Impact of a mild scrotal heating on sperm chromosomal abnormality, acrosin activity and seminal alpha-glucosidase in human fertile males

Zhang, M.-H.; Zhai, Liping; Fang, Zhenya; Li, A.-N.; Yi, Qiu; Liu, Y.-X.

doi:10.1111/and.12985

Cited by 12 publications

(24 citation statements)

References 81 publications

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“…Various studies have been conducted linking several lifestyle factors/environmental exposures to elevated levels of SDF [78,94,[189][190][191][192][193][194][195][196][197][198]. These factors include (i) Physical agents such as radiation and heat; (ii) Chemical agents such as cigarette smoke, airborne pollutants, and chemotherapeutic drugs; and (iii) Biological factors including sexually transmitted infections, increasing male age, elevated body mass index (BMI) and medical conditions such as insulin dependent diabetes [78,94,[189][190][191][192][193][194][195][196][197][199][200][201].…”

Section: High Risk Patientsmentioning

confidence: 99%

Sperm DNA Fragmentation: A New Guideline for Clinicians

Agarwal

Majzoub

Baskaran

et al. 2020

World J Mens Health

185

144

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Sperm DNA integrity is crucial for fertilization and development of healthy offspring. The spermatozoon undergoes extensive molecular remodeling of its nucleus during later phases of spermatogenesis, which imparts compaction and protects the genetic content. Testicular (defective maturation and abortive apoptosis) and post-testicular (oxidative stress) mechanisms are implicated in the etiology of sperm DNA fragmentation (SDF), which affects both natural and assisted reproduction. Several clinical and environmental factors are known to negatively impact sperm DNA integrity. An increasing number of reports emphasizes the direct relationship between sperm DNA damage and male infertility. Currently, several assays are available to assess sperm DNA damage, however, routine assessment of SDF in clinical practice is not recommended by professional organizations. This article provides an overview of SDF types, origin and comparative analysis of various SDF assays while primarily focusing on the clinical indications of SDF testing. Importantly, we report four clinical cases where SDF testing had played a significant role in improving fertility outcome. In light of these clinical case reports and recent scientific evidence, this review provides expert recommendations on SDF testing and examines the advantages and drawbacks of the clinical utility of SDF testing using Strength-Weaknesses-Opportunities-Threats (SWOT) analysis.

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Section: High Risk Patientsmentioning

confidence: 99%

Sperm DNA Fragmentation: A New Guideline for Clinicians

Agarwal

Majzoub

Baskaran

et al. 2020

World J Mens Health

185

144

View full text Add to dashboard Cite

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“…[30][31][32] Before the SH, the sperm concentration was 24 to 167 × 10 6 /mL, the motility rate was 30% to 95%, and the proportion of morphologically normal sperm was 13% to 45%. Sperm concentrations, motility and normal morphology at pretreatment, treatment, and recovery phases are as shown in our previous study.…”

Section: Safety Of Sh For Subjectsmentioning

confidence: 99%

“…[30][31][32] As a continuation, this study is aimed to investigate the changes in sperm parameters, DNA, chromosome numbers, seminal biochemical substances (carnitine, EGF, and MIF), as well as reproductive hormones such as LH, FSH, and T in serum from 30 proven-fertile men subjected to SH at 43°C. [30][31][32] As a continuation, this study is aimed to investigate the changes in sperm parameters, DNA, chromosome numbers, seminal biochemical substances (carnitine, EGF, and MIF), as well as reproductive hormones such as LH, FSH, and T in serum from 30 proven-fertile men subjected to SH at 43°C.…”

Section: Introductionmentioning

confidence: 99%

Effect of scrotal heating on sperm quality, seminal biochemical substances, and reproductive hormones in human fertile men

