Immunosuppression of Mice Injected With Heterologous Anti-Immunoglobulin Heavy Chain Antisera

Abstract

Neonatal injection of mice with rabbit anti-µ antiserum has been shown to produce complete loss of direct and indirect plaque-forming responses to sheep erythrocytes as well as loss of serum IgM and severe depressions of all other serum immunoglobulins. Similar injection of anti-γ1γ2 or anti-γ1 antibodies effects a loss of the indirect response but induces relatively minor alterations in serum Ig levels. Delaying initiation of anti-µ treatment until young adulthood results in a somewhat diminished effect on plaque-forming responses and serum Ig levels but triggers the release of high serum levels of an aberrant µ-bearing protein. Anti-µ suppression of genetically thymusless mice indicates that at least part of the target cells for suppression are bone marrow derived. A working hypothesis for the maturation of humoral antibody-producing cell lines as it relates to these data is discussed.

PRODUCTION OF a RUNTING SYNDROME AND SELECTIVE ΓA DEFICIENCY IN MICE BY THE ADMINISTRATION OF ANTI-HEAVY CHAIN ANTISERA

“…Treatment with anti-TM-globulin preparations re-ANTI-IG-INDUCED RIYNTING AND "yA DEFICIENCY suited in a profound generalized suppression of "yG and 3'A as well as 7M synthesis similar to the effects reported by others (21,22). In addition, a severe form of wasting disease resulted from anti-yM but not anti-'yA and anti-3'G treatment, with the dose and the time of administration of antiglobulin being critical factors in the development of this syndrome.…”

“…There are now a number of experimental findings that demonstrate suppressive effects on immunological responses as the result of exposure to anti-immunoglobulin sera including, (a) anti-allotype-induced suppression (14)(15)(16), (b) anti-idiotypeinduced suppression (17,18), (c) suppression of cell-mediated hypersensitivity and transplantation reactions in vitro (19), (d) inhibition of rosette formation (20), (e) in vivo suppression of immunoglobuljn synthesis in germfree animals (21), (f) suppression of the primary plaque-forming cell (PFC) 1 response to foreign erythrocytes in vivo (22) and in vitro (23,24), and (g) inhibition of the cellular transfer of immunoglobulin synthesis (25). The cellular basis for many of these immunosuppressive effects is still very uncertain.…”

The effect of anti-mu suppression of gammaM and gammaG on the production of gammaE.

“…However, observation that the ~M to ~G to ~A sequence could be blocked by anti-mu chain antisera (13,14) prompted this study of the effect of such antisera on ~E production. We have observed, as have others (15), that children with sex-linked agammaglobulinemia can have absent ~M, ~G, and ~/A production and yet have detectable levels of serum ~E, ~E-bearing lymphocytes, and even atopic symptoms.…”

IgE antibody and resistance to infection. I. Selective suppression of the IgE antibody response in rats diminishes the resistance and the eosinophil response to Trichinella spiralis infection.

“…Selective suppression of certain allotypes has been achieved by perinatal exposure to maternal antipaternal allotype antibodies (27) or by neonatal injections of antibodies against certain allotypic specificities (28,29). The selective suppression of the IgE response by anti-E chain antibodies is shown here for the first time.…”