Impact of aspirin on the transcriptome of Streptococcus pneumoniae D39

Afzal, Muhammad; Shafeeq, Sulman

doi:10.1016/j.gdata.2017.02.013

Cited by 5 publications

(4 citation statements)

References 10 publications

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“…2A ; Data Set S1 in the supplemental material). Consistent with prior studies ( 33 , 42 ), we observed that this subinhibitory level of aspirin led to a largely downregulatory shift in gene expression. Using a cutoff P value of <0.05 and a 2-fold change, we observed 53 genes with significantly lower expression and only 2 genes (both encoding proteins of unknown function) with significantly higher expression when Fn7-1 was grown in 1 mM aspirin.…”

Section: Resultssupporting

confidence: 92%

“…Aspirin and salicylic acid alter Fn7-1 growth in culture. To probe whether aspirin affects the well-studied F. nucleatum strain Fn7-1, we assayed growth in media supplemented with increasing aspirin concentrations comparable to those used in prior studies (31,33,41) (Fig. 1A).…”

Section: Resultsmentioning

confidence: 99%

“…Aspirin and other NSAIDs cause shifts in the microbiota (28)(29)(30). Aspirin and salicylic acid, its primary bioactive metabolite, directly affect bacteria by inhibiting growth (31,32) and altering virulence factor expression (33)(34)(35)(36)(37). While some bacteria have specific salicylic acid-responsive regulators (38,39), how aspirin and salicylic acid drive these changes in other bacteria is less understood.…”

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confidence: 99%

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Aspirin Modulation of the Colorectal Cancer-Associated Microbe Fusobacterium nucleatum

Brennan

Nakatsu

Comeau

et al. 2021

mBio

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Aspirin is a chemopreventive agent for colorectal adenoma and cancer (CRC) that, like many drugs inclusive of chemotherapeutics, has been investigated for its effects on bacterial growth and virulence gene expression. Given the evolving recognition of the roles for bacteria in CRC, in this work, we investigate the effects of aspirin with a focus on one oncomicrobe—Fusobacterium nucleatum. We show that aspirin and its primary metabolite salicylic acid alter F. nucleatum strain Fn7-1 growth in culture and that aspirin can effectively kill both actively growing and stationary Fn7-1. We also demonstrate that, at levels that do not inhibit growth, aspirin influences Fn7-1 gene expression. To assess whether aspirin modulation of F. nucleatum may be relevant in vivo, we use the ApcMin/+ mouse intestinal tumor model in which Fn7-1 is orally inoculated daily to reveal that aspirin-supplemented chow is sufficient to inhibit F. nucleatum-potentiated colonic tumorigenesis. We expand our characterization of aspirin sensitivity across other F. nucleatum strains, including those isolated from human CRC tissues, as well as other CRC-associated microbes, enterotoxigenic Bacteroides fragilis, and colibactin-producing Escherichia coli. Finally, we determine that individuals who use aspirin daily have lower fusobacterial abundance in colon adenoma tissues, as determined by quantitative PCR performed on adenoma DNA. Together, our data support that aspirin has direct antibiotic activity against F. nucleatum strains and suggest that consideration of the potential effects of aspirin on the microbiome holds promise in optimizing risk-benefit assessments for use of aspirin in CRC prevention and management. IMPORTANCE There is an increasing understanding of the clinical correlations and potential mechanistic roles of specific members of the gut and tumoral microbiota in colorectal cancer (CRC) initiation, progression, and survival. However, we have yet to parlay this knowledge into better CRC outcomes through microbially informed diagnostic, preventive, or therapeutic approaches. Here, we demonstrate that aspirin, an established CRC chemopreventive, exhibits specific effects on the CRC-associated Fusobacterium nucleatum in culture, an animal model of intestinal tumorigenesis, and in human colonic adenoma tissues. Our work proposes a potential role for aspirin in influencing CRC-associated bacteria to prevent colorectal adenomas and cancer, beyond aspirin’s canonical anti-inflammatory role targeting host tissues. Future research, such as studies investigating the effects of aspirin on fusobacterial load in patients, will help further elucidate the prospect of using aspirin to modulate F. nucleatum in vivo for improving CRC outcomes.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Aspirin Modulation of the Colorectal Cancer-Associated Microbe Fusobacterium nucleatum

Brennan

Nakatsu

Comeau

et al. 2021

mBio

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“…Afzal et al 2017 showed that expression of several S. pneumoniae geneswas differentially expressed in presence of aspirin [25] .…”

Section: According To the Guidelines Of The Clinical Andmentioning

confidence: 99%

In Vitro Activity of AspirinAlone and in Combination with Cefixime Against Clinical Isolates of Streptococcal Pyogenes, A Pilot Study

Hassan¹,

Altimimi²,

Alfadhul³

et al. 2021

IJFMT

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Background: Streptococcus pyogenes most commonly cause pharyngitis, also has been associated with invasive infection as sepsis & fulminate systemic infections . It is imperative cause for fatal condition in children like acute rheumatic fever & rheumatic heart disease. Development of strains that resistant to antibiotics makes such disease a dangerous clinical challenge particularly in children, so in order to overcomingthis resistance, a nonsteroidal anti-inflammatory drugs (NSAID) is repurposed as antimicrobials against this microorganisms to be combined with the anti-microbial suggested by WHO rule of care.Aims: To evaluate the antimicrobial activity of aspirin & an aspirin-cefixime combination against multidrugresistant S.pyogenes isolated from clinical cases of pharyngitis.Materials, Patients and Methods: S.pyogenes,a multidrug-resistant strain, was isolated from patients had pharyngitis. It was cultivated for determination of MIC for aspirin-cefixime combination in relationship with each drug alone &to determine combination index.Results and Conclusions: a significant synergism was found between aspirin-cefixime ( combination index ˂ 1) P= 0.009& Z score= 2.5 further confirmation of MIC had assessed bytwo folds of dilutions to achieve reasonable evidence. Conclusion:There was a significant synergistic effect achieved from combining aspirin with cefixime against S.pyogenes isolate in culture . Recommendations:We recommended further evaluation for the antistreptococcal effect of the combined aspirin-cefixime on more dilutions of MIC assay & to investigate data of this combination clinically in patients with S.pyogenes pharyngitis.

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A study on the degradation efficiency of fluoranthene and the transmembrane protein mechanism of Rhodococcus sp. BAP-1 based on iTRAQ

Jiang

Wang

et al. 2020

Science of The Total Environment

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Impact of aspirin on the transcriptome of Streptococcus pneumoniae D39

Cited by 5 publications

References 10 publications

Aspirin Modulation of the Colorectal Cancer-Associated Microbe Fusobacterium nucleatum

Aspirin Modulation of the Colorectal Cancer-Associated Microbe Fusobacterium nucleatum

In Vitro Activity of AspirinAlone and in Combination with Cefixime Against Clinical Isolates of Streptococcal Pyogenes, A Pilot Study

A study on the degradation efficiency of fluoranthene and the transmembrane protein mechanism of Rhodococcus sp. BAP-1 based on iTRAQ

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