Impact of Bacterial Biofilm in Veterinary Medicine: An Overview

Chakraborty, Satyaki; Dutta, T. K.; De, Arpan; Das, Madhuchhanda; Ghosh, Subhamoy

doi:10.20546/ijcmas.2018.704.366

Cited by 16 publications

(13 citation statements)

References 59 publications

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“…Agar test. According to the national health institutes publications, microorganisms that produce biofilm are related to more than 65-80% of the bacterial infections [32][33][34][35]. Hassan et al [32] evaluated the ability of biofilm production in 110 clinical isolates of pathogenic bacteria, of different species, by the method of violet crystal staining.…”

Section: Fig1: Evaluation Of Biofilm Production By Congo Redmentioning

confidence: 99%

Antibacterial and antibiofilm activities of quercetin against clinical isolates of Staphyloccocus aureus and Staphylococcus saprophyticus with resistance profile

Júnior¹,

Santos²,

Campos³

et al. 2018

IJEAB

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The aim of this study was to determine the antibacterial and antibiofilm properties of quercetin against clinical isolates of Staphyloccocus aureus and Staphylococcus saprophyticus with resistance profile. The antibacterial activity of quercetin was performed by the determination of the minimum inhibitory concentration (MIC) through the microdilution method according to the Clinical and Laboratory Standards Institute (CLSI). The percentage of inhibition of Staphylococcus spp. biofilm, after treatment with sub-inhibitory concentrations of quercetin (MIC/2 and MIC/4), was evaluated by the violet crystal assay. Quercetin showed an antimicrobial activity against clinical isolates of methicillin-susceptible S.

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Section: Fig1: Evaluation Of Biofilm Production By Congo Redmentioning

confidence: 99%

Antibacterial and antibiofilm activities of quercetin against clinical isolates of Staphyloccocus aureus and Staphylococcus saprophyticus with resistance profile

Júnior¹,

Santos²,

Campos³

et al. 2018

IJEAB

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“…Many species of bacterial genera of human and veterinary importance such as Escherichia , Staphylococcus , Pseudomonas , Pasteurella , Bacillus , Salmonella etc. cause infections that are difficult to treat due to their ability to form biofilms [13, 14]. Biofilms could be involved in over 60% of microbial infections [15, 16] while two-thirds of all human bacterial infections are caused by the biofilms [17].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and antibiofilm activity of acetone leaf extracts of nine under-investigated south African Eugenia and Syzygium (Myrtaceae) species and their selectivity indices

Famuyide

Aro

Fasina

et al. 2019

BMC Complement Altern Med

157

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Background Antimicrobial resistance (AMR) remains an important global health issue but the gap between AMR and development of new antimicrobials is increasing. Plant extracts may have good activity per se or may be sources of effective antimicrobial compounds which can act against planktonic and/or biofilms of pathogens. We determined the antimicrobial efficacy and cytotoxicity of some under-investigated plants from the Myrtaceae family endemic to South Africa. The ability of the plant extracts to inhibit or destroy pre-formed bacterial biofilms was also determined. Methods Based on previous preliminary in vitro screening and on chemotaxonomy, nine species from the Myrtaceae family were selected. The antimicrobial activity of the crude acetone leaf extracts was determined against six common nosocomial pathogens, namely: Gram-positive bacteria ( Bacillus cereus , Enterococcus faecalis , Staphylococcus aureus ), Gram-negative bacteria ( Escherichia coli , Pseudomonas aeruginosa , Salmonella Typhimurium) using a two-fold serial microdilution assay with p-iodonitrotetrazolium violet as growth indicator. The number of antimicrobial compounds present in extracts was determined by bioautography. Cytotoxicity of extracts was determined against Vero kidney cells using a colorimetric tetrazolium-based assay. The total antibacterial activity (TAA) in ml/g and selectivity index (LC 50 /MIC) of the plant extracts were calculated. A modified crystal violet assay was used to determine the antibiofilm activity of the extracts. Results Syzygium legatii , Syzygium masukuense , and Syzygium species A had the best activities against Gram-negative and Gram-positive bacteria (MIC) values ranging from 0.04–0.08 mg/ml. Eugenia erythrophylla had the best MIC (0.02 mg/ml) against Bacillus cereus . Many extracts had relatively low cytotoxicity (LC 50 > 20 μg/ml) leading to reasonable selectivity indices. Three leaf extracts ( Syzygium masukuense , Syzygium species A, and Eugenia natalitia ) were moderately cytotoxic (20 μg/ml < LC 50 < 100 μg/ml). The plant extracts had a good capacity to reduce biofilm formation and good to poor potential to destroy pre-formed biofilms. Conclusions The plant species examined in this study had varying degrees of antibacterial activity against bacterial planktonic and biofilm forms with some having good activity against both forms. Several of these selected species may be potential candidates for further investigation to isolate antimicrobial compounds and to determ...

