Impact of acquisition and modeling parameters on test-retest reproducibility of edited GABA+

Hupfeld, Kathleen E.; Zöllner, Helge J.; Hui, Steve C.N.; Song, Yulu; Manohar, Saipavitra Murali; Yedavalli, Vivek; Oeltzschner, Georg; Prisciandaro, James J.; Edden, Richard A.E.

doi:10.1101/2023.01.20.524952

Cited by 1 publication

(1 citation statement)

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“…RF waveforms and timings can also influence the resulting signals, although, for the comparably broad MM profiles, we anticipate that these confounds will be less influential. A supplementary analysis of test–retest data acquired on a Siemens scanner 68 demonstrated improved within‐subject coefficients of variation for GABA+ estimates using our experimental MM profiles compared to the single‐Gaussian parameterization, despite the test–retest data being acquired using a different scanner vendor (Analysis S3). However, further study is required, and caution should be used when applying these experimental profiles beyond the conditions under which they were acquired.…”

Section: Discussionmentioning

confidence: 95%

GABA‐edited MEGA‐PRESS at 3 T: Does a measured macromolecule background improve linear combination modeling?

Davies‐Jenkins,

Zöllner,

Simicic

et al. 2024

Magnetic Resonance in Med

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PurposeThe J‐difference edited γ‐aminobutyric acid (GABA) signal is contaminated by other co‐edited signals—the largest of which originates from co‐edited macromolecules (MMs)—and is consequently often reported as “GABA+.” MM signals are broader and less well‐characterized than the metabolites, and are commonly approximated using a Gaussian model parameterization. Experimentally measured MM signals are a consensus‐recommended alternative to parameterized modeling; however, they are relatively under‐studied in the context of edited MRS.MethodsTo address this limitation in the literature, we have acquired GABA‐edited MEGA‐PRESS data with pre‐inversion to null metabolite signals in 13 healthy controls. An experimental MM basis function was derived from the mean across subjects. We further derived a new parameterization of the MM signals from the experimental data, using multiple Gaussians to accurately represent their observed asymmetry. The previous single‐Gaussian parameterization, mean experimental MM spectrum and new multi‐Gaussian parameterization were compared in a three‐way analysis of a public MEGA‐PRESS dataset of 61 healthy participants.ResultsBoth the experimental MMs and the multi‐Gaussian parameterization exhibited reduced fit residuals compared to the single‐Gaussian approach (p = 0.034 and p = 0.031, respectively), suggesting they better represent the underlying data than the single‐Gaussian parameterization. Furthermore, both experimentally derived models estimated larger MM fractional contribution to the GABA+ signal for the experimental MMs (58%) and multi‐Gaussian parameterization (58%), compared to the single‐Gaussian approach (50%).ConclusionsOur results indicate that single‐Gaussian parameterization of edited MM signals is insufficient and that both experimentally derived GABA+ spectra and their parameterized replicas improve the modeling of GABA+ spectra.

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Section: Discussionmentioning

confidence: 95%