Immunostimulatory effect of pepsin enzymatic extract from <scp><i>Porphyra yezoensis</i></scp> on murine splenocytes

Herath, Kalahe Hewage Iresha Nadeeka Madushani; Lee, Jeong Ha; Cho, Jin-Hee; Kim, Areum; Shin, Sumin; Kim, Bohyung; Jeon, You‐Jin; Jee, Youngheun

doi:10.1002/jsfa.8851

Cited by 5 publications

(3 citation statements)

References 35 publications

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“…In a case-control study of breast cancer in South Korea, Yang et al (2010) reported the daily intake of several species of Pyropia (as Porphyra), to be inversely associated with the risk of developing cancer. Pyropia ('gim' in Korea) is commonly eaten dried and roasted (Herath et al 2018;Jung & Choe 2017). A cohort of 362 women with histologically confirmed breast cancer (aged 30-65), and 362 control participants of equal age and menopausal status, completed a food frequency questionnaire for 12 months containing 121 food items, including seaweed.…”

Section: Cancermentioning

confidence: 99%

Seaweeds as nutraceuticals for health and nutrition

2019

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Throughout human history, seaweeds have been used as food, folk remedies, dyes, and mineral-rich fertilisers. Seaweeds as nutraceuticals or functional foods with dietary benefits beyond their fundamental macronutrient content, are now a major research and industrial development concept. The occurrence of dietary and lifestyle-related diseases, notably type 2 diabetes, obesity, cancer, and metabolic syndrome has become a health epidemic in developed countries. Global epidemiological studies have shown that countries where seaweed is consumed on a regular basis have significantly fewer instances of obesity and dietary-related disease. This review outlines recent developments in seaweed applications for human health from an epidemiological perspective and as a functional food ingredient.

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Section: Cancermentioning

confidence: 99%

Seaweeds as nutraceuticals for health and nutrition

2019

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“…It contains a diverse repertoire of immune cells such as macrophages, dendritic cells, T and B lymphocytes and more importantly, both innate and adaptive immune processes appear in it, rendering immune reactions in splenocytes a reliable surrogate of the immune responses in the host in general (Wluka & Olszewski, 2006). A recent study also found that Porphyra yezoensis enzymatic extract increases the proliferation of murine splenocytes (Herath et al, 2018).…”

Section: Discussionmentioning

confidence: 88%

Immunomodulatory properties of enzymatic extract of Stichopus japonicus on murine splenocytes

Mihindukulasooriya¹,

Kim²,

Dinh³

et al. 2021

Fish Aquat Sci

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Red sea cucumber Stichopus japonicus is an invertebrate animal inhabiting in coasts of Korea, China, and Japan. They are traditionally used for food and medicine and well known for their distinctive biologically and pharmacologically important compounds. We investigated the effect of amyloglucosidase (AMG) enzymatic extracts of S. japonicus (AESJ) on the proliferation and cytokine secretion of murine splenocytes stimulated with concanavalin A (Con A). AESJ enhanced the proliferation of splenocytes and the production of IL-2 (Th1 cytokine), IL-1β (Th1 promoting cytokine), and IL-4, IL-10 (Th2 type cytokines) when treated alone. However, under Con A stimulation, AESJ suppressed the proliferation of splenocytes, attenuated the secretion of IL-2, IL-4, IL-10, and enhanced IL-1β secretion. These results suggest that AESJ exhibits immunomodulatory effect by moderating the proliferation of splenocytes and the secretion of IL-2, IL-1β, IL-4, and IL-10 differently depending on the absence and presence of Con A stimulation. These data evidence the immunomodulatory potential of AESJ, which can be further developed into a functional food mediating homeostasis.

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“…The modulation of cell proliferation by MC and/or PM was measured using 3 H-thymidine incorporation assay as previously described [ 47 ]. MLE-12 cells (1 × 10 4 cells/well) seeded in triplicates on 96-well plates (Nunc) were exposed to either varying concentrations (0, 3.9, 7.8, 15.6, 31.3, 62.5, 125, and 250 µg/mL) of MC or PM alone or varying concentrations of PM together with selected concentrations of MC (0, 31.3, and 62.5 µg/mL) for 54 h. After adding 1 μCi of 3 H-thymidine (42 Ci/mmol specific activity), cells were incubated for 18 h, and then harvested on glass fiber filters to determine the amount of thymidine incorporated into DNA using a liquid scintillation spectrometer (Wallac Micro Beta ® TriLux, Perkin Elmer, Waltham, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Mojabanchromanol Isolated from Sargassum horneri Attenuates Particulate Matter Induced Inflammatory Responses via Suppressing TLR2/4/7-MAPK Signaling in MLE-12 Cells

et al. 2020

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Chromanols from marine algae are studied for drug development due to its prominent bioactive properties, and mojabanchromanol (MC), a chromanol isolated from a brown algae Sargassum horneri, is found to possess anti-oxidant potential. In this study, we hypothesized MC may attenuate particulate matter (PM)-induced and reactive oxygen species (ROS)-mediated inflammatory responses in airways and tried to identify its potential and underlying mechanism against PM (majority <2.5 µm in diameter)-induced inflammatory responses in a lung type II alveolar epithelial cell line, MLE-12. MC attenuated PM-induced malondialdehyde (MDA), a lipid peroxidation end product, and 8-hydroxydeoxyguanosine (8-OHdG), the most representative DNA oxidative damage product, further validating MC’s potential in attenuating PM-induced oxidative stress. MC also suppressed PM-triggered TLR2/4/7 activation in MLE-12 cells. Moreover, MC reduced ROS-mediated phosphorylation of mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (Erk1/2) and c-Jun NH (2)-terminal kinase (JNK) that were also activated in PM exposed cells. MC further inhibited the secretion of pro-inflammatory cytokines (IL-6, IL-1β and IL-33) in MLE-12 cells exposed to PM. These results provide a clear evidence for MC’s potential in attenuating PM-triggered inflammatory responses in MLE-12 cells via repressing TLR2/4/7 and MAPK signaling. Therefore, MC can be developed as a therapeutic agent against PM induced airway inflammatory responses.

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Immunostimulatory effect of pepsin enzymatic extract from Porphyra yezoensis on murine splenocytes

Cited by 5 publications

References 35 publications

Seaweeds as nutraceuticals for health and nutrition

Seaweeds as nutraceuticals for health and nutrition

Immunomodulatory properties of enzymatic extract of Stichopus japonicus on murine splenocytes

Mojabanchromanol Isolated from Sargassum horneri Attenuates Particulate Matter Induced Inflammatory Responses via Suppressing TLR2/4/7-MAPK Signaling in MLE-12 Cells

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