Impact of an Anticaries Mouthrinse onIn VitroRemineralization and Microbial Control

Abstract

Objective. The objective of this research was to evaluate the caries control potential of a new fluoride mouthrinse that also contained antimicrobial agents and a biofilm disrupting agent using different in vitro models. Methods. Four in vitro studies were conducted to assess the performance of this three pronged approach to caries control: (1) traditional enamel fluoride uptake, (2) surface microhardness study using pH cycling model and subsequent fluoride uptake, (3) a salivary biofilm flow-through study to determine the anti-microbial activity, and (4) a single species biofilm model measuring effect on biofilm matrix disruption. Results. The data showed that a LISTERINE rinse with fluoride, essential oils and xylitol was superior in promoting enamel fluoride uptake and in enhancing antimicrobial activity over traditional commercially available fluoridated products. An increase of the surface microhardness was observed when the LISTERINE rinse was used in combination with fluoridated toothpaste versus the fluoridated toothpaste alone. Finally, it was demonstrated that xylitol solutions disrupted and reduced the biovolume of biofilm matrix of mature Streptococcus mutans. Conclusion. These in vitro studies demonstrated that a fluoride mouthrinse with antimicrobial agent and biofilm matrix disrupting agent provided multifaceted and enhanced anti-caries efficacy by promoting remineralization, reducing acidogenic bacteria and disrupting biofilm matrix.

Contribution of Essential Oils to the Fight against Microbial Biofilms—A Review

“…It was also demonstrated the benefit of association of these EOs with xylitol in mouthrinse against S. mutans-derived biofilms, independent of the type of treatment or age of biofilm. This is a very promising treatment for the treatment and prevention of caries [63].The Mentha piperita and Rosmarinus officinalis EOs proved to be effective against S. mutans, one of the main agents of dental caries. Of the Mentha piperita EO, having a menthol concentration below 3.6% was more effectivethan rosemary oil(containing piperitone as the main component) and chlorhexidine (at 4000 and 8000 ppm).…”

Effect of a novel commercial potassium-oxalate containing tooth-desensitizing mouthrinse on the microhardness of resin composite restorative materials with different monomer compositions

“…Water absorption of Kalore may be similar to UDMA based composite resins. Moreover, Sun et al (20) also reported that it is important that the composite resin presents uniform filler particle distribution in the polymer network to minimize the formation of filler-rich and filler-depleted areas within the composites. This is especially important regarding the performance of composites in aqueous solutions, since voids or nonbonding spaces at the filler/matrix interface may increase the water sorption of composites (5).…”

“…One high molecular weight monomer called DX-511, based on urethane dimethacrylate and present in the commercial composite Kalore (GC Co, Tokyo-Japan), leads to shrinkage reduction due the low reactive group concentration available for reaction and has been shown to lower polymerization stress and shrinkage values compared with conventional composites (18). Also a reported upgrade in the mouthrinse formulation with fluoride, essential oils and xylitol will further enhance the treatment effect (20). The investigated null hypothesis was that microhardness values of different monomer systems would be affected with the immersion in a desensitizing mouthrinse.…”

Evaluation of the effect of different commercial mouthrinses on the viability and activity of microcosm biofilm and on enamel demineralization

“…A recent review pointed out that few studies have been done to elucidate the antiplaque effect of these agents (BRAGA; PIRES; MAGALHÃES, 2017). In general, the studies have shown that the essential oil has comparable effect than chlorexidine and both are better than cetylpyridinium chloride on the cariogenic biofilm (GUGGENHEIM; OYANAGI;SUN et al, 2014;FREIRES et al, 2015;QUINTAS et al, 2015). However, most studies have been done in vitro, using monospecies or multispecies biofilm models, whose the main response variable was the antimicrobial effect and not the anticaries effect.…”