated diet (50 cells/g animal/day)); M (Micrococcus MCCB104 (50 cells/g animal/day)) and BM (Bacillus MCCB101 + Micrococcus MCCB104 (100 cells/g animal/day)), respectively.…”

Section: Feeding Experiments and Wssv Challengementioning

confidence: 99%

“…Seven different types of experimental diets were prepared: i.e., two glucan diets (CHG and CSG), two yeast diets (CHY and CSY) and three probiont incorporated diets (B, M and BM). The two glucan diets (CHG and CSG) were prepared by incorporating 0.2% glucan (extracted from C. haemulonii S27 and C. sake S165) with the standard diet based on previous studies (Sajeevan et al 2006(Sajeevan et al , 2009. For yeast diets (CHY and CSY), the two marine yeast (wet weight) biomass (C. haemulonii S27and C. sake S165) were incorporated at a concentration of 10% (w/w) in the standard diet.…”

Section: Experimental Diets Usedmentioning

confidence: 99%

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Molecular characterization of a crustin-like antimicrobial peptide in the giant tiger shrimp, Penaeus monodon, and its expression profile in response to various immunostimulants and challenge with WSSV

et al. 2011

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a b s t r a c tA crustin-like antimicrobial peptide from the haemocytes of giant tiger shrimp, Penaeus monodon was partially characterized at the molecular level and phylogenetic analysis was performed. The partial coding sequence of 299 bp and 91 deduced amino acid residues possessed conserved cysteine residues characteristic of the shrimp crustins. Phylogenetic tree and sequence comparison clearly confirmed divergence of this crustin-like AMP from other shrimp crustins. The differential expression of the crustin-like AMP in P. monodon in response to the administration of various immunostimulants viz., two marine yeasts (Candida haemulonii S27 and Candida sake S165) and two ␤-glucan isolates (extracted from C. haemulonii S27 and C. sake S165) were noted during the study. Responses to the application of two gram-positive probiotic bacteria (Bacillus MCCB101 and Micrococcus MCCB104) were also observed. The immune profile was recorded pre-and post-challenge white spot syndrome virus (WSSV) by semi-quantitative RT-PCR. Expressions of seven WSSV genes were also observed for studying the intensity of viral infection in the experimental animals. The crustin-like AMP was found to be constitutively expressed in the animal and a significant down-regulation could be noted post-challenge WSSV. Remarkable down-regulation of the gene was observed in the immunostimulant fed animals pre-challenge followed by a significant up-regulation post-challenge WSSV. Tissue-wise expression of crustin-like AMP on administration of C. haemulonii and Bacillus showed maximum transcripts in gill and intestine. The marine yeast, C. haemulonii and the probiotic bacteria, Bacillus were found to enhance the production of crustin-like AMP and confer significant protection to P. monodon against WSSV infection.

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Section: Immunostimulants/probiotics Usedmentioning

confidence: 99%

Section: Feeding Experiments and Wssv Challengementioning

confidence: 99%

Section: Experimental Diets Usedmentioning

confidence: 99%

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Molecular characterization of a crustin-like antimicrobial peptide in the giant tiger shrimp, Penaeus monodon, and its expression profile in response to various immunostimulants and challenge with WSSV

et al. 2011

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“…Improved antigen delivery and protective responses in carp administered BF cells of A. hydrophila by oral route have been demonstrated (Azad et al 2000). Elevated THC and PO activity were also reported when shrimp were administered various immunostimulants like probiont bacterium (Rengpipat et al2000), b-1, 3-glucan (Lopez et al 2003), spent brewer's yeast b-glucan (Suphantharika et al 2003), chitin/chitosan (Wang and Chen 2005), Sargassum fusiforme polysaccharide extracts (Huang et al 2006) and marine yeast (Sajeevan et al 2006).…”

Section: Resultsmentioning

confidence: 99%

Development of biofilm of Vibrio alginolyticus for oral immunostimulation of shrimp

et al. 2010

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Biofilm (BF) of Vibrio alginolyticus was developed on chitin flakes. BF formation was studied at various nutrient concentrations and incubation time. Highest colonyforming units of BF cells were obtained with 0.15% trypticase soya broth and at 3 days of incubation. The BF cells could be completely inactivated at 80°C in 10 min and with 10% formalin in 24 h. SDS-PAGE profile of BF cells revealed repression of four proteins and expression of three new proteins compared to free cells (FC). The preliminary immune response studies showed that BF cells were superior to FC in stimulating the non-specific immune response of Penaeus monodon.

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“…One of the benefits of adding yeast to shrimp diets is its high content of β-glucan, which improves resistance against diseases (Chang et al, 2003;Sajeevan et al, 2006;Song et al, 1997;Sung et al, 1994;). For example, Sajeevan et al (2009) obtained higher survival and immune response in the Indian white prawn Fenneropenaeus indicus challenged with WSSV after 40 days with a diet containing 10% whole cell biomass of the marine yeast Candida sake S165 and a diet with 0.2% (w/w) β-glucan from the same yeast, compared to the control diet.…”

Section: Discussionmentioning

confidence: 99%

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Sánchez¹,

Nolasco-Soria²,

Peña‐Rodríguez³

et al. 2018

Turk. J. Fish. Aquat. Sci.

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Marine yeasts used in aquaculture disease control can also be an important protein source for improving feeding and nutrition of crustaceans. Yarrowia lipolyticca has been studied for its capacity to secrete heterologous proteins and high content of unsaturated fatty acids, beta-glucan, and mannane polymers in the cell wall. We measured in vitro digestibility of Y. lipolyticca by whiteleg shrimp Litopenaeus vannamei digestive enzymes, and an in vivo assay of Y. lipolytica in feed on whiteleg shrimp growth. We found that digestive gland enzymes of shrimp digest Y. lipolytica, based on reduced optical density of a yeast suspension. Digestion was -0.00236 ± 0.00010 OD U min ) was measured. Digestive gland treatment significantly reduced cell viability (near 100%), relative to the control. Electron microscopy shows that the cell wall of Y. lipolytica exposed to the digestive gland enzymes was severely damaged. Shrimp diet containing Y. lipolytica resulted in significantly higher weight gain and specific growth rate of whiteleg shrimp.

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Immunostimulatory effect of a marine yeast Candida sake S165 in Fenneropenaeus indicus

Cited by 61 publications

References 19 publications

Molecular characterization of a crustin-like antimicrobial peptide in the giant tiger shrimp, Penaeus monodon, and its expression profile in response to various immunostimulants and challenge with WSSV

Molecular characterization of a crustin-like antimicrobial peptide in the giant tiger shrimp, Penaeus monodon, and its expression profile in response to various immunostimulants and challenge with WSSV

Development of biofilm of Vibrio alginolyticus for oral immunostimulation of shrimp

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