Immunosuppressive properties of deoxynivalenol

Robbana-Barnat, Saida; Lafarge‐Frayssinet, C.; Cohen, Huguette; Neish, Gordon A.; Frayssinet, C

doi:10.1016/0300-483x(88)90097-2

Cited by 58 publications

(19 citation statements)

References 7 publications

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“…We suggest that different sensitivity to mycotoxins exists in both tested cell types. This finding supports the results reported by Robbana-Barnat et al (1988), who found a different sensitivity of thymic lymphocytes and splenocytes in the blastogenic response to mitogens after administration of a DON-containing diet to mice for 1 wk.…”

Section: Discussionsupporting

confidence: 93%

Effects of diets with cereal grains contaminated by graded levels of two Fusarium toxins on selected immunological and histological measurements in the spleen of gilts1,2

Tiemann

Brüssow

Jonas

et al. 2006

Journal of Animal Science

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Feeding experiments with diets containing Fusarium toxin-contaminated wheat were conducted to clarify the pathogenesis of immunological effects of Fusarium toxins to porcine spleen cells. Contaminated diets were fed to 36 Landrace prepubertal gilts for 35 d. Concentrations (as-fed basis) of the indicator toxins deoxynivalenol (DON) and zearalenone (ZON), respectively, were 210 and 4 (control--group I), 3,070 and 88 (group II), 6,100 and 235 (group III), and 9,570 and 358 microg/kg (group IV). No signs of hyperestrogenism or uterotrophic effects were observed because of dietary treatments. The feeding of mycotoxin-contaminated diets did not cause gross pathological findings in the spleens of animals. In vivo, no inhibitory effects were detected on concanavalin A-stimulation of blood lymphocytes; however, the proliferation rate of splenocytes was inhibited (P < 0.05) in pigs fed the diet with the highest DON/ZON concentration. With in vitro studies, lower proliferation rates of blood lymphocytes and splenocytes preexposed to DON were detected. Serum IgA concentrations of pigs in group II were increased (P < 0.05) compared with the baseline value before feeding the DON/ZON diet. The histopathological data indicated elevated (P < 0.05) iron staining in the red pulp of spleens in gilts from groups I to IV after 35 d of feeding. The presence of hemosiderin particles in the spleen sections was confirmed by transmission electron microscopic investigation. Together, the results provide evidence of spleen dysfunction (hemosiderosis) in the absence of clinical signs, especially in pigs fed higher concentrations (groups III and IV) of Fusarium toxin-contaminated wheat.

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Section: Discussionsupporting

confidence: 93%

Effects of diets with cereal grains contaminated by graded levels of two Fusarium toxins on selected immunological and histological measurements in the spleen of gilts1,2

Tiemann

Brüssow

Jonas

et al. 2006

Journal of Animal Science

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“…(2006), which showed that antibody synthesis and serum immunoglobulin levels, respectively, were either unaffected or only slightly reduced in pigs receiving DON‐contaminated diets. DON has been shown to profoundly affect the immune system of mice (Tryphonas et al., 1986; Robbana‐Barnat et al., 1988), but the level of exposure to DON was much higher in mice than in pigs in the above‐mentioned trials.…”

Section: Discussionmentioning

confidence: 99%

“…This phenomenon may contribute to the reduced weight gain that is associated with DON exposure in growing pigs. In addition, high doses of DON affect the immune responses of laboratory animals (Tryphonas et al., 1986; Robbana‐Barnat et al., 1988). These findings suggest that DON may affect the immune responses in pigs and may thus reduce their resistance to infectious diseases.…”

Section: Introductionmentioning

confidence: 99%

Effects of Fusarium toxins on growth, humoral immune response and internal organs in weaner pigs, and the efficacy of apple pomace as an antidote

