Immunotherapy using lipopolysaccharide-stimulated bone marrow-derived dendritic cells to treat experimental autoimmune encephalomyelitis

Zhou, Fang; Ćirić, Bogoljub; Zhang, G.-X.; Rostami, A.

doi:10.1111/cei.12440

Cited by 38 publications

(41 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…and the second PT injection was conducted after 48 hrs. EAE was assessed by standard clinical scores: 0.5: paralysis of half the tail, 1: paralysis of whole tail, 2: paralysis of tail and one leg, 3: paralysis of tail and two legs, 4: moribund, 5: death [21, 22, 24]. …”

Section: Methodsmentioning

confidence: 99%

“…Assays were conducted according to the manufacturer's instructions. Plates were read out in Labsystems Multiskan MCC/340 (Fisher Scientific, Suwanee, GA, USA) and data were analyzed using DSJV ELISA software (Fisher Scientific) [21-24]. …”

Section: Methodsmentioning

confidence: 99%

“…Error bars shown in this paper represent the mean and standard deviation (SD). Results were regarded as showing a significant difference if the P value was less than 0.05 [21-24]. …”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Apoptotic cell-treated dendritic cells induce immune tolerance by specifically inhibiting development of CD4+ effector memory T cells

2015

View full text Add to dashboard Cite

CD4+ memory T cells play an important role in induction of autoimmunity and chronic inflammatory responses; however, regulatory mechanisms of CD4+ memory T cell-mediated inflammatory responses are poorly understood. Here we show that apoptotic cell-treated dendritic cells inhibit development and differentiation of CD4+ effector memory T cells in vitro and in vivo. Simultaneously, i.v. transfer of apoptotic T cell-induced tolerogenic dendritic cells can block development of experimental autoimmune encephalomyelitis (EAE), an inflammatory disease of the central nervous system in C57 BL/6J mouse. Our results imply that it is effector memory CD4+ T cells, not central memory CD4+ T cells, which play a major role in chronic inflammatory responses in mice with EAE. I.V. transfer of tolerogenic dendritic cells induced by apoptotic T cells leads to immune tolerance by specifically blocking development of CD4+ effector memory T cells compared with results of EAE control mice. These results reveal a new mechanism of apoptotic cell-treated dendritic cell-mediated immune tolerance in vivo

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Apoptotic cell-treated dendritic cells induce immune tolerance by specifically inhibiting development of CD4+ effector memory T cells

2015

View full text Add to dashboard Cite

show abstract

“…22 Briefly, bone marrow was collected from the tibias and femurs. Red blood cells were depleted using 0.83 M NH 4 Cl buffer (Sigma-Aldrich Co.).…”

Section: Generation Of Dcs From Mouse Bone Marrowmentioning

confidence: 99%

Toll-like receptor 3-induced immune response by poly(D,L-lactide-co-glycolide) nanoparticles for dendritic cell-based cancer immunotherapy

Han¹,

Byeon²,

Kang³

et al. 2016

IJN

View full text Add to dashboard Cite

“…Interestingly, there are considerable experimental data showing that pre‐treatment of DC with LPS generates cells that promote tolerance rather than immunity15, 26, 27, 28, 29, 30, 31, 32 although the mechanism of tolerance induction in DC is not fully understood. Lipopolysaccharide‐activated macrophages and DC become refractory to further stimulation with LPS,33 a phenomenon known as endotoxin tolerance (ET), which has been attributed to various factors including: (i) the blockade of intracellular signalling events and subsequent gene re‐programming; (ii) up‐regulation of anti‐inflammatory cytokines like interleukin‐10 (IL‐10) and transforming growth factor‐ β (TGF‐ β ); and (iii) down‐regulation of surface expression of the TLR4 receptor 34.…”

Section: Introductionmentioning

confidence: 99%

Lipopolysaccharide‐primed heterotolerant dendritic cells suppress experimental autoimmune uveoretinitis by multiple mechanisms

et al. 2016

View full text Add to dashboard Cite

SummaryExposure of bone‐marrow‐derived dendritic cells (BMDC) to high‐dose ultrapure lipopolysaccharide for 24 hr (LPS‐primed BMDC) enhances their potency in preventing inter‐photoreceptor retinoid binding protein: complete Freund's adjuvant‐induced experimental autoimmune uveoretinitis (EAU). LPS‐primed BMDC are refractory to further exposure to LPS (= endotoxin tolerance), evidenced here by decreased phosphorylation of TANK‐binding kinase 1, interferon regulatory factor 3 (IRF3), c‐Jun N‐terminal kinase and p38 mitogen‐activated protein kinase as well as impaired nuclear translocation of nuclear factor κB (NF‐κB) and IRF3, resulting in reduced tumour necrosis factor‐α (TNF‐α), interleukin‐6 (IL‐6), IL‐12 and interferon‐β secretion. LPS‐primed BMDC also show reduced surface expression of Toll‐like receptor‐4 and up‐regulation of CD14, followed by increased apoptosis, mediated via nuclear factor of activated T cells (NFATc)‐2 signalling. LPS‐primed BMDC are not only homotolerant to LPS but are heterotolerant to alternative pathogen‐associated molecular pattern ligands, such as mycobacterial protein extract (Mycobacterium tuberculosis). Specifically, while M. tuberculosis protein extract induces secretion of IL‐1β, TNF‐α and IL‐6 in unprimed BMDC, LPS‐primed BMDC fail to secrete these cytokines in response to M. tuberculosis. We propose that LPS priming of BMDC, by exposure to high doses of LPS for 24 hr, stabilizes their tolerogenicity rather than promoting immunogenicity, and does so by multiple mechanisms, namely (i) generation of tolerogenic apoptotic BMDC through CD14:NFATc signalling; (ii) reduction of NF‐κB and IRF3 signalling and downstream pro‐inflammatory cytokine production; and (iii) blockade of inflammasome activation.

show abstract

Immunotherapy using lipopolysaccharide-stimulated bone marrow-derived dendritic cells to treat experimental autoimmune encephalomyelitis

Cited by 38 publications

References 66 publications

Apoptotic cell-treated dendritic cells induce immune tolerance by specifically inhibiting development of CD4+ effector memory T cells

Apoptotic cell-treated dendritic cells induce immune tolerance by specifically inhibiting development of CD4+ effector memory T cells

Toll-like receptor 3-induced immune response by poly(D,L-lactide-co-glycolide) nanoparticles for dendritic cell-based cancer immunotherapy

Lipopolysaccharide‐primed heterotolerant dendritic cells suppress experimental autoimmune uveoretinitis by multiple mechanisms

Contact Info

Product

Resources

About