Impact of beta-cypermethrin on soil microbial community associated with its bioavailability: A combined study by isothermal microcalorimetry and enzyme assay techniques

Zhuang, Rensheng; Chen, Huilun; Yao, Jun; Li, Zhe; Burnet, Julia Ellis; Choi, Martin M.F.

doi:10.1016/j.jhazmat.2011.02.034

Cited by 42 publications

(16 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pyrethroid insecticides have been used worldwide due to their potent toxic activity against various insect pests, and in particular, they have become the dominate insecticides in retail markets [1], [2]. Since 2000, the usage of these pesticides has been increased by as much as 25%, and their application is anticipated to be further increased due to the reduced use of organophosphate insecticides like diazinon and chlorpyrifos [3].…”

Section: Introductionmentioning

confidence: 99%

Monooxygenase, a Novel Beta-Cypermethrin Degrading Enzyme from Streptomyces sp

et al. 2013

View full text Add to dashboard Cite

The widely used insecticide beta-cypermethrin has become a public concern because of its environmental contamination and toxic effects on mammals. In this study, a novel beta-cypermethrin degrading enzyme designated as CMO was purified to apparent homogeneity from a Streptomyces sp. isolate capable of utilizing beta-cypermethrin as a growth substrate. The native enzyme showed a monomeric structure with a molecular mass of 41 kDa and pI of 5.4. The enzyme exhibited the maximal activity at pH 7.5 and 30°C. It was fairly stable in the pH range from 6.5–8.5 and at temperatures below 10°C. The enzyme activity was significantly stimulated by Fe2+, but strongly inhibited by Ag+, Al3+, and Cu2+. The enzyme catalyzed the degradation of beta-cypermethrin to form five products via hydroxylation and diaryl cleavage. A novel beta-cypermethrin detoxification pathway was proposed based on analysis of these products. The purified enzyme was identified as a monooxygenase by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry analysis (MALDI-TOF-MS) and N-terminal protein sequencing. Given that all the characterized pyrethroid-degrading enzymes are the members of hydrolase family, CMO represents the first pyrethroid-degrading monooxygenase identified from environmental microorganisms. Taken together, our findings depict a novel pyrethroid degradation mechanism and indicate that the purified enzyme may be a promising candidate for detoxification of beta-cypermethrin and environmental protection.

show abstract

Section: Introductionmentioning

confidence: 99%

Monooxygenase, a Novel Beta-Cypermethrin Degrading Enzyme from Streptomyces sp

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Its half-life ranges from 14.6 to 76.2 days in soil (Gu et al 2008), depending on the physicochemical properties of the soil as well as on the microbial activity of the soil (Fenlon et al 2011a, b). Microbial degradation is considered to be the most significant process determining the fate and behavior of β-CP in soil (Boucard et al 2008;Gu et al 2008;Fenlon et al 2011a, b;Zhuang et al 2011). In the natural environment, β-CP can be converted to 3-phenoxybenzaldehyde (3-PBA), a toxic metabolite that is refractory to microbial degradation (Xu et al 2007;Yuan et al 2010).…”

mentioning

confidence: 99%

Bioremediation of β-cypermethrin and 3-phenoxybenzaldehyde contaminated soils using Streptomyces aureus HP-S-01

Chen

Geng

Xiao

et al. 2011

Appl Microbiol Biotechnol

100

View full text Add to dashboard Cite

Using laboratory and field experiments, the ability of Streptomyces aureus HP-S-01 to eliminate β-cypermethrin (β-CP) and its metabolite 3-phenoxybenzaldehyde (3-PBA) in soils was investigated. In the laboratory, 80.5% and 73.1% of the initial dose of β-CP and 3-PBA (50 mg kg(-1)) was removed in sterilized soils within 10 days, respectively, while in the same period, disappearance rate of β-CP and 3-PBA in non-sterilized soils was higher and reached 87.8% and 79.3%, respectively. Furthermore, the disappearance process followed the first-order kinetics and the half-life (T (1/2)) for β-CP and 3-PBA reduced by 20.3-52.9 and 133.7-186.8 days, respectively, as compared to the controls. The addition of sucrose to the soils enhanced the ability of strain HP-S-01 to eliminate β-CP and 3-PBA. Similar results were observed in the field experiments. The introduced strain HP-S-01 quickly adapted to the environment and rapidly removed β-CP and 3-PBA without any lag phases in the field experiments. Compared with the controls, 47.9% and 67.0% of applied dose of β-CP and 3-PBA was removed from the soils without extra carbon sources and 52.5% and 73.3% of β-CP and 3-PBA was eliminated in soils supplemented with sucrose within 10 days, respectively. Analysis of β-CP degradation products in soil indicated that the tested strain transform β-CP to 3-PBA and α-hydroxy-3-phenoxy-benzeneacetonitrile. However, both intermediates were transient and they disappeared after 10 days. Therefore, the selected actinomyces strain HP-S-01 is suitable for the efficient and rapid bioremediation of β-CP contaminated soils.

show abstract

“…Each object within the same class is similar to the others but different from those in other classes based on a predetermined selection criterion (Zhuang et al 2011). Heml is an easy-to-use tool with transformation and visualization of multi-dimensional data in a single heat map and can provide a concise but comprehensive presentation of biothermokinetics and multiple clustering strategies for analyzing the data (Deng et al 2014).…”

Section: Chemometric Analysismentioning

confidence: 99%

Antibacterial evaluation of Salvia miltiorrhizae on Escherichia coli by microcalorimetry coupled with chemometrics

Ying

Zhang

et al. 2017

AMB Expr

View full text Add to dashboard Cite

For seeking novel antibacterial agents with high efficacy and low toxicity to deal with drug resistance, the effects of Salvia miltiorrhizae from various sources on Escherichia coli were evaluated by microcalorimetry coupled with chemometrics. Firstly, the heat-flow power-time curves of E. coli growth affected by different S. miltiorrhizae samples were recorded. Then, some crucial quantitative thermo-kinetic parameters including growth rate constant, heat-flow power and heat output, etc. were obtained from theses curves and were further investigated by some powerful chemometric techniques including similarity analysis, multivariate analysis of variance, hierarchical clustering analysis and principle component analysis. By analyzing the principle parameters, growth rate constant of the second exponential phase (k 2) and the heat-flow output powers of the second highest peak (P 2), together with the derived parameter inhibitory ratio (I, %), it could be quickly concluded that the tested S. miltiorrhizae samples from different sources in China exhibited strong antibacterial effects on E. coli and the samples from Beijing city exhibited the strongest anti-E. coli effects, which might be used as novel and underlying antibacterial candidates for the resistance of E. coli to the existing drugs in practice. This study provides a useful tool and helpful idea to accurately and rapidly evaluate the antibacterial effects of some complex matrices, offering some references for exploring new antibacterial agents.

show abstract

Impact of beta-cypermethrin on soil microbial community associated with its bioavailability: A combined study by isothermal microcalorimetry and enzyme assay techniques

Cited by 42 publications

References 36 publications

Monooxygenase, a Novel Beta-Cypermethrin Degrading Enzyme from Streptomyces sp

Monooxygenase, a Novel Beta-Cypermethrin Degrading Enzyme from Streptomyces sp

Bioremediation of β-cypermethrin and 3-phenoxybenzaldehyde contaminated soils using Streptomyces aureus HP-S-01

Antibacterial evaluation of Salvia miltiorrhizae on Escherichia coli by microcalorimetry coupled with chemometrics

Contact Info

Product

Resources

About