Zhang

Zhai

Fang

et al. 2018

J of Cellular Biochemistry

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At present, male contraceptive methods are only vasectomy and condoms, so it is necessary to research on male contraceptive techniques. The aim of this study is to observe the effects of scrotal heating (SH) on semen parameters, seminal l-carnitine (LC), epidermal growth factor (EGF), macrophage migration inhibitory factor (MIF), reproductive hormones and sperm chromosome numbers of adult healthy men, and to provide the experimental data for male contraception. The scrotums of 30 healthy male volunteers were exposed to the condition of 40 to 43°C SH belt warming 40 minutes each day for successive 2 days per week. The course of SH was continuous for 3 months. Computer-assisted semen analysis and hypo-osmotic swelling test, sperm DNA integrity, l-carnitine, MIF and EGF, and sperm fluorescence in situ hybridization were performed before, during, and after SH. The serum level of follicle stimulating hormone (FSH), luteinizing hormone (LH), and testosterone (T) were measured by chemiluminescent immunoassay. The mean parameters of sperm concentration, vitality, and normal morphological sperm were significantly decreased in groups with sperms being collected during 1, 2, and 3 months of SH when compared with those in groups of pre-SH (P < 0.01). Statistically significant differences of sperm DNA fragmentation, normal sperm membrane functionality, levels of LC and MIF in semen, and LH, FSH, and T in serum were observed between the groups of before SH and after SH 3 months and the groups of during SH 1, 2, and 3 months (P < 0.001). The total rate of chromosome number for 13, 18, 21, X, and Y in the 3 months of SH was 13.7-fold greater (13.72%/1.69%) than before SH (P < 0.001). The constant SH can impact the semen quality, sperm DNA integrity, sperm chromosome, LC and MIF, and LH, FSH, and T in serum. Transient SH may be a new method for male contraception.

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“…Abnormal post-acrosomal region 25 [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31] 36 [34][35][36][37][38][39] 22 [22][23][24][25][26][27][28][29][30][31][32] 21 [20][21] 21 [21][22][23][24][25][26][27][28][29] Abnormal acrosomal region 36 72…”

Section: Discussionunclassified

“…Thin head 17 [15][16][17][18][19][20][21][22][23][24] 18 [17][18][19] 23 [18][19][20][21][22][23][24][25][26][27][28] 29 [29][30][31][32][33]* 29 [28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44] 41 [34][35][36][37][38][39]…”

Section: After the Heating Period (Phase Ii)unclassified

Mild experimental increase in testis and epididymis temperature in men: effects on sperm morphology according to spermatogenesis stages

Abdelhamid¹,

Walschaerts²,

Ahmad³

et al. 2019

Transl Androl Urol

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Background: A mild increase in testicular and epididymal temperatures in men, bulls and rams (pendulous scrotum) inhibits spermatogenesis and increases the percentage of sperm with an abnormal morphology.However, the stages of spermatogenesis that are most sensitive to a mild increase in testicular temperature in men are unknown. The aim of the present study was to explore the effects of a mild induced increase in testicular and epididymal temperature (i.e., testicular temperature maintained below the core body temperature) on sperm morphology in humans depending on the physiological time of spermatogenesis and epididymal transit.Methods: Five healthy volunteers were enrolled in an experimental study in which testicular and epididymal temperatures were increased by maintaining the testes in a supra-scrotal position with a specially designed underwear worn 15±1 h a day for 120 consecutive days. Semen collection was scheduled on specific days depending on spermatogenic stages and epididymal transit.Results: Sperm morphology and the multiple anomalies index (MAI) were analysed before, during and after heating. This mild induced increase in testicular and epididymal temperatures resulted in a significant rise in the percentage of morphologically abnormal spermatozoa on day 34 of heating, which remained elevated throughout the heating period and persisted until 45 days after cessation of heating. The MAI was significantly increased on day 20 throughout the heating period and persisted 45 days after cessation of heating. An increase in the percentage of anomalies in the sperm head, acrosome or tail occurred on days 34 and/or 45 of heating. Abnormal sperm morphology and MAI reverted to control values 73 days after cessation of heating.Conclusions: A mild sustained increase in testicular and epididymal temperature in man leads morphological abnormalities in spermatozoa mainly due to an impairment of spermiogenesis and meiosis.

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Impact of a mild scrotal heating on sperm chromosomal abnormality, acrosin activity and seminal alpha-glucosidase in human fertile males

Cited by 12 publications

References 81 publications

Sperm DNA Fragmentation: A New Guideline for Clinicians

Sperm DNA Fragmentation: A New Guideline for Clinicians

Effect of scrotal heating on sperm quality, seminal biochemical substances, and reproductive hormones in human fertile men

Mild experimental increase in testis and epididymis temperature in men: effects on sperm morphology according to spermatogenesis stages

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