Impact of Air Pollution to Genome of Newborns

Abstract

SUMMARYThe Northern Moravia Region is the most polluted region in the Czech Republic by particulate matter (PM 2.5 ) and carcinogenic polycyclic aromatic hydrocarbons (c-PAHs) as benzo [a]pyrene (B[a]P) by heavy industry and local heating. This specific situation was used to study the impact of air pollution on newborns in the exposed Karviná district and control district of České Budějovice. Biological material from newborns and mothers was collected in summer and winter seasons. This project is highly detailed, analyzing the concentrations of PAHs in ambient air and diet, in human breast milk, in the urine of mothers and newborns, using biomarkers of genetic damage as DNA adducts and gene expression analysis, biomarkers of oxidative stress as 8-oxodG adducts and lipid peroxidation (15-F2t-isoprostane immunoassay). All 400 children, for whom the biomarker data at delivery were obtained, will be followed for morbidity up to 2 years of age. The Northern Moravia Region seems to be to be a model area for studying the long-term impact of human health exposure to c-PAHs. Our observations will indicate possible genetic and oxidative damage in newborns, which may significantly affect their morbidity.

Impact of Air Pollution on the Health of the Population in Parts of the Czech Republic

“…It significantly increased in newborns in Karvina. DNA adducts [52], oxidative damage (DNA oxidation, lipid peroxidation) [53], and whole genome expression revealed a deregulation of processes associated with immune response or oxidative stress response [54]. In the urine of newborns, monohydroxylated metabolites of PAHs (OH-PAHs) were analyzed.…”

Morbidity of children under 2 years of age in the districts with different degrees of air pollution

“…do studie byly zařazeny děti matek nekuřaček, s donošeným těhotenstvím. (3) Na tuto studii jsme navázali, požádali jsme o spolupráci pediatry, u nichž byly děti registrovány, a jejich prostřednictvím matky dětí. pokud matky souhlasily, podepsaly informovaný souhlas (schválený etickou komisí Ústavu experimentální medicíny akademie věd Čr) a vyplnily dotazník týkající se domácího prostředí dítěte.…”

Possible relationship between respiratory diseases and urinary concentrations of polycyclic aromatic hydrocarbon metabolites - a pilot study

“…Furthermore, we tried to find the cause of the differen-ces in PAH exposure by investigating the information provided in personal questionnaires. The district of České Budějovice city in Southern Bohemia was selected as a control locality, according to our previous studies (Sram et al, 2016;Veleminsky et al, 2016). In the 1970s and 1980s, the district of Most city in Northern Bohemia was characterised by significant air pollution due to power plants and local heating emissions, which used brown coal with a high content of sulphur.…”