Abstract:The international dimensions of contemporary science have significantly impacted production and use patterns of scientific knowledge, which, in turn, requires new insights of librarians, publishers and academic institutions. Despite the recognized importance of internationality in science, studies on the internationalization of scientific output are still limited and dedicated exclusively to analyzing of its diffusion and international collaboration. This study analyzes the national/international character of articles, international collaboration, references and citations of Brazilian scientific output in Evolutionary Biology in order to understand the contribution to the internationalization of science in Brazil. Analyses are based on data from the Science Citation Index of Web of Science and include 1450 articles, 60,454 references and 18,059 citing documents. Results reveal similar internationality patterns, with 99.6% of articles published in foreign journals, 90.5% international references, and 88.5% international citations. Despite recording the lowest value among the indicators (51.9%), international collaboration surpasses the national and international average and is an important characteristic in the field in Brazil, contributing to increasing the number of references and the impact of articles. Evolutionary Biology is considered a predominantly international field, whose internationality patterns increase the audience for the studies and provide greater visibility for Brazilian science.