Impact of antiseptics on <i>Chlamydia trachomatis</i>
 growth

Párducz, László; Eszik, Ildikó; Wagner, Gábor; Burián, Katalin; Endrész, Valéria; Virók, Dezső

doi:10.1111/lam.12625

Cited by 12 publications

(5 citation statements)

References 24 publications

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“…3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2H‐tetrazolium bromide (MTT) assay was performed to characterize the maximum nontoxic concentration of the NPs on HeLa cells after 48 h of incubation as was described previously (Párducz et al . ).…”

Section: Methodsmentioning

confidence: 97%

Nonactivated titanium-dioxide nanoparticles promote the growth ofChlamydia trachomatisand decrease the antimicrobial activity of silver nanoparticles

et al. 2017

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Section: Methodsmentioning

confidence: 97%

Nonactivated titanium-dioxide nanoparticles promote the growth ofChlamydia trachomatisand decrease the antimicrobial activity of silver nanoparticles

et al. 2017

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“…The Chlamydiales order bacteria are eukaryotic cells obligate intracellular parasites [5]. Chlamydial infections in animals cause a wide range of clinical diseases that do not have clear signs.…”

Section: Introductionmentioning

confidence: 99%

Retrospective analysis of the spread of cat chlamydia and features of its treatment with a drug based on azithromycin

et al. 2020

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The Chlamydiales order bacteria are eukaryotic cells obligate intracellular parasites. The major purpose of the research was to study the prevalence, clinical progression and treatment of Chlamydia in cats with an azithromycin-based drug. A total of 3,388 samples for chlamydia were studied in 2019, of which 243 proved to be positive. Chlamydia was diagnosed using the polymerase chain reaction (PCR) technique. The Azithronit drug was administered 0.5 ml per animal, 1 time per day, for 7 days. Hematological parameters were determined using the IDEXX Laser Cyte hematological analyzer, while the biochemical parameters were determined using the IDEXX Catalist analyzer (USA). In Moscow, chlamydial infection plays a significant role in the cats inflammatory eye diseases etiology. The overall infection rate of the Moscow cat population totalled 7.2%. In young animals, the incidence was higher (28.3%) than in adults (19.5%). In terms of gender, the incidence of chlamydia in female cats (56%) was higher than in males (44%). The main forms of chlamydia manifestations were those of purulent and serous conjunctivitis - 69.8% and 20.5%, respectively, keratitis - 8.7%. The treatment with azithromycin-based drugs requires only a single daily administration for 7 days to ensure the clinical signs elimination.

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“…Direct PCR and direct qPCR eliminate the purification step, significantly shortening the protocol, but the inhibitory effect of the direct sample can be present. Previously, direct qPCR methods have been successfully applied to monitor Chlamydia and herpes simplex virus-2 growth and the antimicrobial effects of various compounds [6][7][8][9][10][11]. In this study, we want to leave out the DNA purification step and develop a direct qPCR detection method that is suitable to detect Mycoplasma contamination within U937 cell cultures.…”

Section: Introductionmentioning

confidence: 99%

Direct qPCR is a sensitive approach to detect Mycoplasma contamination in U937 cell cultures

et al. 2019

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Objective: We aim to directly detect Mycoplasma DNA in a U937 suspension cell culture without using DNA purification. In order to make Mycoplasma contamination monitoring easier, we optimized a commercially available quantitative PCR (qPCR)-based detection kit. We compared the sensitivity of direct qPCR against qPCR with a purified DNA template. Results: Our findings indicate that qPCR worked optimally with a 6 μl sample volume and a 52 °C annealing-extension temperature. We were able to decrease the annealing-extension step time from 60 to 20 s without any major decrease in reaction sensitivity. The total cycle time of optimized direct qPCR was 65 min. The optimized qPCR protocol was used to detect Mycoplasma DNA before and after DNA purification. Our findings indicate that direct qPCR had a higher sensitivity than regular qPCR. Ct levels produced by direct qPCR with 6 μl templates were almost identical to Ct levels produced by regular qPCR with DNA purified from a 60 μl cell culture sample (23.42 vs 23.49 average Ct levels, respectively). The optimized direct qPCR protocol was successfully applied to monitor the elimination of Mycoplasma contamination from U937 cell cultures.

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Impact of antiseptics on Chlamydia trachomatis growth

Cited by 12 publications

References 24 publications

Nonactivated titanium-dioxide nanoparticles promote the growth ofChlamydia trachomatisand decrease the antimicrobial activity of silver nanoparticles

Nonactivated titanium-dioxide nanoparticles promote the growth ofChlamydia trachomatisand decrease the antimicrobial activity of silver nanoparticles

Retrospective analysis of the spread of cat chlamydia and features of its treatment with a drug based on azithromycin

Direct qPCR is a sensitive approach to detect Mycoplasma contamination in U937 cell cultures

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