Immunosympathectomy for Preservation of Erectile Function Following Cavernous Nerve Injury

Weyne, Emmanuel; Bivalacqua, Trinity J.; Albersen, Maarten

doi:10.1016/j.eururo.2014.10.045

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…It has been suggested that ablation of a specific neuronal subpopulation by "immunosympathectomy" could be therapeutic in specific neurologic conditions, such as CNI after RP. 16,17 As our results suggest, we hypothesize that administration of galanin would preferentially stimulate parasympathetic neurons in such way that could counterbalance sympathetic reinnervation after RP and restore EF. Horizontal bar below each trace represents duration of stimulation.…”

Section: Discussionmentioning

confidence: 75%

Galanin Administration Partially Restores Erectile Function After Cavernous Nerve Injury and Mediates Endogenous Nitrergic Nerve Outgrowth In Vitro

Weyne

Hannan

Gevaert

et al. 2018

The Journal of Sexual Medicine

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We identified galanin as a potential endogenous mechanism for nerve regeneration after BCNI, which could play a physiologic role in EF recovery after radical prostatectomy. In vivo treatment with exogenous galanin was beneficial in enhancing EF recovery after BCNI, but further research is necessary to understand the underlying mechanisms. Weyne E, Hannan JL, Gevaert T, et al. Galanin Administration Partially Restores Erectile Function After Cavernous Nerve Injury and Mediates Endogenous Nitrergic Nerve Outgrowth In Vitro. J Sex Med 2018;15:480-491.

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Section: Discussionmentioning

confidence: 75%

Galanin Administration Partially Restores Erectile Function After Cavernous Nerve Injury and Mediates Endogenous Nitrergic Nerve Outgrowth In Vitro

Weyne

Hannan

Gevaert

et al. 2018

The Journal of Sexual Medicine

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“…Several investigations have been undertaken to clarify the occurrence of ED post‐nerve‐sparing RP (NS‐RP), especially concerning the hypothesis of ‘neuropraxia’. The denervated cavernosum subsequently develops apoptosis and fibrosis, resulting in poor responses to the newly regenerative peripheral nerves in later periods (Weyne et al ., ). Corporal veno‐occlusive dysfunction (CVOD) has also been proposed to develop progressively after surgery, leading to venous leak and subsequent ED (Kovanecz et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Maintenance of the contractile phenotype in corpus cavernosum smooth muscle cells by Myocardin gene therapy ameliorates erectile dysfunction in bilateral cavernous nerve injury rats

et al. 2017

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The pathophysiology of erectile dysfunction post radical prostatectomy is not clearly clarified, and the low efficacy of traditional PDE5i treatment remains a major complaint in contemporary practice. This study aimed to demonstrate phenotypic modulation in bilateral cavernous nerve injury (BCNI) rats within 7 days, and subsequently validate gene therapy with Myocardin (Mycod) by maintaining a contractile phenotype in corpus cavernosum smooth muscle cells. Initially, 36 male rats were randomly divided into BCNI and negative control (NC) groups for histological and phenotypic molecular measurements at 3, 5, and 7 days. Afterwards, an additional 30 rats received a single intra-cavernous injection of 50 μL PBS, Ad-Myocd (1 × 10 pfu/ml) or Ad-vector for 10 animals each, namely the NC+PBS, BCNI+Ad-Myocd, and BCNI+Ad-vector groups. Finally, the validity and mechanism of Myocd transfection was explored at 21 days in vivo and 48 h in vitro. Western blotting showed canonical declines in Myocd, α-SMA, and Calponin expression, as well as elevated Osteopontin (OPN) expression, before corporeal morphological and SM-to-collagen ratio changes at day 5 after injury. Overexpression of Myocd maintained the contractile phenotype of corpus cavernosum smooth muscle cells, ameliorated bilateral cavernous nerve injury rat erectile dysfunction, as well as promoted cell contractility and suppressed proliferative capacity. Simultaneously, confocal imaging revealed up-regulation and co-localization of serum response factor in gene-transferred cells. In conclusion, our study is the first to investigate corpus cavernosum smooth muscle cells phenotypes in the early stages of cavernous injury model rats, and Myocd reversed phenotypic modulation by activating serum response factor. The experimental results demonstrated the validity of gene therapy for erectile dysfunction.

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“…While this study has heavily contributed to the recent use of monoclonal antibodies in modulating endogenous neuroregeneration, it is not free from limitations. As already pointed out by Weyne and coauthors [10], it is not clear whether the reduction in penile fibrosis is due to improved oxygenation of these tissues via activity of regenerated NANC fibers or through a direct inhibitory effect on fibrotic pathways under sympathetic control, such as RhoA/ROCK signaling. Furthermore, owing to the short duration of evaluation (6 weeks), it is unclear whether this kind of treatment promotes only a more rapid EF recovery rather than a real benefit in terms of EF recovery rate.…”

mentioning

confidence: 98%

Survey of the Literature for September 2015 Issue ofSexual MedicineJournal

Pastuszak¹,

Castiglione²,

Cohen³

et al. 2015

Sexual Medicine

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Immunosympathectomy for Preservation of Erectile Function Following Cavernous Nerve Injury

Cited by 3 publications

References 14 publications

Galanin Administration Partially Restores Erectile Function After Cavernous Nerve Injury and Mediates Endogenous Nitrergic Nerve Outgrowth In Vitro

Galanin Administration Partially Restores Erectile Function After Cavernous Nerve Injury and Mediates Endogenous Nitrergic Nerve Outgrowth In Vitro

Maintenance of the contractile phenotype in corpus cavernosum smooth muscle cells by Myocardin gene therapy ameliorates erectile dysfunction in bilateral cavernous nerve injury rats

Survey of the Literature for September 2015 Issue ofSexual MedicineJournal

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