Immunostimulatory Effects of Live Lactobacillus sakei K040706 on the CYP-Induced Immunosuppression Mouse Model

Kim, Seo-Yeon; Shin, Ji‐Sun; Chung, Kyung‐Sook; Han, Hee-Soo; Lee, Hwi-Ho; Lee, Jeonghun; Kim, Su-Yeon; Ji, Yong; Ha, Ye Jin; Kang, Jooyeon; Rhee, Young Kyoung; Lee, Kyung‐Tae

doi:10.3390/nu12113573

Cited by 18 publications

(15 citation statements)

References 46 publications

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“…The data demonstrated enrichment in Proteobacteria members and depletion of Bacteroidetes (Prevotellaceae) and Firmicutes (Lachnospiraceae and Ruminococcaceae) in the feces of cyclophosphamide-injected rats. This finding is consistent with previous reports on cyclophosphamide-induced alterations in gut microbiota (26)(27)(28)(29)(30) and may result from cyclophosphamideinduced immunodeficiency because the results of the present study indicated that transcription of the components of the intestinal immune network for immunoglobulin A (IgA) production, hematopoietic cell lineages, and platelet activation was downregulated in cyclophosphamide-injected rats. In return, dysbiosis of the gut microbiota may further impair the health of the host.…”

Section: Discussionsupporting

confidence: 94%

“…In addition to probiotics (27,28,(45)(46)(47), some other orally administered agents, such as plant extracts, active components, and their derivatives, were shown to reduce immunotoxicity of cyclophosphamide in animals (48,49). The effects of these substances are mediated by antioxidant and anti-inflammatory activities, protection of intestinal mucosal barrier, and maintenance of a balance of intestinal microbiota but are not limited to these mechanisms (48,49).…”

Section: Discussionmentioning

confidence: 99%

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Mechanism of the Immunomodulatory Effect of the Combination of Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus on Immunocompromised Rats

et al. 2021

Front. Immunol.

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Immunodeficiency is a very common condition in suboptimal health status and during the development or treatment of many diseases. Recently, probiotics have become an important means for immune regulation. The present study aimed to investigate the mechanism of the immunomodulatory effect of a combination of live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus (CBLEB), which is a drug used by approximately 10 million patients every year, on cyclophosphamide-immunosuppressed rats. Cyclophosphamide (40 mg/kg) was intraperitoneally injected to induce immunosuppression in a rat model on days 1, 2, 3, and 10. Starting from day 4, the rats were continuously gavaged with CBLEB solution for 15 days. The samples were collected to determine routine blood test parameters, liver and kidney functions, serum cytokine levels, gut microbiota, fecal and serum metabolomes, transcriptomes, and histopathological features. The results indicated that CBLEB treatment reduced cyclophosphamide-induced death, weight loss, and damage to the gut, liver, spleen, and lungs and eliminated a cyclophosphamide-induced increase in the mean hemoglobin content and GGT, M-CSF, and MIP-3α levels and a decrease in the red blood cell distribution width and total protein and creatinine levels in the blood. Additionally, CBLEB corrected cyclophosphamide-induced dysbiosis of the gut microbiota and eliminated all cyclophosphamide-induced alterations at the phylum level in rat feces, including the enrichment in Proteobacteria, Fusobacteriota, and Actinobacteriota and depletion of Spirochaetota and Cyanobacteria. Furthermore, CBLEB treatment alleviated cyclophosphamide-induced alterations in the whole fecal metabolome profile, including enrichment in 1-heptadecanol, succinic acid, hexadecane-1,2-diol, nonadecanoic acid, and pentadecanoic acid and depletion of benzenepropanoic acid and hexane. CBLEB treatment also alleviated cyclophosphamide-induced enrichment in serum D-lyxose and depletion of serum succinic acid, D-galactose, L-5-oxoproline, L-alanine, and malic acid. The results of transcriptome analysis indicated that the mechanism of the effect of CBLEB was related to the induction of recovery of cyclophosphamide-altered carbohydrate metabolism and signal transduction. In conclusion, the present study provides an experimental basis and comprehensive analysis of application of CBLEB for the treatment of immunodeficiency.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Mechanism of the Immunomodulatory Effect of the Combination of Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus on Immunocompromised Rats

et al. 2021

Front. Immunol.

