Impact-induced cartilage damage assessed using polarisation-sensitive optical coherence tomography

Goodwin, Matthew; Workman, Joshua; Thambyah, Ashvin; Vanholsbeeck, Frédérique

doi:10.1016/j.jmbbm.2021.104326

Cited by 5 publications

(3 citation statements)

References 69 publications

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“…More recently, polarization-sensitive OCT devices have been used more frequently as they provide more detailed assessment of tissues. 22 It should be noted that previous studies evaluating OCT in the horse in joints with thinner articular cartilage such as the metacarpophalangeal 14 and intercarpal joint 23 have found that OCT was useful for examining the entire cartilage layer to the level of the subchondral bone. As previously mentioned, few studies have investigated the utility of OCT for evaluating cartilage repair tissue.…”

Section: Discussionmentioning

confidence: 99%

Correlation of Arthroscopic Grading and Optical Coherence Tomography as Markers of Early Repair and Predictors of Later Healing Evident on MRI and Histomorphometric Assessment of Cartilage Defects Implanted with Chondrocytes Overexpressing IGF-I

et al. 2023

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Objective Injury of articular cartilage is common, and due to the poor intrinsic capabilities of chondrocytes, it can precipitate joint degradation and osteoarthritis (OA). Implantation of autologous chondrocytes into cartilaginous defects has been used to bolster repair. Accurate assessment of the quality of repair tissue remains challenging. This study aimed to investigate the utility of noninvasive imaging modalities, including arthroscopic grading and optical coherence tomography (OCT) for assessment of early cartilage repair (8 weeks), and MRI to determine long-term healing (8 months). Design Large (15 mm diameter), full-thickness chondral defects were created on both lateral trochlear ridges of the femur in 24 horses. Defects were implanted with autologous chondrocytes transduced with rAAV5-IGF-I, autologous chondrocytes transduced with rAAV5-GFP, naïve autologous chondrocytes, or autologous fibrin. Healing was evaluated at 8 weeks post-implantation using arthroscopy and OCT, and at 8 months post-implantation using MRI, gross pathology, and histopathology. Results OCT and arthroscopic scoring of short-term repair tissue were significantly correlated. Arthroscopy was also correlated with later gross pathology and histopathology of repair tissue at 8 months post-implantation, while OCT was not correlated. MRI was not correlated with any other assessment variable. Conclusions This study indicated that arthroscopic inspection and manual probing to develop an early repair score may be a better predictor of long-term cartilage repair quality following autologous chondrocyte implantation. Furthermore, qualitative MRI may not provide additional discriminatory information when assessing mature repair tissue, at least in this equine model of cartilage repair.

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Section: Discussionmentioning

confidence: 99%

Correlation of Arthroscopic Grading and Optical Coherence Tomography as Markers of Early Repair and Predictors of Later Healing Evident on MRI and Histomorphometric Assessment of Cartilage Defects Implanted with Chondrocytes Overexpressing IGF-I

et al. 2023

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“…MRI, which does not use radiation, is more expensive than X-rays but can provide better images of cartilage and other structures to detect early abnormalities in OA [9]. Another imaging technique is optical coherence tomography (OCT) imaging, which has the ability to generate cross-sectional images of articular cartilage and can provide quantitative information about the condition of articular cartilage, particularly for OA caused by changes in collagen structure [10]. Although these different medical tests are more sensitive than plain radiography, they cannot be routinely applied to many patients due to its cost, and if so, they are often time-consuming and even destructive.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Degraded Cartilage Using Confocal Raman Microscopy

