Impact of aflatoxin B1 on the pharmacokinetic disposition of enrofloxacin in broiler chickens

Kalpana, Starling; Rao, G. Srinivasa; Malik, Jitendra K.

doi:10.1016/j.etap.2015.08.023

Cited by 8 publications

(4 citation statements)

References 31 publications

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“…Of note, the abovementioned fumonisin-mediated upregulation of enteric ABCB1 reported by Antonissen (2017) was linked to a decrease in the oral bioavailability of enrofloxacin [45], a known ABCB1 substrate widely used as an antimicrobial in avian species [55]. A similar decrease in enrofloxacin oral absorption was documented in broiler chickens exposed to relatively low dietary AFB1 concentrations for 42 days [56]. Scant information is available on the kinetic interactions between TP and AFB1 in chickens.…”

Section: Discussionmentioning

confidence: 68%

Turmeric Powder Counteracts Oxidative Stress and Reduces AFB1 Content in the Liver of Broilers Exposed to the EU Maximum Levels of the Mycotoxin

Amminikutty,

Spalenza,

Jarriyawattanachaikul

et al. 2023

Toxins

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The most frequent adverse effects of AFB1 in chicken are low performance, the depression of the immune system, and a reduced quality of both eggs and meat, leading to economic losses. Since oxidative stress plays a major role in AFB1 toxicity, natural products are increasingly being used as an alternative to mineral binders to tackle AFB1 toxicosis in farm animals. In this study, an in vivo trial was performed by exposing broilers for 10 days to AFB1 at dietary concentrations approaching the maximum limits set by the EU (0.02 mg/kg feed) in the presence or absence of turmeric powder (TP) (included in the feed at 400 mg/kg). The aims were to evaluate (i) the effects of AFB1 on lipid peroxidation, antioxidant parameters, histology, and the expression of drug transporters and biotransformation enzymes in the liver; (ii) the hepatic accumulation of AFB1 and its main metabolites (assessed using an in-house-validated HPLC-FLD method); (iii) the possible modulation of the above parameters elicited by TP. Broilers exposed to AFB1 alone displayed a significant increase in lipid peroxidation in the liver, which was completely reverted by the concomitant administration of TP. Although no changes in glutathione levels and antioxidant enzyme activities were detected in any treatment group, AFB1 significantly upregulated and downregulated the mRNA expression of CYP2A6 and Nrf2, respectively. TP counteracted such negative effects and increased the hepatic gene expression of selected antioxidant enzymes (i.e., CAT and SOD2) and drug transporters (i.e., ABCG2), which were further enhanced in combination with AFB1. Moreover, both AFB1 and TP increased the mRNA levels of ABCC2 and ABCG2 in the duodenum. The latter changes might be implicated in the decrease in hepatic AFB1 to undetectable levels (<LOD) in the TP supplemented group. Overall, our findings further support the use of TP as an effective feeding strategy to prevent AFB1-related adverse effects in broilers.

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Section: Discussionmentioning

confidence: 68%

Turmeric Powder Counteracts Oxidative Stress and Reduces AFB1 Content in the Liver of Broilers Exposed to the EU Maximum Levels of the Mycotoxin

Amminikutty,

Spalenza,

Jarriyawattanachaikul

et al. 2023

Toxins

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“…Pharmacokinetic parameters of enrofloxacin were modified after exposure to AFB1 (750 µg/kg) in chickens [ 58 ] However, the values of T 1/2a , T max and AUC 0–∞ of enrofloxacin were nonsignificantly increased by 17%, 26% and 17%, respectively. Also, AFB1 decreased the bioavailability and delayed the elimination of doxycycline in chickens [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

“…In this study, results revealed that AFB1 significantly increased the AUC last of TIA when broiler chickens were given either a control or AFB1 diet for 2 weeks at a rather low contamination level (20 µg/kg feed). Hence, higher AFB1 contamination levels and/or a longer exposure period may more likely result in negative effects than a two-week exposure at a low AFB1 level of 20 μg/kg in this study [ 58 , 60 ]. The IC 50 values of AFB1 and TIA were higher than 7.6 μM as reported in our in vitro results.…”

Section: Discussionmentioning

confidence: 99%

Exploration of Cytochrome P450-Related Interactions between Aflatoxin B1 and Tiamulin in Broiler Chickens

Sun,

Lootens,

Kabeta

et al. 2024

Toxins

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Poultry may face simultaneous exposure to aflatoxin B1 (AFB1) and tiamulin (TIA), given mycotoxin contamination and antibiotic use. As both mycotoxins and antibiotics can affect cytochrome P450 enzymes (CYP450), our study aimed to explore their interaction. We developed UHPLC-MS/MS methods for the first-time determination of the interaction between TIA and AFB1 in vitro and in vivo in broiler chickens. The inhibition assay showed the half maximal inhibitory concentration (IC50) values of AFB1 and TIA in chicken liver microsomes are more than 7.6 μM, indicating an extremely weak inhibitory effect on hepatic enzymes. Nevertheless, the oral TIA pharmacokinetic results indicated that AFB1 significantly increased the area under the plasma concentration-time curve (AUClast) of TIA by 167% (p < 0.01). Additionally, the oral AFB1 pharmacokinetics revealed that TIA increased the AUClast and mean residence time (MRT) of AFB1 by 194% (p < 0.01) and 136%, respectively. These results suggested that the observed inhibition may be influenced by other factors, such as transport. Therefore, it is meaningful to further explore transport and other enzymes, involved in the interaction between AFB1 and TIA. Furthermore, additional clinical studies are necessary to thoroughly assess the safety of co-exposure with mycotoxins and antibiotics.

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“…Fluoroquinolone exhibits concentration-dependent action, i.e., depending on the concentration of the drug, the duration, and intensity of bacterial lysis takes place. (Kalpana et al, 2015) [24] . Quinolones are very stable during thermal procedures, with almost no results obtained even by heating to ultra-high temperatures (shaltout et al, 2019) [44] .…”

Section: Enrofloxacinmentioning

confidence: 98%

Impact of cooking methods of meat on antibiotic residues: Review

Gowtham¹,

Muthukumar²,

B³

et al. 2020

Pharma Innovation

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Impact of aflatoxin B1 on the pharmacokinetic disposition of enrofloxacin in broiler chickens

Cited by 8 publications

References 31 publications

Turmeric Powder Counteracts Oxidative Stress and Reduces AFB1 Content in the Liver of Broilers Exposed to the EU Maximum Levels of the Mycotoxin

Turmeric Powder Counteracts Oxidative Stress and Reduces AFB1 Content in the Liver of Broilers Exposed to the EU Maximum Levels of the Mycotoxin

Exploration of Cytochrome P450-Related Interactions between Aflatoxin B1 and Tiamulin in Broiler Chickens

Impact of cooking methods of meat on antibiotic residues: Review

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