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“…Th1 and Th2 secrete various types of cytokines to regulate immunity, leading to a variety of cellular responses [ 59 ]. IL-2 and IFN-γ are typical cytokines of Th1, while IL-4 and IL-10 are typical cytokines of Th2 [ 60 ]. IL-2 promotes the growth and differentiation of T cells, induce the differentiation of killer cells and stimulate the immune response [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

Ameliorative Effects of Peptides Derived from Oyster (Crassostrea gigas) on Immunomodulatory Function and Gut Microbiota Structure in Cyclophosphamide-Treated Mice

et al. 2021

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The intestinal flora is recognized as a significant contributor to the immune system. In this research, the protective effects of oyster peptides on immune regulation and intestinal microbiota were investigated in mice treated with cyclophosphamide. The results showed that oyster peptides restored the indexes of thymus, spleen and liver, stimulated cytokines secretion and promoted the relative mRNA levels of Th1/Th2 cytokines (IL-2, IFN-γ, IL-4 and IL-10). The mRNA levels of Occludin, Claudin-1, ZO-1, and Mucin-2 were up-regulated, and the NF-κB signaling pathway was also activated after oyster peptides administration. Furthermore, oyster peptides treatment reduced the proportion of Firmicutes/Bacteroidetes, increased the relative abundance of Alistipes, Lactobacillus, Rikenell and the content of short-chain fatty acids, and reversed the composition of intestinal microflora similar to that of normal mice. In conclusion, oyster peptides effectively ameliorated cyclophosphamide-induced intestinal damage and modified gut microbiota structure in mice, and might be utilized as a beneficial ingredient in functional foods for immune regulation.

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“…Probiotics commonly consist of Lactobacillus spp. or Bifidobacterium spp., which are both correlated strongly with lifespan [ 91 , 93 , 95 , 107 , 108 , 109 , 110 , 111 ]. Lactobacillus spp.…”

Section: Interventionsmentioning

confidence: 99%

“…Lactobacillus spp. prolongs C. elegans’ lifespan by about 10–20% [ 22 , 112 , 113 ] by altering the DAF-16/insulin-like pathway [ 112 ], increasing resilience to oxidative stress [ 110 ], stimulating the innate immune response [ 114 ], and mediating nuclear hormone receptors and PMK-1 signaling [ 115 ]. In aging rats, probiotics modulate AMPK activity and prevent telomere shortening [ 116 ]; protect aging bone and muscle [ 117 ]; and improve lipid, renal, and liver profiles, while also inhibiting the growth of pathogens Escherichia coli , Staphylococcus aureus , and Staphylococcus epidermidis [ 118 ].…”

Section: Interventionsmentioning

confidence: 99%

Young at Gut—Turning Back the Clock with the Gut Microbiome

et al. 2021

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Over the past century, we have witnessed an increase in life-expectancy due to public health measures; however, we have also seen an increase in susceptibility to chronic disease and frailty. Microbiome dysfunction may be linked to many of the conditions that increase in prevalence with age, including type 2 diabetes, cardiovascular disease, Alzheimer’s disease, and cancer, suggesting the need for further research on these connections. Moreover, because both non-modifiable (e.g., age, sex, genetics) and environmental (e.g., diet, infection) factors can influence the microbiome, there are vast opportunities for the use of interventions related to the microbiome to promote lifespan and healthspan in aging populations. To understand the mechanisms mediating many of the interventions discussed in this review, we also provide an overview of the gut microbiome’s relationships with the immune system, aging, and the brain. Importantly, we explore how inflammageing (low-grade chronic inflammation that often develops with age), systemic inflammation, and senescent cells may arise from and relate to the gut microbiome. Furthermore, we explore in detail the complex gut–brain axis and the evidence surrounding how gut dysbiosis may be implicated in several age-associated neurodegenerative diseases. We also examine current research on potential interventions for healthspan and lifespan as they relate to the changes taking place in the microbiome during aging; and we begin to explore how the reduction in senescent cells and senescence-associated secretory phenotype (SASP) interplay with the microbiome during the aging process and highlight avenues for further research in this area.

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Immunostimulatory Effects of Live Lactobacillus sakei K040706 on the CYP-Induced Immunosuppression Mouse Model

Cited by 18 publications

References 46 publications

Mechanism of the Immunomodulatory Effect of the Combination of Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus on Immunocompromised Rats

Mechanism of the Immunomodulatory Effect of the Combination of Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus on Immunocompromised Rats

Ameliorative Effects of Peptides Derived from Oyster (Crassostrea gigas) on Immunomodulatory Function and Gut Microbiota Structure in Cyclophosphamide-Treated Mice

Young at Gut—Turning Back the Clock with the Gut Microbiome

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