Jean¹,

Salehi²,

Maumus³

et al. 2023

Cartilage Disorders - Recent Findings and Treatment

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Osteoarthritis is a degenerative disease with pathological changes at the molecular level. Moreover, the damage to articular cartilage is irreversible. Early detection and the ability to follow the progression of osteoarthritis are essential to anticipate management. To characterize degraded human articular cartilage and to identify cellular changes that are precursors of phenotypic matrix changes in osteoarthritis, normal and degraded articular cartilage explants were harvested from the same patient’s knee after informed consent. The blocks were washed several times (four times) with phosphate-buffered saline (often abbreviated to PBS) and then fixed on CaF2 slides using Cell-Tak® (an adhesive glue), and the whole set was placed in different Petri dishes containing PBS for Raman measurements. The analysis of the spectroscopic data allowed to differentiate degraded cartilage from normal cartilage by applying intensity ratios of some Raman bands and/or spectral regions. In addition, peaks at 864, 929, 945, 1107, 1386, and 2887 cm−1 were identified as characteristic Raman markers of degraded cartilage. The use of confocal Raman microscopy (CRM) has proven to be relevant in providing biochemical information necessary to characterize OA cartilage. CRM appears to be a powerful tool for the diagnosis and therapeutic evaluation of osteoarthritis in both early and late stages.

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“…For example, walking, jumping, and running repeatedly exert dynamic compressions on articular cartilages, and incidental collisions such as tumbles impose strikes on tissues. Investigating the impact responses of hydrogels promises broad applications, ranging from predicting the penetration pressure of skin by a hypodermic needle for injection administration, , to the assessment of tissue damage caused by incidents, , or chronic disease like the progression of osteoarthritis under consecutive impacts . Albeit the impact responses of biological tissues have been intensively evaluated, − the examinations of the behaviors of synthetic hydrogels subjected to impact are relatively scarce. − More importantly, the synthetic hydrogels impacted so far are mostly fragile that the hydrogel may shatter into fragments after impact, which is adverse for postanalysis.…”

Section: Introductionmentioning

confidence: 99%

Strong Interfaces Enable Efficient Load Transfer for Strong, Tough, and Impact-Resistant Hydrogel Composites

Xue

Zhang

et al. 2022

ACS Appl. Mater. Interfaces

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Many biological hydrogels are mechanically robust to bear quasi-static and impact loads. In contrast, the mechanical properties of synthetic hydrogels against impact loads remain substantially unexplored, albeit their mechanical robustness under quasi-static loads has been extensively developed. Here, we report on the design and synthesis of strong, tough, and impact-resistant hydrogel composites by reinforcing Caalginate/polyacrylamide hydrogels with glass fabrics and conferring strong interfaces between the hydrogel matrix and the fibers. The fabric enables high elastic modulus, the hydrogel matrix enables large dissipation, and the strong interfaces enable efficient load transfer for synergistic strengthening and toughening, which is manifested by digital image correlation analyses. Under quasi-static loads, the hydrogel composite exhibits an elastic modulus of 35 MPa and a toughness of 206.7 kJ/m 2 . Under impact loads, a piece of 7.7 g sample bears the impact of energy of 7.4 J and resists more than 100 cycles of consecutive impact of 600 mJ. As a proof-of-concept, a hydrogel composite as a safeguard to protect fragile glasses from impact is demonstrated. Because impact phenomena are universal, it is expected that the study on the impact of hydrogels will draw increasing attention.

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Impact-induced cartilage damage assessed using polarisation-sensitive optical coherence tomography

Cited by 5 publications

References 69 publications

Correlation of Arthroscopic Grading and Optical Coherence Tomography as Markers of Early Repair and Predictors of Later Healing Evident on MRI and Histomorphometric Assessment of Cartilage Defects Implanted with Chondrocytes Overexpressing IGF-I

Correlation of Arthroscopic Grading and Optical Coherence Tomography as Markers of Early Repair and Predictors of Later Healing Evident on MRI and Histomorphometric Assessment of Cartilage Defects Implanted with Chondrocytes Overexpressing IGF-I

Characterization of Degraded Cartilage Using Confocal Raman Microscopy

Strong Interfaces Enable Efficient Load Transfer for Strong, Tough, and Impact-Resistant Hydrogel Composites

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