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“…Meanwhile, biofilms growing on organic surfaces are associated with chronic infections of epithelial and mucosal surfaces, such as Pseudomonas-associated cystic fibrosis [4], urinary tract infections, and foot ulcers [5]. In animals, these infections manifest as mastitis, wound lesions, enteritis, and pneumonia [6]. Treatment for biofilm infections is scarce as many biofilms have an inherent resistance to conventional antibiotics due to the protective EPS matrix and can persist even after treatment with concentrations a thousand times their minimal inhibitory concentration [7].…”

Section: Introductionmentioning

confidence: 99%

The Addition of a Synthetic LPS-Targeting Domain Improves Serum Stability While Maintaining Antimicrobial, Antibiofilm, and Cell Stimulating Properties of an Antimicrobial Peptide

et al. 2020

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Multi-drug resistant (MDR) bacteria and their biofilms are a concern in veterinary and human medicine. Protegrin-1 (PG-1), a potent antimicrobial peptide (AMP) with antimicrobial and immunomodulatory properties, is considered a potential alternative for conventional antibiotics. AMPs are less stable and lose activity in the presence of physiological fluids, such as serum. To improve stability of PG-1, a hybrid peptide, SynPG-1, was designed. The antimicrobial and antibiofilm properties of PG-1 and the PG-1 hybrid against MDR pathogens was analyzed, and activity after incubation with physiological fluids was compared. The effects of these peptides on the IPEC-J2 cell line was also investigated. While PG-1 maintained some activity in 25% serum for 2 h, SynPG-1 was able to retain activity in the same condition for up to 24 h, representing a 12-fold increase in stability. Both peptides had some antibiofilm activity against Escherichia coli and Salmonella typhimurium. While both peptides prevented biofilm formation of methicillin-resistant Staphylococcus aureus (MRSA), neither could destroy MRSA’s pre-formed biofilms. Both peptides maintained activity after incubation with trypsin and porcine gastric fluid, but not intestinal fluid, and stimulated IPEC-J2 cell migration. These findings suggest that SynPG-1 has much better serum stability while maintaining the same antimicrobial potency as PG-1.

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Impact of Bacterial Biofilm in Veterinary Medicine: An Overview

Cited by 16 publications

References 59 publications

Antibacterial and antibiofilm activities of quercetin against clinical isolates of Staphyloccocus aureus and Staphylococcus saprophyticus with resistance profile

Antibacterial and antibiofilm activities of quercetin against clinical isolates of Staphyloccocus aureus and Staphylococcus saprophyticus with resistance profile

Antibacterial and antibiofilm activity of acetone leaf extracts of nine under-investigated south African Eugenia and Syzygium (Myrtaceae) species and their selectivity indices

The Addition of a Synthetic LPS-Targeting Domain Improves Serum Stability While Maintaining Antimicrobial, Antibiofilm, and Cell Stimulating Properties of an Antimicrobial Peptide

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