Monoculture and improper management may reduce soil fertility and deteriorate soil structure in Black soils (Mollisols) of Northeast China. The experiment was carried out from 2015 to 2016 in Black Soils comprising five cropping systems: continuous corn (CC), soybean-corn rotation (SC), cornsoybean rotation (CS), fallow-corn (FC), and fallow-soybean (FS). Our results showed that CS and FS treatments significantly increased mean weight diameter (MWD) and fractal dimension (D) in mechanical stability aggregates (MSAs), and increased MWD and geometric mean diameter (GMD) in water-stable aggregates (WSAs) compared with CC treatment. These two treatments were also significantly increased water-stable aggregates stability rate (WSAR), but decreased percentage of aggregates destruction (PAD) than CC treatment. Meanwhile, CS and FS treatments exhibited a higher carbon accumulation than cc treatment in bulk soils. Soil organic carbon (Soc) concentration in WSA 0.106-0.25 ,WSA 2-5 mm and WSA 0.5-1 mm had a dominant effect on aggregate stability. Simutaneously, Soc in WSA >5 mm affected SOC concentration in bulk soils. As a whole, the CS and FS treatments can increase the percentage of macro-aggregates, enhance aggregate stability, as well as increase SOC concentration in bulk soils and all soil aggregate sizes. Soil organic carbon (SOC) plays a key role in forming and stabilizing soil structure, enhancing soil physical properties, and nutrient recycling 1-3. Soil aggregate, the basic unit of soil structure, mediates many physical and chemical processes in soils 4-8 , such as soil compaction, soil nutrient recycling, soil erosion, root penetration, and crop yield 9. Aggregate stability is frequently used as an indicator of soil structure 10-12 because better soil structure and higher aggregate stability are vital to improve soil fertility, soil sustainability, and productivity 13,14. SOC influenced aggregate stability and soil structure 15,16. The stability of organic carbon in different size aggregates is different. Organic carbon in the micro-aggregates is less susceptible to change than it is in the macro-aggregates 17. The soil organic matters of different cropping systems differed based on the quantity and quality of the crop residue coverage and the environment, affecting the organic carbon contents of the soil and the aggregate stability 18. The cropping systems mainly create conditions for the decomposition and transformation of soil organic matter by changing the distribution of soil organic carbon and the active habitat of microorganisms, thereby causing changes in soil aggregates 19. Soil mean weight diameter (MWD), geometric mean diameter (GMD), fractal dimension (D), percentage of aggregates destruction (PAD) and water-stable aggregates stability rate (WSAR) are all indicators of soil aggregate stability. The larger the MWD and GMD values are, the higher the average particle size agglomeration of soil aggregates are, and the stronger the stability of soil structure is 20. Castrignano and Stelluti 21 found that ...