Immunosuppressive Activity of Semen Persicae Ethanol Extract on Specific Antibody and Cellular Response to Ovalbumin in Mice

Abstract

The immunosuppressive activity of the ethanol extract of Semen Persicae (EESP) was studied with respect to specific antibody and cellular response to ovalbumin (OVA) in mice. The effects of EESP on mice splenocyte proliferation in vitro were measured. EESP significantly suppressed concanavalin A (ConA)- and lipopolysaccharide (LPS)-stimulated splenocyte proliferation in vitro in a concentration-dependent manner. Furthermore, the effects of EESP at three dose levels on the humoral and cellular immune responses in the OVA-immunized mice were examined. ICR Mice were immunized subcutaneously with OVA on day 0 and 14. Starting on the day of immunization, the mice were administered intraperitoneally with EESP at a single dose of 0.25, 0.5, and 1.0 mg, and cyclosporin A (CsA, positive drug) at a single dose of 0.1 mg at intervals of 7 days. On day 28, mitogen- and OVA-induced splenocyte proliferation and OVA-specific antibody level in serum were measured. EESP significantly decreased ConA-, LPS-, and OVA-induced splenocyte proliferation in the OVA-immunized mice at the dose of 1.0 mg. Meanwhile, the OVA-specific serum IgG, IgG1, and IgG2b antibody levels in the OVA-immunized mice were markedly reduced by EESP in a dose-dependent manner. The results suggest that EESP could suppress the cellular and humoral immune response in mice, and deserve further research to be developed as immunosuppressant.

Single Oral Dose Toxicity Test of Persicae Semen Aqueous Extracts in Mice

“…Aqueous extracts of PS have been traditionally used as various respiratory diseases including asthma, and hyperlipidemia related arthrosclerosis in Korea as single crude extract or ingredient of Korea traditional herbal prescriptions 4) and it has been well-documented that PS has various potent pharmacological effects including blood and cardiovascular, anti-asthma anticancer, ACE inhibitory and neuro-protective anti-inflammatory activities 5,6,8,9) , but there are no detailed toxicological assessment of PS extracts has been trials even if mouse single oral dose toxicity test, except for effects on pregnant rats 4) . Favorable effects of ACE inhibitors on the Alzheimer have been suggested 8,10) , but controversially, ACE inhibitors also showed severe toxicities 11,12) and they have been used as insecticidal agent 13) .…”

“…Maximowicz (Family: Rosaceae), and has been traditionally used as respiratory diseases including asthma, and hyperlipidemia related arthrosclerosis in Korea as single crude extract or ingredient of Korea traditional herbal prescriptions 4) . Until now, various pharmacological effects of PS extracts have been experimentally studies focused on their traditional purpose like blood and cardiovascular 5) , anti-asthma 6) , anticancer 7) , acetylcholine esterase (ACE) inhibitory 8) , neuroprotective anti-inflammatory 9) activities. Especially, PS extracts potentially inhibited the ACE in rat hippocampus, and possibly ameliorating cognitions on the Alzheimer disease 8) .…”

Optimization of pine nut (Pinus koraiensis) meal protein peptides on immunocompetence in innate and adaptive immunity response aspects

“…Currently, immunosuppressive drugs have been widely used to control undesired immune responses, such as autoimmune diseases, allergies, and allograft rejection. FK506, cyclophosphamide (CTX), and prednisone are typical immunosuppressive drugs that have been used in the clinic for many years (Zhang, Qin, & Sun, 2006). In the present study, we used CTX as an immunosuppressor to build an immunosuppressor model and focused on T lymphocytes and investigated the immunocompetence effect of PNMPP on mouse T cells both in innate and in adaptive immunity response aspects.…”