Gutzwiller

Czeglédi

Stoll

et al. 2007

Animal Physiology Nutrition

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A feeding trial using 220 weaner pigs which comprised two experimental series was conducted to investigate the effects of diets contaminated with the Fusarium toxins deoxynivalenol (DON) and zearalenone (ZON) and to test the hypothesis that apple pomace acts as an antidote to these mycotoxins. Two diets without contaminated wheat, containing either no pomace or 8% pomace, and two diets with naturally contaminated wheat (3.2 mg DON and 0.06 mg ZON, and 2.1 mg DON and 0.25 mg ZON per kg diet in series 1 and 2 respectively), containing either no pomace or 8% pomace were fed ad libitum for 5 weeks. Mycotoxin exposure lowered feed intake (p < 0.01) and growth (p = 0.05), and tended to decrease the energy conversion ratio (p = 0.06). Although the intake of apple pomace did not increase feed intake, it increased the growth rate (p = 0.04), mainly by restoring growth in the presence of mycotoxins (p = 0.08 for the interaction mycotoxin x pomace). In the first experimental series, the animals were immunized with a parvovirus vaccine. The percentage of seroconverting animals did not differ between the treatments (p = 0.56), which indicates that DON did not affect the humoral immune response. In the second experimental series, female piglets fed the contaminated diets had heavier uteri than piglets fed the uncontaminated diets (p < 0.01), regardless of pomace supplementation. The results show that pomace may alleviate the negative effect of DON on growth but does not counteract the hormonal effects of ZON.

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“…DON was also reported to cause a significant reduction of the thymic and splenic weights and depress the stimulation of B and T cells by mitogenes [26]. IgG, IgA and IgM secretion was significantly impaired in cultured murine lymphocytes that were exposed to DON [33].…”

Section: Discussionmentioning

confidence: 99%

Plasma haptoglobin and immunoglobulins as diagnostic indicators of deoxynivalenol intoxication

et al. 2008

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This study aimed to discover potential biomarkers for dioxynivalenol (DON) intoxication. B6C3F1 male mice were orally exposed to 0.83, 2.5 and 7.5 mg/kg body weight (bw) DON for 8 days and the differential protein expressions in their blood plasma were determined by SELDI - Time-of-Flight/Mass Spectrometry (TOF/MS) and the immunoglobulins (Igs) G, A, M and E in the serum were investigated. 11.7 kDa protein was significantly highly expressed according to DON administration and this protein was purified by employing a methyl ceramic HyperD F column with using optimization buffer for adsorption and desorption. The purified protein was identified as a haptoglobin precursor by peptide mapping with using LC/Q-TOF/MS and MALDI-TOF/MS and this was confirmed by western blotting and ELISA. IgG and IgM in serum were decreased in a dose-dependent manner and IgA was decreased at 7.5 mg/kg bw DON administration, but the IgE level was not changed. To compare the expressions of haptoglobin and the Igs patterns between aflatoxin B1 (AFB1), zearalenone (ZEA) and DON intoxications, rats were orally administered with AFB1 1.0, ZEA 240 and DON 7.5 mg/kg bw for 8 days. Haptoglobin was increased only at DON 7.5 mg/kg bw, while it was slightly decreased at ZEA 240 mg/kg bw and it was not detected at all at AFB1 1.0 mg/kg bw. IgG and IgA were decreased by DON, but IgG, IgA, IgM and IgE were all increased by AFB1. No changes were observed by ZEA administration. These results show that plasma haptoglobin could be a diagnostic biomarker for DON intoxication when this is combined with examining the serum Igs.

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Immunosuppressive properties of deoxynivalenol

Cited by 58 publications

References 7 publications

Effects of diets with cereal grains contaminated by graded levels of two Fusarium toxins on selected immunological and histological measurements in the spleen of gilts1,2

Effects of diets with cereal grains contaminated by graded levels of two Fusarium toxins on selected immunological and histological measurements in the spleen of gilts1,2

Effects of Fusarium toxins on growth, humoral immune response and internal organs in weaner pigs, and the efficacy of apple pomace as an antidote

Plasma haptoglobin and immunoglobulins as diagnostic indicators of deoxynivalenol intoxication

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