Immunosuppression by a subconjunctival implant releasing dexamethasone in a rabbit model of penetrating keratoplasty

Crouzet, Emmanuel; Garcin, Thibaud; Gauthier, Anne‐Sophie; Hé, Zhiguo; Perrache, Chantal; Delavenne, Xavier; Basset, Thierry; Péoc’h, Michel; Gain, Philippe; Thuret, Gilles

doi:10.1136/bjophthalmol-2017-310734

Cited by 6 publications

(3 citation statements)

References 17 publications

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“…To avoid angle closure and iris bowing likely to be involved in some failures, one or two small surgical iridectomies could be performed during lens removal, and mydriatic eye drop should be used. This xenograft model is not adapted to the analysis of long-term survival and tolerance because of the immunological rejection that appears to occur, as with PKP, after 3 or 4 weeks (Crouzet et al 2018). Nevertheless, we show that 3-4 weeks are sufficient to demonstrate that a TEEK exerts a normal deswelling function, which is a crucial step in the preclinical validation.…”

Section: Discussionmentioning

confidence: 78%

“…This xenograft model is not adapted to the analysis of long‐term survival and tolerance because of the immunological rejection that appears to occur, as with PKP, after 3 or 4 weeks (Crouzet et al 2018). Nevertheless, we show that 3–4 weeks are sufficient to demonstrate that a TEEK exerts a normal deswelling function, which is a crucial step in the preclinical validation.…”

Section: Discussionmentioning

confidence: 99%

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Tissue engineered endothelial keratoplasty in rabbit: tips and tricks

Crouzet

Hé

Moussa

et al. 2021

Acta Ophthalmologica

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Purpose: To report a detailed surgical procedure of tissue engineered endothelial keratoplasty (TEEK) in a rabbit model and its postoperative evaluation. Methods: TEEKs were prepared 7 days before transplantation by seeding human or rabbit corneal endothelial cells on either femtosecond laser-cut ultrathin human stromal lamellae (fs-UTSL) or femtosecond laser-cut human anterior lens capsule (fs-HALC). Thirty transplantations were performed on aphakic eyes. Recombinant tissue plasminogen activator (rTPA) was used throughout the surgery. The native endothelium was removed by full-surface scraping and central descemetorhexis. The transplantation was performed as a human Descemet's membrane endothelial keratoplasty. Controls included Descemetorhexis only and transplantation of carrier alone. Postoperative follow-up was performed by slit lamp and optical coherence tomography, followed by histology. Results: Controls remained oedematous. No fibrin occurred during surgery. All but three TEEKs adhered immediately. One/6 fs-UTSL and 9/16 fs-HALC cleared perfectly (p = 0.161). All failures could be explained by at least one of the following causes intraoperative bleeding, vitreous prolapsus, early partial detachment, postoperative irido corneal synechiea/angle closure. Presumed immune rejection was observed in three rabbits only after 4 weeks. Immunostaining with anti-human CD166 allowed to perfectly differentiate human cells from rabbit cells. In successful TEEK at 3 or 4 weeks, human cells formed a normal endothelium and started migrating outside the carrier. Conclusion: Though the transplantation of a TEEK in rabbits is a complex model with many causes of failure, established procedure including use of rTPA allows reliable preclinical study. In addition, we suggest that fs-HALC might be a potential carrier for TEEK.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Tissue engineered endothelial keratoplasty in rabbit: tips and tricks

Crouzet

Hé

Moussa

et al. 2021

Acta Ophthalmologica

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“…Therefore, this drug is usually used for patients with MD who fail to receive conservative treatment ( 11 ). Dexamethasone is a synthetic corticosteroid with anti-inflammatory, anti-allergic, and immunosuppressive effects ( 12 ). Its significant regulatory effect on the expression of myelin P0 protein in the mouse cochlea has been shown by Maeda et al ( 13 ).…”

Section: Introductionmentioning

confidence: 99%